Eficácia do eugenol extraído da planta Eugenia aromática como anestésico para realização de biometrias em adultos de tilápia do Nilo (Oreochromis niloticus)

Abstract

RESUMO.Os anestésicos figuram como poderosa ferramenta na aquicultura, atuando na redução do estresse e mortalidade usualmente causados pelo manejo. O objetivo deste trabalho foi avaliar o efeito de diferentes concentrações de eugenol em adultos de tilápia e determinar a concentração ideal para realização de uma biometria. Foram avaliadas seis concentrações (30, 60, 120, 180, 240 e 300 mg L -1 ). Para cada tratamento, 12 peixes (comprimento médio: 32,7 ± 3,0; peso médio: 557,0 ± 160 g), foram imersos individualmente na solução anestésica durante 10 min. Durante a indução anestésica, foram monitorados todos os estágios de anestesia e ao atingir o estágio de anestesia profunda, foi monitorado o número de batimentos operculares durante o minuto seguinte. Após a indução anestésica, os peixes foram transferidos para aquário contendo água sem anestésico e aferido o tempo de recuperação. A concentração de 60 mg L ABSTRACT. Efficacy of eugenol extracted from the plant Eugenia aromatica as an anesthetic for the biometry procedures in Nile tilapia (Oreochromis niloticus) adults.Anesthetics represent a powerful tool in aquaculture, working to reduce stress and mortality commonly caused by handling. The objective this work was evaluate the effect of various concentrations of eugenol in adults of Nile tilapia and determine its optimal concentration for biometry procedures. Six concentrations (30, 60, 120, 180, 240 e 300 mg L -1 ) were tested. For each treatment, 12 fishes (average length: 32.7 ± 3.0; average weight: 557.0 ± 160 g) were individually immersed in anesthetic solution for 10 min. All stages of anesthesia were monitored during anesthetic induction, and upon profound anesthesia the opercular beat rate was monitored for the next minute. After anesthetic induction, the fishes were transferred to an aquarium containing water without anesthetic solution, and recovery time was measured. The 60 mg L concentration reached this stage more rapidly (77.8 seconds). Results suggest that the optimal concentration of eugenol for biometry procedures is 120 mg L -1 , and that increase anesthetic dosage results in reduced opercular beat rate.

Use of eugenol as an anaesthetic for Geophagus brasiliensis juveniles

“…In this study, the concentration of 80 mg L -1 is the most appropriate, though having had no significant difference between doses of 50 and 100 mg L -1 , because the times for achieve the different stages were more homogeneous. Beyond the verification of the times of anesthesia and recovery of different drugs and their possible concentrations, studies have been conducted to minimize the stress caused by practices that require the handling of fish in farms (MOREIRA et al, 2010).…”

“…In Brazil there are recent reports of using eugenol (2-methoxy-4-(2-propenyl)phenol), the main component of clove oil (70-90% by weight) or just clove oil as an anesthetic for several species like Centropomus undecimalis (BERNARDES JÚNIOR, et al, 2013), Pimelodus britskii (BERTOZI JÚNIOR et al, 2014), Rhamdia quelen (CUNHA et al, 2010;GOMES et al, 2011;BECKER et al, 2012;SUTILI et al, 2014), Oreochromis niloticus (MOREIRA et al, 2010;, Cyprinus carpio (BITTENCOURT et al, 2013), Colossoma macropomum (INOUE et al, 2011), Pseudoplatystoma reticulatum (SANTOS SANCHEZ et al, 2014b), Gymnotus aff. inaequilabiatus (PÁDUA et al, 2012), Brycon hilarri (FABIANI et al, 2013) among others.…”

<b>Digestible protein requirement for Nile tilapia fed with rations based on soybean meal and corn

“…The average dissolved oxygen in experimental tanks was equal to 6.9 ± 1.5 mg L At the end of the experiment, the fish were anesthetized (eugenol 75 mg l -¹) (Moreira, Teixeira, Carreiro & Moreira, 2010), weighed and measured (height, width, length and head) individually for the assessment of body parameters, chemical composition of the carcass and liver histology. In each experimental unit three animals were selected and randomly slaughtered to assessment of body yield parameters and histological evaluation (Vicentini et al, 2005) For the study of the morphology and integrity of the hepatic tissue, the procedures described by Graciano, Natali, Vidal, Michelato, Righetti and Furuya (2010) The final results of the evaluated parameters were submitted to variance analysis with significance level of 5% and in the case of statistical difference were applied regression analysis, using the SAEG program.…”

Eugenol como anestésico para oscar, Astronotus ocellatus

“…Variáveis N SL AL AP AC mal, fato não observado na utilização do eugenol na sedação de algumas outras espécies de peixe (Cunha et al, 2010;Moreira et al, 2010).…”