Efficient cell surface labelling of live zebrafish embryos: wash-free fluorescence imaging for cellular dynamics tracking and nanotoxicity evaluation

Jia, Hao‐Ran; Zhu, Ya‐Xuan; Xu, Ke; Pan, Guang-Yu; Liu, Xiaoyang; Qiao, Ying; Wu, Fu‐Gen

doi:10.1039/c8sc04884c

Cited by 69 publications

(36 citation statements)

References 54 publications

(44 reference statements)

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“…Recent studies demonstrated a close correlation between zebrafish and human pigmentation [9][10][11], with many depigmenting agents identified using zebrafish embryos or larvae [12][13][14][15]. In addition, zebrafish are now used to test several types of drug delivery systems, including liposomes [16,17], micelles [18], and nanoparticles [19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Lecithin-Based Dermal Drug Delivery for Anti-Pigmentation Maize Ceramide

et al. 2020

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Ceramides have several well-known biological properties, including anti-pigmentation and anti-melanogenesis, which make them applicable for use in skincare products in cosmetics. However, the efficacy of ceramides is still limited. Dermal or transdermal drug delivery systems can enhance the anti-pigmentation properties of ceramides, although there is currently no systemic evaluation method for the efficacy of these systems. Here we prepared several types of lecithin-based emulsion of maize-derived glucosylceramide, determining PC70-ceramide (phosphatidylcholine-base) to be the safest and most effective anti-pigmentation agent using zebrafish larvae. We also demonstrated the efficacy of PC70 as a drug delivery system by showing that PC70-Nile Red (red fluorescence) promoted Nile Red accumulation in the larval bodies. In addition, PC70-ceramide suppressed melanin in mouse B16 melanoma cells compared to ceramide alone. In conclusion, we developed a lecithin-based dermal delivery method for ceramide using zebrafish larvae with implications for human clinical use.

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Section: Introductionmentioning

confidence: 99%

Lecithin-Based Dermal Drug Delivery for Anti-Pigmentation Maize Ceramide

et al. 2020

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“…Moreover, as shown in Figure A, Pent‐TMP could clearly visualize the boundary of every single cell on the surface of zebrafish, even at large magnifications (Figure B). In contrast, the other commercial PM‐specific dyes, such as DiD and Cell Mask Green, could not exhibit similar results . This may be due to their excessive lipid solubility, which could not penetrate the hydrophilic mucus layer on the surface of zebrafish .…”

Section: Methodsmentioning

confidence: 92%

“…PM imaging plays an important role in biosystems. For example, when the cells are damaged, the morphology of PM changes . Additionally, the PM morphology of erythrocytes in sickle‐cell disease is obviously different from that of people without the disease.…”

Section: Methodsmentioning

confidence: 99%

An AIE‐Based Probe for Rapid and Ultrasensitive Imaging of Plasma Membranes in Biosystems

Shi

Liu

et al. 2019

Angewandte Chemie

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The abnormality of the plasma membrane (PM) is an important biomarker for cell status and many diseases. Hence, visualizing the PM, especially in complex systems, is an emerging field in the life sciences, especially in low‐resource settings. Herein, we developed a water‐soluble PM‐specific probe utilizing electrostatic and hydrophobic interaction strategies with aggregation‐induced emission as the signal output. The probe could image the PM with many advanced features (wash‐free, ultrafast staining process, excellent PM specificity, and good biocompatibility), which were demonstrated by the PM imaging of neurons. The probe allowed for the first time the imaging of erythrocytes in the complex brain environment through a fluorescence‐based method. Moreover, the PM of the epidermal and partial view of the eyeball structure of live zebrafish are also revealed.

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“…16 Moreover, because zebrafish embryos are transparent, the use of fluorescently tagged transgenes allows the visualization of the development of the neurological systems and the monitoring of neurological disorders in a living organism. 17 The current study aimed to document the potential developmental neurotoxicity of SiO 2 NPs in vivo by taking advantage of the unique features of the zebrafish model. Specifically, the hypothesis was raised that SiO 2 NPs would lead to the underdevelopment of neurological systems in zebrafish by perturbing a specific stage in neurodevelopment.…”

Section: Introductionmentioning

confidence: 99%

<p>Low-Dose Exposure of Silica Nanoparticles Induces Neurotoxicity via Neuroactive Ligand–Receptor Interaction Signaling Pathway in Zebrafish Embryos</p>

Wei¹,

Liu²,

Liang³

et al. 2020

IJN

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Objective: Silica nanoparticles (SiO 2 NPs) have been extensively employed in biomedical field. SiO 2 NPs are primarily designed to enter the circulatory system; however, little information is available on potential adverse effects of SiO 2 NPs on the nervous system. Methods: The neurotoxicity of SiO 2 NPs at different concentrations (3, 6, 12 ng/nL) on zebrafish embryos was determined using immunofluorescence and microarray techniques, and subsequently confirmed by qRT-PCR. Results: SiO 2 NPs disrupt the axonal integrity and decrease the length of axons in Tg (NBT: EGFP) transgenic lines. The number of apoptotic cells in the brain and central nervous system of zebrafish embryos was increased in the presence of 12 ng/nL of SiO 2 NPs, but the difference did not reach statistical significance. Screening for changes in the expression of genes involved in the neuroactive ligand-receptor interaction pathway was performed by microarray and confirmed by qRT-PCR. These analyses demonstrated that SiO 2 NPs markedly downregulated genes associated with neural function (grm6a, drd1b, chrnb3b, adrb2a, grin2ab, npffr2.1, npy8br, gabrd, chrma3, gabrg3, gria3a, grm1a, adra2b, and glra3). Conclusion: The obtained results documented that SiO 2 NPs can induce developmental neurotoxicity by affecting the neuroactive ligand-receptor interaction signaling pathway. This new evidence may help to clarify the mechanism of SiO 2 NPs-mediated neurotoxicity.

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Efficient cell surface labelling of live zebrafish embryos: wash-free fluorescence imaging for cellular dynamics tracking and nanotoxicity evaluation

Abstract: We developed a red-fluorescent stain capable of achieving wash-free plasma membrane imaging in vitro and in vivo.

Cited by 69 publications

References 54 publications

Lecithin-Based Dermal Drug Delivery for Anti-Pigmentation Maize Ceramide

Lecithin-Based Dermal Drug Delivery for Anti-Pigmentation Maize Ceramide

An AIE‐Based Probe for Rapid and Ultrasensitive Imaging of Plasma Membranes in Biosystems

<p>Low-Dose Exposure of Silica Nanoparticles Induces Neurotoxicity via Neuroactive Ligand–Receptor Interaction Signaling Pathway in Zebrafish Embryos</p>

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