Efficient production of 1,3-propanediol from crude glycerol by repeated fed-batch fermentation strategy of a lactate and 2,3-butanediol deficient mutant of Klebsiella pneumoniae

Oh, Baek-Rock; Lee, Sung-Mok; Heo, Sun-Yeon; Seo, Jeong-Woo; Kim, Chul Ho

doi:10.1186/s12934-018-0921-z

Cited by 41 publications

(35 citation statements)

References 28 publications

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“…During the optimization of the initial glucose concentration, values of 140, 160, 180, 200, and 220 g/L were evaluated, setting the pH and temperature to the optimal values. During the optimization of the inoculation volume, fermenters containing 2.7, 2.4, and 2.1 L of fresh improved fermentation medium with the optimal initial glucose concentration were seeded with 300, 600, and 900 mL of fresh seed, respectively [19]. Similarly, the pH and temperature were both adjusted to the optimal values.…”

Section: Methodsmentioning

confidence: 99%

Kinetic characteristics of long‐term repeated fed‐batch (LtRFb) l‐lactic acid fermentation by a Bacillus coagulans strain

Zhang

Liu

Xiao

et al. 2020

Engineering in Life Sciences

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Application of degradable plastics is the most critical solution to plastic pollution. As the precursor of biodegradable plastic PLA (polylactic acid), efficient production of l-lactic acid is vital for the commercial replacement of traditional plastics. Bacillus coagulans H-2, a robust strain, was investigated for effective production of l-lactic acid using long-term repeated fed-batch (LtRFb) fermentation. Kinetic characteristics of l-lactic acid fermentation were analyzed by two models, showing that cell-growth coupled production gradually replaces cellmaintenance coupled production during fermentation. With the LtRFb strategy, l-lactic acid was produced at a high final concentration of 192.7 g/L, on average, and a yield of up to 93.0% during 20 batches of repeated fermentation within 487.5 h. Thus, strain H-2 can be used in the industrial production of l-lactic acid with optimization based on kinetic modeling.

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Section: Methodsmentioning

confidence: 99%

Kinetic characteristics of long‐term repeated fed‐batch (LtRFb) l‐lactic acid fermentation by a Bacillus coagulans strain

Zhang

Liu

Xiao

et al. 2020

Engineering in Life Sciences

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“…The mobile phase was 2.5 mM H 2 SO 4 , and the flow rate was 0.6 mL/min. The column and cell temperatures were 65 °C and 45 °C, respectively [32]. Total nitrogen concentration was measured using a HS-TN (CA)-L kit (concentration range: 1 to 50 mg/L; Humas, Korea) with a HS-2300 plus water analyzer.…”

Section: Metabolites and Total Nitrogen Analysismentioning

confidence: 99%

Enhancement of 1,3-propanediol production from industrial by-product by Lactobacillus reuteri CH53

Wang

Heo

et al. 2020

Microb Cell Fact

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Background: 1,3-propanediol (1,3-PDO) is the most widely studied value-added product that can be produced by feeding glycerol to bacteria, including Lactobacillus sp. However, previous research reported that L. reuteri only produced small amounts and had low productivity of 1,3-PDO. It is urgent to develop procedures that improve the production and productivity of 1,3-PDO. Results:We identified a novel L. reuteri CH53 isolate that efficiently converted glycerol into 1,3-PDO, and performed batch co-fermentation with glycerol and glucose to evaluate its production of 1,3-PDO and other products. We optimized the fermentation conditions and nitrogen sources to increase the productivity. Fed-batch fermentation using corn steep liquor (CSL) as a replacement for beef extract led to 1,3-PDO production (68.32 ± 0.84 g/L) and productivity (1.27 ± 0.02 g/L/h) at optimized conditions (unaerated and 100 rpm). When CSL was used as an alternative nitrogen source, the activity of the vitamin B12-dependent glycerol dehydratase (dhaB) and 1,3-propanediol oxidoreductase (dhaT) increased. Also, the productivity and yield of 1,3-PDO increased as well. These results showed the highest productivity in Lactobacillus species. In addition, hurdle to 1,3-PDO production in this strain were identified via analysis of the half-maximal inhibitory concentration for growth (IC50) of numerous substrates and metabolites. Conclusions:We used CSL as a low-cost nitrogen source to replace beef extract for 1,3-PDO production in L. reuteri CH53. These cells efficiently utilized crude glycerol and CSL to produce 1,3-PDO. This strain has great promise for the production of 1,3-PDO because it is generally recognized as safe (GRAS) and non-pathogenic. Also, this strain has high productivity and high conversion yield.

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“…Repeated fed-batch fermentation is to pump out most of fermentation broths and remain an amount at the end of fed-batch fermentation, followed by fresh medium. To some extents, repeated fedbatch fermentation avoids the seed culture time between two fed-batches and enhances the overall products productivity, which is great promising for industrial 1,3-PDO production (Oh et al,2018a). Considering the sustainable operations and overall productivity in industry, however, there are also some specific barriers and potential problems to repeated fed-batch fermentation.…”

Section: Introductionmentioning

confidence: 99%

“…pneumoniae only, which is pathogenic and vitamin B 12 -dependent. Additionally, low 1,3-PDO concentration (66 g/L) (Xue et al,2010) and more by-products were obtained (37.5 g/L 2.3propanediol) (Oh et al,2018a). More importantly, the number of cycles is worrying, as the seed broths of each cycle come from the last cycle of fermentation, in which the microbial cells are at the decline phase.…”

Section: Introductionmentioning

confidence: 99%

Sequential fed-batch fermentation of 1,3-propanediol from glycerol by Clostridium butyricum DL07

Wang

Zhou

Shen

et al. 2020

Preprint

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The demand for 1,3-propanediol (1,3-PDO) has increased sharply due to its role as a monomer for the synthesis of polytrimethylene terephthalate (PTT). Although Clostridium butyricum is considered to be one of the most promising bio-producers for 1,3-PDO, its low productivity hinders its application on industrial scale because of the longer time needed for anaerobic cultivation. In this study, an excellent mutant of C. butyricum (DL07) was obtained with high-level titer and productivity of 1,3-PDO, i.e. 104.78 g/L and 3.38 g/(L*h) vs. 94.23 g/L and 3.04 g/(L*h) using pure or crude glycerol as substrate in fed-batch fermentation, respectively. Furthermore, a novel sequential fed-batch fermentation was investigated, in which the next bioreactor was inoculated by C. butyricum DL07 cells growing at exponential phase in the prior bioreactor. It could run steadily for at least eight cycles. The average concentration of 1,3-PDO in eight cycles was 84.62 g/L with the average productivity of 3.05 g/(L*h).The sequential fed-batch fermentation could achieve semi-continuous production of 1,3-PDO with higher productivity than repeated fed-batch fermentation, and would greatly contribute to the industrial production of 1,3-propanediol by C. butyricum.

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Efficient production of 1,3-propanediol from crude glycerol by repeated fed-batch fermentation strategy of a lactate and 2,3-butanediol deficient mutant of Klebsiella pneumoniae

Cited by 41 publications

References 28 publications

Kinetic characteristics of long‐term repeated fed‐batch (LtRFb) l‐lactic acid fermentation by a Bacillus coagulans strain

Kinetic characteristics of long‐term repeated fed‐batch (LtRFb) l‐lactic acid fermentation by a Bacillus coagulans strain

Enhancement of 1,3-propanediol production from industrial by-product by Lactobacillus reuteri CH53

Sequential fed-batch fermentation of 1,3-propanediol from glycerol by Clostridium butyricum DL07

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