Efficient biomass-endopolysaccharide production from an identified wild-Serbian Ganoderma applanatum strain BGS6Ap mycelium in a controlled submerged fermentation

Balamurugan, Joshini Pillai; Supramani, Sugenendran; Usuldin, Siti Rokhiyah Ahmad; Ilham, Zul; Klaus, Anita; Ikram, Nur Kusaira Khairul; Ahmad, Rahayu; Wan‐Mohtar, Wan Abd Al Qadr Imad

doi:10.1016/j.bcab.2021.102166

Cited by 7 publications

(7 citation statements)

References 39 publications

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“…A small, compact pellet produced more biomass while a hairy starburst pellet produced higher EPS, and a slightly larger compact hairy pellet produced high biomass and EPS. This result is in agreement with those reported in the literature [8,11,17,18,23]. Small, compact pellets have closely packed mycelium, which increases their weight, whereas the hairy starburst pellets have a higher surface area for nutrient and oxygen uptake and also EPS secretion into the liquid medium, which is in agreement with [34].…”

Section: Discussionsupporting

confidence: 92%

“…A previous study conducted by Supramani [11] also showed the advantages of RSM over other optimisation methods. Another study conducted by Balamurugan [17] also used RSM in order to obtain optimised conditions for Serbian Ganoderma applanatum in 30 runs for four independent variables. Ahmad [32] conducted a study to optimise the medium components for the fermentation of Lentinus squarrosulus in just 20 runs of experiments for three independent variables, similar to [23].…”

Section: Discussionmentioning

confidence: 99%

“…The mycelium biomass and EPS were extracted using the method described by Balamurugan [17] with slight modifications to the EPS extraction. The EPS extraction was performed using cold ethanol.…”

Section: Mycelium Biomass and Exopolysaccharide (Eps) Extractionmentioning

confidence: 99%

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Performance of Biomass and Exopolysaccharide Production from the Medicinal Mushroom Ganoderma lucidum in a New Fabricated Air-L-Shaped Bioreactor (ALSB)

et al. 2023

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Conventional stirred-tank bioreactor (STR) designs are optimised for cultures of bacteria but not fungal cultures; therefore, a new Air-L-Shaped Bioreactor (ALSB) was fabricated. The ALSB was designed to eliminate the wall growth and clumping of fungal mycelium in STRs. Ganoderma lucidum was used as a fungal model and its biomass and exopolysaccharide (EPS) production were maximised by optimising the agitation rate, glucose concentration, initial pH, and aeration via response surface methodology (RSM). The ALSB system generated 7.8 g/L of biomass (biomass optimised conditions: 110 rpm, 24 g/L glucose, pH 5.6, and 3 v/v of aeration) and 4.4 g/L of EPS (EPS optimised conditions: 90 rpm, 30 g/L glucose, pH 4, and 2.5 v/v of aeration). In combination, for both optimised conditions, biomass (7.9 g/L) and EPS (4.6 g/L) were produced at 110 rpm, 30 g/L glucose, pH 4, and 3 v/v of aeration with minimal wall growth. The data prove that the ALSB is a blueprint for efficient economical fungal cultivation.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

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Performance of Biomass and Exopolysaccharide Production from the Medicinal Mushroom Ganoderma lucidum in a New Fabricated Air-L-Shaped Bioreactor (ALSB)

et al. 2023

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“…The cultured broth (filtrate) was separated from KCB with the help of a Buchner filter system. The filtrate was added with 99% (v/v) cold ethanol at a ratio of 1:4 to precipitate EPS before being stored at 4°C for 3 days using improvised method [ 30 , 31 ]. Then, the precipitated EPS was isolated by centrifugation (10000 rpm, 10 min, 25°C), where the formed pellet ( Figure 1g ) will be dried and the weight is taken once it is constant [ 32 ].…”

Section: Methodsmentioning

confidence: 99%

“…Where; Y = predicted response, = the constant coefficient, = the linear coefficient, = the quadratic coefficient, = the interaction coefficient, and = the coded values [ 30 , 33 , 34 ].…”

Section: Methodsmentioning

confidence: 99%

Performance of Malaysian kenaf Hibiscus cannabinus callus biomass and exopolysaccharide production in a novel liquid culture

‘Aizat Norhisham,

Md Saad,

Ahmad Usuldin

et al. 2023

Bioengineered

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The versatility of a well-known fibrous crop, Hibiscus cannabinus (kenaf) is still relatively new to many. Kenaf’s potential applications, which can be extended even into critical industries such as pharmaceutical and food industries, have always been overshadowed by its traditionally grown fiber. Therefore, this study aimed to venture into the biotechnological approach in reaping the benefits of kenaf through plant cell suspension culture to maximize the production of kenaf callus biomass (KCB) and exopolysaccharide (EPS), which is deemed to be more sustainable. A growth curve was established which indicates that cultivating kenaf callus in suspension culture for 22 days gives the highest KCB (9.09 ± 1.2 g/L) and EPS (1.1 ± 0.02 g/L). Using response surface methodology (RSM), it was found that sucrose concentration, agitation speed, and naphthalene acetic acid (NAA) concentration can affect the production of KCB and EPS significantly ( p < 0.05) while 2,4-dichlorophenoxy acetic acid (2,4-D) was deemed insignificant. To maximize the final yield of KCB and EPS, the final optimized variables are 50 g/L sucrose, 147.02 rpm, and 2 mg/L of NAA. To conclude, the optimized parameters for the cell suspension culture of kenaf callus serve as the blueprint for any sustainable large-scale production in the future and provide an alternative cultivating method to kenaf traditional farming.

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Ganoderma adspersum (Schulzer) Donk; Ganoderma applanatum (Pers.) Pat.; Ganoderma lucidum (Curtis) P. Karst.; Ganoderma resinaceum Boud. - GANODERMATACEAE

Gafforov,

Umar,

Ghosh

et al. 2023

Ethnobiology

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Efficient biomass-endopolysaccharide production from an identified wild-Serbian Ganoderma applanatum strain BGS6Ap mycelium in a controlled submerged fermentation

Cited by 7 publications

References 39 publications

Performance of Biomass and Exopolysaccharide Production from the Medicinal Mushroom Ganoderma lucidum in a New Fabricated Air-L-Shaped Bioreactor (ALSB)

Performance of Biomass and Exopolysaccharide Production from the Medicinal Mushroom Ganoderma lucidum in a New Fabricated Air-L-Shaped Bioreactor (ALSB)

Performance of Malaysian kenaf Hibiscus cannabinus callus biomass and exopolysaccharide production in a novel liquid culture

Ganoderma adspersum (Schulzer) Donk; Ganoderma applanatum (Pers.) Pat.; Ganoderma lucidum (Curtis) P. Karst.; Ganoderma resinaceum Boud. - GANODERMATACEAE

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