2022
DOI: 10.3389/fbioe.2022.805996
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Abstract: Nanomaterials have been widely studied for their potential to become the new generation of nanocarriers in gene transfection, yet it remains still difficult to apply them efficiently and succinctly to plant cells. Poly (2-(N,N-dimethylamino) ethyl methacrylate) (PDMAEMA), which possesses temperature and pH dual-sensitivity, has largely been applied in animal cells, but it is rarely involved in plant cells. As a proof of concept, PDMAEMA as a gene carrier is incubated with plasmid GFP (pGFP) to explore its tran… Show more

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Cited by 2 publications
(1 citation statement)
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“…Clarity Western Substrate (BIO-RAD) was used for chemiluminescent detection of the GFP protein. The detection was performed using a LI-COR Odyssey Infrared Imaging System (Tanon, 5,200, China) ( An et al., 2022 ).…”
Section: Methodsmentioning
confidence: 99%
“…Clarity Western Substrate (BIO-RAD) was used for chemiluminescent detection of the GFP protein. The detection was performed using a LI-COR Odyssey Infrared Imaging System (Tanon, 5,200, China) ( An et al., 2022 ).…”
Section: Methodsmentioning
confidence: 99%