Efficient protein digestion using immobilized trypsin onto tannin modified Fe 3 O 4 magnetic nanoparticles

Atacan, Keziban; Çakıroğlu, Bekir; Özacar, Mahmut

doi:10.1016/j.colsurfb.2017.04.055

Cited by 51 publications

(30 citation statements)

References 55 publications

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“…Accordinlgy, the CNPs have a graphite-like structure, with a broad diraction peak around 23.54° and interlayer spacing of 0.36 nm [36]. Six characteristic peaks of Fe 3 O 4 NPs (2θ = 30.2, 35.6, 43.4, 53.7, 57.3 and 62.8) were also observed in both XRD spectra of Fe 3 O 4 NPs and CNPs@Fe 3 O 4 NCs, confirming the formation of Fe 3 O 4 NPs on the surface of CNPs [37, 38]. The UV−Vis absorption spectra (ESM Fig.…”

Section: Resultsmentioning

confidence: 87%

Magnetic carbon nanocomposites as a MALDI co-matrix enhancing MS-based glycomics

et al. 2018

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More than 50% of all known proteins are glycosylated, which is critical for many biological processes such as protein folding and signal transduction. Glycosylation has proven to be associated with different mammalian diseases such as breast and liver cancers. Therefore, characterization of glycans is highly important to facilitate a better understanding of the development and progression of many human diseases. Although matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) offers several advantages such as ease of operation and short analysis times, however, due to the complexity of glycan structures and their low ionization efficiency, there are still challenges that need to be addressed to achieve sensitive glycan analysis. Here, magnetic carbon nanocomposites (CNPs@FeO NCs) were used as a new MALDI matrix or co-matrix for the analysis of glycans derived from different model glycoproteins and human blood serum samples. The addition of CNPs@FeO NCs to the matrix significantly enhanced glycan signal intensity by several orders of magnitude, and effectively controlled/reduced/eliminated in-source decay (ISD) fragmentation. The latter was attained by modulating CNPs@FeO NCs concentrations and allowed the simultaneous study of intact and fragmented glycans, and pseudo-MS analysis. Moreover, CNPs@FeO NCs was also effectively employed to desalt samples directly on MALDI plate, thus enabling direct MALDI-MS analysis of unpurified permethylated glycans derived from both model glycoproteins and biological samples. On-plate desalting enhanced sensitivity by reducing sample loss. Graphical abstract ᅟ.

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Section: Resultsmentioning

confidence: 87%

Magnetic carbon nanocomposites as a MALDI co-matrix enhancing MS-based glycomics

et al. 2018

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“…The surface modification of SPIONs with different biological agents can NPs improve their properties, including stability, water solubility, biocompatibility, and cellular uptake, which are highly needed for biomedical applications, such as drug delivery, imaging, and biosensors. Different kinds of amino acids, sugars, proteins, polymers, and nucleic acids are used for modification of NPs.…”

Section: Introductionmentioning

confidence: 99%

“…In some cases, incorporation of magnetic NPs could improve bioactivity of the scaffolds, these magnetite NPs enhance the biological properties of cells that are needed for bone regeneration including cell mineralization. 30 The surface modification of SPIONs with different biological agents can NPs improve their properties, including stability, water solubility, biocompatibility, and cellular uptake, [31][32][33] which are highly needed for biomedical applications, such as drug delivery, imaging, and biosensors. Different kinds of amino acids, 34 sugars, 35,36 proteins, 37 polymers, 38,39 and nucleic acids 40 are used for modification of NPs.…”

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confidence: 99%

Incorporation of SPION‐casein core‐shells into silk‐fibroin nanofibers for cardiac tissue engineering

