Efficient plant regeneration and histological evaluations of regenerants through organogenesis and somatic embryogenesis in Spermacoce hispida L.—An underutilized medicinally important plant

Deepak, K.V.; Ivin, J. Johnny Subakar; Narayanan, G. Sathiya; Prakash, M.; Murugan, Shanmugaraj Bala; Anandan, R.

doi:10.1016/j.indcrop.2019.03.067

Cited by 8 publications

(8 citation statements)

References 42 publications

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“…6). Regardless of the acclimatizing environment, similar observations on survival rate and adaptability were recorded from organogenesis and somatic embryogenesis derived plants (Bhagya et al 2013;Deepak et al 2019).…”

Section: Hardening Of Somatic Plantssupporting

confidence: 59%

“…In this direct shoot organogenesis involving nodal segments has been successfully established from in vivo grown seedlings of C. zizanioides. Several reports have authenticated the superior role of nodal explant over the other types to accomplish micropropagation in medicinal plants such as Mentha piperita (Sujana 2011), Withania somnifera (Fatima 2012), Agastache foeniculum (Moharami 2014), Teucrium scorodonia (Makowczyńska 2016), and Spermacoce hispida(Deepak et al 2019). Earlier literature has proved the existence of positive correlations among explant types, the shoot proliferation rate, and in uences of cytokinin(Sajid et al 2011;Dantu and Bhojwani 2013;Lebedev et al 2018).…”

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confidence: 99%

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High frequency plant regeneration of Chrysopogon zizanioides via organogenesis and somatic embryogenesis-an underutilized pharmaceutically valuable biofuel plant

Monisha

Prakash

Saravanan

et al. 2021

Preprint

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Vetiver (Chrysopogon zizanioides) is an essential oil-producing plant that has tremendous application in cosmetics, perfumery, and herbal medicine. Natural sterility and indiscriminate harvests lead to the risk of extinction of plant species in natural habitats. Therefore, a protocol for regeneration systems via organogenesis and somatic embryogenesis using node, leaf, and root explants has been standardized. The highest shoot regeneration frequency (72.2%) through organogenesis was attained from node explants on MS (Murashige & Skoog) medium comprising 2.0 mg L-1 BAP (“6-benzylaminopurine”). Concurrently, leaf explants cultivated on MS medium augmented by 1.0 mg L-1, 2, 4-D (“2, 4-dichlorophenoxyacetic acid”) formed the optimal frequency (75.35%) of white friable compact (WFC) callus. However, the root explant was less responsive for WFC callus induction. Organogenic WFC callus cultivated on MS medium fortified by kinetin (1.0 mg L-1) as well as BAP (1.0 mg L-1) revealed the highest shoot regeneration efficiency (75.49%) with 48 shoots per callus. Adventitious shoots obtained from node and WFC callus of both leaf and root explants cultivated on MS medium increased by NAA (2.0 mg L-1 showed the optimal rooting of 76.97%. Concomitantly, an elevated frequency of somatic embryogenesis (52.50%) was recorded from leaf explants on MS medium using BAP (0.5 mg L-1) & 2, 4-D (1.0 mg L-1). Leaf explants were superior to node and root explants for somatic embryo initiation. The cotyledonary embryos were efficiently germinated into complete plantlets on a hormone-free MS medium. The plantlets gathered from organogenesis & somatic embryo genesis was effectively acclimatized into phenomenally similar plants. This technique may be applicable for wide-range propagation, genetic engineering, and the formation of bioactive compounds.

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Section: Hardening Of Somatic Plantssupporting

confidence: 59%

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confidence: 99%

High frequency plant regeneration of Chrysopogon zizanioides via organogenesis and somatic embryogenesis-an underutilized pharmaceutically valuable biofuel plant

Monisha

Prakash

Saravanan

et al. 2021

Preprint

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“…Hal ini dikarenakan keberadaan tunas dengan kombinasi sitokinin berupa TDZ dapat memacu sel sel untuk membentuk tunas baru yang lebih banyak. Deepak, et al, (2019) menyatakan eksplan nodus atau tunas memiliki nilai efisiensi regenerasi pertumbuhan 46.66% pada TDZ 0,1 ppm.…”

Section: Perlakuanunclassified

Respon Eksplan Batang Kentang (Solanum tuberosum L.) terhadap Perlakuan Konsentrasi Thidiazuron (TDZ) pada Media MS secara In Vitro

