Efficient phosphate accumulation in the newly isolated Acinetobacter junii strain LH4

Abstract

Phosphate (PO) accumulation associated with bacteria contributes to efficient remediation of eutrophic waters and has attracted attention due to its low cost, high removal efficiency and environmental friendliness. In the present study, we isolated six strains from sludge with high concentrations of chemical oxygen demand, total nitrogen and total phosphorus levels. Among them, strain LH4 exhibited the greatest PO removal ability. Strain LH4 is typical of based on physiological, biochemical, and molecular analyses and is a PO-accumulating organism (PAO) based on toluidine blue staining. The strain grew quickly when subjected to aerobic medium after pre-incubation under anaerobic condition, with a maximum OD of 1.429 after 8 h and PO removal efficiency of 99%. Our data also indicated that this strain preferred utilizing the carbon (C) sources sodium formate and sodium acetate and the nitrogen (N) sources NHCl and (NH)SO over other compounds. To achieve optimal PO removal efficiency, a C:N ratio of 5:1, inoculation concentration of 3%, solution pH of 6, incubation temperature of 30 °C, and shaking speed of 100 rpm were recommended for strain LH4. By incubating this strain with different concentrations of PO, we calculated that its relative PO removal capacity ranged from 0.67 to 3.84 mg L h, ranking in the top three among reported PAOs. Our study provided a new PO-accumulating bacterial strain that holds promise for remediating eutrophic waters, and its potential for large-scale use warrants further investigation.

Isolation and Characterization of Phosphorus Solubilizing Bacteria With Multiple Phosphorus Sources Utilizing Capability and Their Potential for Lead Immobilization in Soil

“…It has been reported that polyphosphate regarded as high-energy compound could be hydrolyzed when phosphorus accumulating bacteria undergo undernourished conditions (Wan et al, 2017;Li and Dittrich, 2019;Zhong et al, 2019). Previous literatures have reported that some bacteria belonging to Acinetobacter genus harbor the ability to synthesize polyphosphate and could be regarded as phosphorus accumulating bacteria (Keating et al, 2016;Han et al, 2018). Therefore, we can conclude that one part of soluble phosphorus can be used for the formation of bacterial substance (e.g., DNA and RNA), and another part of soluble phosphorus can be applied for the synthesis of polyphosphate during inorganic phosphorus solubilization.…”

Monitoring Ammonium Polyphosphate (APP) Biodegradation by Acinetobacter nosocomialis D-3 Using DAPI

“…Since the 1980s, Acinetobacter has been recognized as a highly efficient bacterium for phosphorus removal and has played a significant role in wastewater treatment processes [ 33 ]. Acinetobacter ’s release of organic acids and phosphatases facilitates the breakdown of PolyP, releasing soluble phosphate [ 34 , 35 ]. Thus, Acinetobacter nosocomialis D-3 holds promise as an efficient bacterium for degrading APP and can be utilized to remedy APP-based flame retardants.…”

Molecular Evidence of Internal Carbon-Driven Partial Denitrification Annamox (PdNA) in a mainstream Pilot A-B System Coupled with Side-stream EBPR treating municipal wastewater

“…Different from the known potential partial denitrifiers ( Thauera and Halomonas ) detected in the PDN systems, our pilot system enriched the genera of Acinetobacter (17.8 ± 15.5)% and Comamonadaceae (6.7 ± 3.4%), with the very low level of conventional nitrifiers detected (< 0.2%). The species Acinetobacter junii were previously reported to exhibit the ability of efficient N- and P-removal, respectively (Han et al, 2018; Ren et al, 2014). Moreover, rather than the common chemical analysis of the overall PHA variation, we applied the single- cell Raman spectroscopy technique to accurately reveal the existing dominant microorganism (the potential Acinetobacter sp.)…”