EGCG Attenuates Uric Acid-Induced Inflammatory and Oxidative Stress Responses by Medicating the NOTCH Pathway

Xie, Hua; Sun, Jianqin; Chen, Yanqiu; Zong, Min‐Hua; Li, Shijie; Wang, Yan

doi:10.1155/2015/214836

Cited by 52 publications

(39 citation statements)

References 32 publications

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“…As described in previous studies [31, 32], the statistical analysis was performed with the Statistical Package for Social Sciences (SPSS version 13.0; IBM Analytics, Chicago, IL, USA). All the data were expressed as mean ± SD (standard deviation, SD) and the difference was analyzed by a one-way ANOVA test.…”

Section: Methodsmentioning

confidence: 99%

Renalase as a Novel Biomarker for Evaluating the Severity of Hepatic Ischemia‐Reperfusion Injury

Guo

Liu

et al. 2016

Oxidative Medicine and Cellular Longevity

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Hepatic ischemia-reperfusion (I/R) injury is a serious complication in clinical practice. However, no efficient biomarkers are available for the evaluation of the severity of I/R injury. Recently, renalase has been reported to be implicated in the I/R injury of various organs. This protein is secreted into the blood in response to increased oxidative stress. To investigate the responsiveness of renalase to oxidative stress, we examined the changes of renalase in cell and mouse models. We observed a significant increase of renalase expression in HepG2 cells in a time- and dose-dependent manner when treated with H2O2. Renalase expression also increased significantly in liver tissues that underwent the hepatic I/R process. The increased renalase levels could be efficiently suppressed by antioxidants in vitro and in vivo. Furthermore, serum renalase levels were significantly increased in the mouse models and also efficiently suppressed by antioxidants treatment. The variation trends are consistent between renalase and liver enzymes in the mouse models. In conclusion, renalase is highly sensitive and responsive to oxidative stress in vitro and in vivo. Moreover, renalase can be detected in the blood. These properties make renalase a highly promising biomarker for the evaluation of the severity of hepatic I/R injury.

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Section: Methodsmentioning

confidence: 99%

Renalase as a Novel Biomarker for Evaluating the Severity of Hepatic Ischemia‐Reperfusion Injury

Guo

Liu

et al. 2016

Oxidative Medicine and Cellular Longevity

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“…Notch signaling pathway is an evolutionarily conserved signaling pathway that controls the fate of cells and maintenance of stemness [37]. EGCG could inhibit Notch signaling pathway [22][23][24]38], Especially, it was found that Notch signaling pathway inactivation can effectively reduce cisplatin induced osteosarcoma stem cells production and reduce the resistance of osteosarcoma cells [39], which suggested that Notch signaling pathway play a role in OS drug resistance and stemness. In our research, by detecting downstream target genes of Notch signaling (Hes1 and Hey1), it was proved that V7 overexpression could further activated Notch signaling and EGCG treatment could inhibit the Notch signaling pathway in OSCs.…”

Section: Disscussionmentioning

confidence: 99%

SOX2OT variant 7 contributes to the synergistic interaction between EGCG and Doxorubicin to kill osteosarcoma via autophagy and stemness inhibition

Wang

Chen

Zhu

2018

J Exp Clin Cancer Res

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Background: Doxorubicin is the preferred chemotherapeuticdrug for osteosarcoma treatment of which clinical efficacy is limited because of its chemo-resistance and cardiac toxicity. It is necessary to develop the combination regimen with complementary molecular mechanisms to reduce the side effects and enhance sensitivity of Doxorubicin. EGCG is a polyphenol in green tea with antitumor bioactivity,which has been found that its combination with certain chemotherapeutic drugs could improve the antitumor efficiency. Methods: In this study, MTT assay was used to detect the cell growth inhibition The CD133+/CD44+ cells were isolated from U2OS and SaoS2 cell lines using magnetic-activated cell sorting and identified by flow cytometry analysis. qRT-PCR was used for determining the relative mRNA levels of key genes. Immunofluorescence was performed to evaluate the autophagy flux alterations. Self-renewal ability was accessed by sphere-forming assay. Tumorigenicity in nude mice was preformed to evaluate tumorigenicity in vivo. Results: We found that EGCG targeting LncRNA SOX2OT variant 7 produced synergistic effects with Doxorubicin on osteosarcoma cell growth inhibition. On the one hand, EGCG could reduce the Doxorubicin-induced pro-survival autophagy through decreasing SOX2OT variant 7 to improve the growth inhibition of Doxorubicin. On the other hand, EGCG could partially inactivate Notch3/DLL3 signaling cascade targeting SOX2OT variant 7 to reduce the stemness then abated drug-resistance of osteosarcoma cells.

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“…The possible mechanisms by which SUA accelerated arterial stiffness have been proposed as follows. First, uric acid stimulates inflammatory pathways by nuclear factor-κB, Notch-1, and chemoattractant protein 1 signals [26,27]. Second, uric acid increases production of reactive oxygen species and decreases the bioavailability of nitric oxide in vascular endothelial cells and induces endothelial dysfunction [27,28].…”

Section: Discussionmentioning

confidence: 99%

Association of Serum Uric Acid with Arterial Stiffness in Peritoneal Dialysis Patients

Liu

et al. 2018

Kidney Blood Press Res

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Background/Aims: Serum uric acid (SUA) has been proposed as a mediator associated with increased cardiovascular risk and arterial stiffness. However, evidence on the association between SUA and arterial stiffness in peritoneal dialysis (PD) patients is lacking. The aim of this study was to examine the relationship between SUA and arterial stiffness in PD patients. Methods: The patients who performed vascular profiler test from January 1, 2014 to October 31, 2016, and with SUA values were enrolled. Arterial stiffness was evaluated by brachial-ankle pulse wave velocity (baPWV). The relationship between SUA and baPWV was tested by multiple linear regression models. Results: Of 645 PD patients, mean SUA was 6.80 (±1.29) mg/dL, mean baPWV was 1713 (±505) cm/s. In fully adjusted linear regression models, higher SUA was significantly associated with higher baPWV in young [standardized coefficients (β), 0.085; 95% confidence interval (95% CI), 0.013 to 0.130; P=0.02] but not in elderly (β, -0.194; 95% CI, -0.774 to 0.093; P=0.1) PD patients. In gender-stratified models of young patients, there was a significant association between SUA and baPWV in male (β, 0.115; 95% CI, 0.015 to 0.182; P=0.02) but not in female. Male in the highest gender-specific SUA quartile had a higher baPWV than those in the lowest quartile (β, 0.132; 95% CI, 0.011 to 0.209; P=0.03). This gender difference was reversed when selecting male patients with lower SUA levels (quartile 1 and 2) and female patients with higher SUA levels (quartile 3 and 4). Conclusion: SUA was positively associated with baPWV in young PD patients, and this association was significant in males but not in females, which is possibly explained by the higher SUA level in males than in females.

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EGCG Attenuates Uric Acid-Induced Inflammatory and Oxidative Stress Responses by Medicating the NOTCH Pathway

Cited by 52 publications

References 32 publications

Renalase as a Novel Biomarker for Evaluating the Severity of Hepatic Ischemia‐Reperfusion Injury

Renalase as a Novel Biomarker for Evaluating the Severity of Hepatic Ischemia‐Reperfusion Injury

SOX2OT variant 7 contributes to the synergistic interaction between EGCG and Doxorubicin to kill osteosarcoma via autophagy and stemness inhibition

Association of Serum Uric Acid with Arterial Stiffness in Peritoneal Dialysis Patients

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