Efficient production of ethanol from crude glycerol by a Klebsiella pneumoniae mutant strain

Oh, Baek-Rock; Seo, Jeong-Woo; Heo, Sun-Yeon; Hong, Won-Kyung; Luo, Lian Hua; Joe, Min-Ho; Park, Don-Hee; Kim, Chul Ho

doi:10.1016/j.biortech.2010.12.007

Cited by 107 publications

(50 citation statements)

References 20 publications

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“…The λ Red recombinase and the FLP recombinase were expressed by the helper plasmids pKD46 [12] and pCP20 [13], respectively; replication of these plasmids is temperature sensitive, and the plasmids can thus be easily eliminated. Microbial cells were grown in LB [yeast extract (Difco), 0.5% (w/v); Bacto-tryptone (Difco), 1.0% (w/v); and NaCl, 1.0% (w/v)] or germ medium [14] supplemented with appropriate antibiotics (ampicillin [50 μg ml −1 ] and/or tetracycline [10 μg ml −1 for E. coli and 50 μg ml −1 for K. pneumoniae]). Germ medium contained 20 gl −1 glycerol, 2 gl −1 (NH 4 To make an ldhA deletion mutant, 900-bp DNA sequences located upstream and downstream of ldhA were amplified by PCR using oligonucleotides L1 (5′-CAGCCAGACGGGAA TAGCTT-3′) and L2 (5′-CGCCAATTTTCTGGTGCTTCAGATATCGCCTCAAGGTC GACGTTGTTAA-3′; the bases in italics indicate an EcoRV site) for the upstream region a n d L 3 ( 5 ′ -T TA A C A A C G T C G A C C T T G A G G C G ATAT C T G A A G C A C C A GAAAATTGGCG-3′; the bases in italics indicate an EcoRV site) and L4 (5′-AGCTC GATGGTTCGGCGATT-3′) for the downstream region.…”

Section: Methodsmentioning

confidence: 99%

Optimization of Culture Conditions for 1,3-Propanediol Production from Glycerol Using a Mutant Strain of Klebsiella pneumoniae

Seo

Heo

et al. 2011

Appl Biochem Biotechnol

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In the present work, mutant strains of Klebsiella pneumoniae with deletions of the als gene encoding acetolactate synthase involved in synthesis of 2,3-butanediol, the ldhA gene encoding lactate dehydrogenase required for lactate synthesis, or both genes, were prepared. Production of 1,3-propanediol (1,3-PD) from glycerol was enhanced in the ldhA mutant strain (ΔldhA), but lower in Δals or Δals ΔldhA mutant strains compared to the parent strain, concomitant with a reduction in the glycerol consumption rate, indicating that deletion of ldhA alone was useful to improve 1,3-PD production. Fed-batch fermentation analysis revealed that, in the ΔldhA mutant strain, 1,3-PD production was higher at low pH than at neutral pH; the reverse was true for the parent strain. Further optimization of culture conditions, by variation of aeration and glycerol feed rates, dramatically improved the production of 1,3-PD by the mutant strain. The maximum level attained was 102.7 g l(-1) of 1,3-PD from glycerol.

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Section: Methodsmentioning

confidence: 99%

Optimization of Culture Conditions for 1,3-Propanediol Production from Glycerol Using a Mutant Strain of Klebsiella pneumoniae

Seo

Heo

et al. 2011

Appl Biochem Biotechnol

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“…Fermentation of glycerol by Klebsiella planticola, isolated from the rumen of red deer (Cervus elaphus) resulted in ethanol and formate as major products at concentrations in the range of 30-32 mmol/L [42]. K. pneumoniae and Kluyvera cryocrescens S26 have a high capacity to produce ethanol from CG [43,44]. The genetic modifications in K. pneumoniae were achieved by inactivating lactate dehydrogenase and introducing pyruvate decarboxylase and aldehyde dehydrogenase from Zymomonas mobilis.…”

Section: Ethanolmentioning

confidence: 99%

Biorefinery for Glycerol Rich Biodiesel Industry Waste

2016

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The biodiesel industry has the potential to meet the fuel requirements in the future. A few inherent lacunae of this bioprocess are the effluent, which is 10 % of the actual product, and the fact that it is 85 % glycerol along with a few impurities. Biological treatments of wastes have been known as a dependable and economical direction of overseeing them and bring some value added products as well. A novel eco-biotechnological strategy employs metabolically diverse bacteria, which ensures higher reproducibility and economics. In this article, we have opined, which organisms and what bioproducts should be the focus, while exploiting glycerol as feed.

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“…Glycerol로 부터 ethanol 생산은 세균 [5,6,14]과 효모 [12,13]를 이용하는 방법이 보고되고 있는데, 세균을 이용하는 방 법은 공기가 없는 조건에서 anaerobic fermentation으로 이루 어지며, 대사경로와 redox balance 등이 어느 정도 잘 이해되 어지고 있다 [18]. 반면에, 효모의 경우에는 일반적으로 glyceol 이 fermentable 탄소원으로 사용되어지지 않는 것으로 알려져 왔음에도 불구하고, 효모 Pachysolen tannophilus (P. tannophilus)가 glycerol을 이용하여 ethanol을 생성한다는 보고가 발표 되었다 [12,13].…”

Section: 서 론unclassified

Ethanol Production from Glycerol using Pachysolen tannophilus in a Surface-aerated Fermentor

Kim¹,

Choi²,

Kang³

et al. 2013

Journal of Life Science

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We investigated ethanol production from glycerol after screening of the yeast Pachysolen tannophilus ATCC 32691. For yeast to produce ethanol form glycerol, it is important that aeration is finely controlled. Therefore, we attempted to produce ethanol using a surface-aerated fermentor. When 880 ml of YPG medium (1% yeast extract, 2% peptone, 2% glycerol) was used to produce ethanol, the optimal aeration conditions for ethanol production were a surface aeration rate and agitation speed of 500 ml/min and 300 rpm, respectively. In a fed-batch culture, the maximum ethanol production and the maximum ethanol yield from glycerol (Ye/g) was 5.74 g/l and 0.166, respectively, after 90 hr using the surface-aerated fermentor.

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Efficient production of ethanol from crude glycerol by a Klebsiella pneumoniae mutant strain

Cited by 107 publications

References 20 publications

Optimization of Culture Conditions for 1,3-Propanediol Production from Glycerol Using a Mutant Strain of Klebsiella pneumoniae

Optimization of Culture Conditions for 1,3-Propanediol Production from Glycerol Using a Mutant Strain of Klebsiella pneumoniae

Biorefinery for Glycerol Rich Biodiesel Industry Waste

Ethanol Production from Glycerol using Pachysolen tannophilus in a Surface-aerated Fermentor

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