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Cited by 26 publications
(24 citation statements)
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References 29 publications
(58 reference statements)
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“…To test this assumption, we investigated the ability of CVB3-HL1 to infect and replicate in mouse myoblast C2C12 cells and CHO-K1 cells. CHO-K1 cells do not express CAR (57), whereas a low level of CAR expression in C2C12 cells was reported previously (32,44). Real-time RT-PCR analysis, however, demonstrated an absence of CAR expression in the C2C12 cells used in this study (results not shown).…”
Section: Resultscontrasting
confidence: 49%
“…To test this assumption, we investigated the ability of CVB3-HL1 to infect and replicate in mouse myoblast C2C12 cells and CHO-K1 cells. CHO-K1 cells do not express CAR (57), whereas a low level of CAR expression in C2C12 cells was reported previously (32,44). Real-time RT-PCR analysis, however, demonstrated an absence of CAR expression in the C2C12 cells used in this study (results not shown).…”
Section: Resultscontrasting
confidence: 49%
“…The dystrophin expression cassette was subsequently harvested from this plasmid. The LacZ expression cassette consisted of the Escherichia coli LacZ gene controlled by the Rous sarcoma virus long terminal repeat (RSV) promoter [20], and the CAR expression cassette consisted of the human CAR cDNA controlled by the RSV promoter [11]. The whole multiple cloning site of pBluescript was replaced by the sequence ApaI-PacI-NotI-PacI-BamHI by ligation of the synthetic oligonucleotide, restriction enzyme digestion and self-ligation; the generated plasmid was named pPN.…”
Section: Construction Of Hdadsmentioning
confidence: 99%
“…The secondary antibody was biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA, 1 : 200), and the stained sections were visualized by horseradish peroxidase. Serial frozen sections from muscle injected with HDAdLacZ-dys or HDAdCAR-dys were stained for detection of β-gal or CAR as described previously, respectively [11]. For detection of β-gal, frozen sections were fixed with 2% paraformaldehyde and 0.2% glutaraldehyde in phosphate-buffered saline (PBS), and stained with 0.1% X-gal in 5 mM potassium ferricyanide, 5 mM potassium ferrocyanide and 2 mM MgCl 2 .…”
Section: Histological Examination and Immunostainingmentioning
confidence: 99%
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