Saputro

Setiari

Nurchayati

et al. 2020

BAF

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Kentang (Solanum tuberosum L.) merupakan komoditas yang berpotensi dalam diversifikasi pangan, namun perbanyakan bibit dengan umbi tidak efektif untuk persediaan bibit nasional. Solusi dari permasalahan tersebut adalah perbanyakan dengan kultur jaringan melalui teknik organogenesis. Penelitian ini bertujuan untuk mengetahui dan menganalisis jenis eksplan dan konsentrasi TDZ yang tepat untuk meningkatkan respon viabilitas dan organogenesis eksplan. Metode yang digunakan adalah induksi organogenesis. Eksplan batang dipotong melintang dan diinisiasi dalam media MS dengan konsentrasi TDZ berbeda. Desain penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial 2x4. Faktor pertama adalah jenis eksplan (batang tanpa tunas atau B0 dan batang bertunas atau B1), dan faktor kedua adalah konsentrasi TDZ (0ppm atau T0, 4,5ppm atau T4,5, 6ppm atau T6 dan 7,5ppm atau T7,5). Parameter yang diamati adalah persentase eksplan steril, persentase eksplan hidup, jumlah tunas, jumlah daun dan jumlah akar. Hasil penelitian menunjukkan bahwa jenis eksplan batang bertunas cenderung meningkatkan viabilitas dan persentase eksplan hidup daripada jenis eksplan batang tanpa tunas, sedangkan peningkatan konsentrasi TDZ cenderung menurunkan viabilitas dan persentase eksplan hidup. Jenis eksplan dan konsentrasi TDZ mempengaruhi secara nyata jumlah tunas, daun dan akar. Peningkatan konsentrasi TDZ sampai 4,5ppm merupakan konsentrasi optimal dalam meningkatkan jumlah tunas dan daun. Kata kunci : Solanum tuberosum, TDZ, jenis eksplan, , viabilitas, organogenesis

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“…High auxin/cytokinin ratios are used to induce roots, whereas low ratios generally form shoots (Christianson and Warnick 1983). A number of medicinal plants respond positively to the induction of these processes (Verma et al 2011;Alonso-Herrada et al 2016;Deepak et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Effect of the genotype, explant source and culture medium in somatic embryogenesis and organogenesis in Vaccaria hispanica (Mill.) Rauschert

Bedir

Ari

Vural

et al. 2022

Plant Cell Tiss Organ Cult

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Vaccaria hispanica is an interesting species with attractive agronomic properties and a wealth of valuable bioactive compounds, potentially useful for many different purposes. Surprisingly, the number of studies focused on the development of in vitro tools for a rapid production of clonal populations is extremely limited. In the present study, two wild Turkish genotypes, previously characterized as high starch and saponin producers, are used to explore the possibilities of regenerating clonal plants through somatic embryogenesis and organogenesis. This work investigates the independent effects of genotype, type of explant and composition of the culture medium, and the interactions among them, in the growth and proliferation of calli from the explants, and the induction of somatic embryogenesis and organogenesis fron the callus surface. Some of the interactions were found significant to promote these processes. V. hispanica proved to be especially responsive for callus induction from all the explants tested. Particular explant types and combinations of plant growth regulators have been identified as especially suitable to induce the different morphogenic processes. V. hispanica is remarkably prone to produce adventitious roots, which may be a problem when trying to induce somatic embryogenesis or shoot organogenesis. However, this can be exploited to develop a convenient system for in vitro secondary metabolite production.

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Efficient plant regeneration and histological evaluations of regenerants through organogenesis and somatic embryogenesis in Spermacoce hispida L.—An underutilized medicinally important plant

Cited by 8 publications

References 42 publications

High frequency plant regeneration of Chrysopogon zizanioides via organogenesis and somatic embryogenesis-an underutilized pharmaceutically valuable biofuel plant

High frequency plant regeneration of Chrysopogon zizanioides via organogenesis and somatic embryogenesis-an underutilized pharmaceutically valuable biofuel plant

Respon Eksplan Batang Kentang (Solanum tuberosum L.) terhadap Perlakuan Konsentrasi Thidiazuron (TDZ) pada Media MS secara In Vitro

Effect of the genotype, explant source and culture medium in somatic embryogenesis and organogenesis in Vaccaria hispanica (Mill.) Rauschert

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