Nazari

Heirani‐Tabasi

Hajiabbas

et al. 2019

J of Cellular Biochemistry

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Mimicking the structure of extracellular matrix (ECM) of myocardium is necessary for fabrication of functional cardiac tissue. The superparamagnetic iron oxide nanoparticles (SPIONs, Fe3O4), as new generation of magnetic nanoparticles (NPs), are highly intended in biomedical studies. Here, SPION NPs (1 wt%) were synthesized and incorporated into silk‐fibroin (SF) electrospun nanofibers to enhance mechanical properties and topography of the scaffolds. Then, the mouse embryonic cardiac cells (ECCs) were seeded on the scaffolds for in vitro studies. The SPION NPs were studied by scanning electron microscope (SEM), X‐ray diffraction (XRD), and transmission electron microscope (TEM). SF nanofibers were characterized after incorporation of SPIONs by SEM, TEM, water contact angle measurement, and tensile test. Furthermore, cytocompatibility of scaffolds was confirmed by 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT) assay. SEM images showed that ECCs attached to the scaffolds with elongated morphologies. Also, the real‐time PCR and immunostaining studies approved upregulation of cardiac functional genes in ECCs seeded on the SF/SPION‐casein scaffolds including GATA‐4, cardiac troponin T, Nkx 2.5, and alpha‐myosin heavy chain, compared with the ones in SF. In conclusion, incorporation of core‐shells in SF supports cardiac differentiation, while has no negative impact on ECCs' proliferation and self‐renewal capacity.

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“…The modification of Fe 3 O 4 NPs with surfactants, such as sodium dodecyl sulfate (SDS), has been reported as an appropriate and facile way to improve Fe 3 O 4 performance. [28][29][30] The Fe 3 O 4 NPs modified with SDS have been reported in 2011 for removal of safranin O dye from aqueous solutions. 30 Faraji et al reported the SDS-coated Fe 3 O 4 NPs as a solid-phase extraction adsorbent for the extraction of Hg(II) from water samples.…”

Section: Introductionmentioning

confidence: 99%

“…29 Furthermore, high-efficiency extraction of fluoxetine using the Fe 3 O 4 NPs modified with SDS from aquatic and urine samples have been reported in 2011. 28 In this study, we aimed to modify the Fe 3 O 4 NPs surface with sodium dodecyl sulfate as a facile modification way to improve the adsorption efficiency of the magnetic nanoparticles, which exhibit a high efficiency for tolonium chloride removal from aqueous solutions. The effect of different parameters, such as pH, dye concentration, temperature, and ions intervention on process performance was investigated.…”

Section: Introductionmentioning

confidence: 99%

Removal of Cationic Tolonium Chloride Dye Using Fe3 O4 Nanoparticles Modified with Sodium Dodecyl Sulfate

Abedi

Ahmadmoazzam

Jaafarzadeh

2018

Chem.Biochem.Eng.Q.

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In this study, Fe 3 O 4 nanoparticles modified with sodium dodecyl sulfate (Fe 3 O 4-SDS) were used for the adsorption of cationic tolonium chloride (TC) dye from aqueous solution. The effects of temperature, pH, amount of nanoparticles and SDS concentration, stirring time, and interfering ions on dye adsorption were investigated. For TC, the maximum removal was 98 % in the best conditions (pH = 6; 0.6 g Fe 3 O 4-SDS; 30 °C temperature, and 3 min stirring time). Data from the sorption kinetic studies showed the best fitting of the pseudo-second-order model for adsorption of the dye. The Langmuir isotherm showed better fitting of the data for TC adsorption by Fe 3 O 4-SDS compared with the other models. The Fe 3 O 4-SDS nanoparticles are easily synthesizable, magnetically recoverable, and regenerable, which increases their practical applications. Additionally, NaCl showed no significant impact on the dye removal up to 0.8 molar concentration, which increases the industrial application of the process.

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Efficient protein digestion using immobilized trypsin onto tannin modified Fe 3 O 4 magnetic nanoparticles

Cited by 51 publications

References 55 publications

Magnetic carbon nanocomposites as a MALDI co-matrix enhancing MS-based glycomics

Magnetic carbon nanocomposites as a MALDI co-matrix enhancing MS-based glycomics

Incorporation of SPION‐casein core‐shells into silk‐fibroin nanofibers for cardiac tissue engineering

Removal of Cationic Tolonium Chloride Dye Using Fe3 O4 Nanoparticles Modified with Sodium Dodecyl Sulfate

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