Efficient base editing in tomato using a highly expressed transient system

Yuan, Shaoze; Kawasaki, Shunsuke; Abdellatif, Islam M. Y.; Nishida, Keiji; Kondo, Akihiko; Ariizumi, Tohru; Ezura, Hiroshi; Miura, Kenji

doi:10.1007/s00299-021-02662-z

Cited by 10 publications

(9 citation statements)

References 42 publications

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“…The CRISPR-Cas system uses highly specific nucleases to create double-strand breaks (DSBs) in target sites; this approach has a promising future in crop breeding. Many editing affairs are caused by transient expression in a short time [ 110 ]. As we all known, the strict regulation of GMOs has promoted the development of plant genetic engineering techniques in which target or functional gene expression is instantaneous and foreign DNA is not integrated into the genome of host plants.…”

Section: Discussionmentioning

confidence: 99%

“…However, the efficiency is far from satisfactory even with the traditional delivery method [ 109 ]. To ensure the efficient and successful editing of desired sites, strong, constitutive, and sometimes virus promoters have often been used to boost the expression level of Cas and sgRNA [ 110 , 111 , 112 , 113 ]. These methods could be used in nanomaterial-mediated genome editing, and also in other nanomaterial-based plant genetic engineering.…”

Section: Future Prospects For Plant Genetic Engineering With Nanomate...mentioning

confidence: 99%

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The Promising Nanovectors for Gene Delivery in Plant Genome Engineering

Zhi

Zhou

Pan

et al. 2022

IJMS

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Highly efficient gene delivery systems are essential for genetic engineering in plants. Traditional delivery methods have been widely used, such as Agrobacterium-mediated transformation, polyethylene glycol (PEG)-mediated delivery, biolistic particle bombardment, and viral transfection. However, genotype dependence and other drawbacks of these techniques limit the application of genetic engineering, particularly genome editing in many crop plants. There is a great need to develop newer gene delivery vectors or methods. Recently, nanomaterials such as mesoporous silica particles (MSNs), AuNPs, carbon nanotubes (CNTs), and layer double hydroxides (LDHs), have emerged as promising vectors for the delivery of genome engineering tools (DNA, RNA, proteins, and RNPs) to plants in a species-independent manner with high efficiency. Some exciting results have been reported, such as the successful delivery of cargo genes into plants and the generation of genome stable transgenic cotton and maize plants, which have provided some new routines for genome engineering in plants. Thus, in this review, we summarized recent progress in the utilization of nanomaterials for plant genetic transformation and discussed the advantages and limitations of different methods. Furthermore, we emphasized the advantages and potential broad applications of nanomaterials in plant genome editing, which provides guidance for future applications of nanomaterials in plant genetic engineering and crop breeding.

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Section: Discussionmentioning

confidence: 99%

Section: Future Prospects For Plant Genetic Engineering With Nanomate...mentioning

confidence: 99%

The Promising Nanovectors for Gene Delivery in Plant Genome Engineering

Zhi

Zhou

Pan

et al. 2022

IJMS

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“…This method achieves relatively high genome-editing efficiency in Nicotiana benthamiana [ 112 ]. In addition, transient expression techniques have been used to improve base editing in various plants [ 113 , 114 , 115 , 116 , 117 ]. Exogenous DNA may not integrate into the plant genome, which facilitates the rapid cultivation of transgene-free plants.…”

Section: Perspectivesmentioning

confidence: 99%

CRISPR/Cas9 Technique for Temperature, Drought, and Salinity Stress Responses

Fuhrmann-Aoyagi

et al. 2022

CIMB

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Global warming and climate change have severely affected plant growth and food production. Therefore, minimizing these effects is required for sustainable crop yields. Understanding the molecular mechanisms in response to abiotic stresses and improving agricultural traits to make crops tolerant to abiotic stresses have been going on unceasingly. To generate desirable varieties of crops, traditional and molecular breeding techniques have been tried, but both approaches are time-consuming. Clustered regularly interspaced short palindromic repeat/Cas9 (CRISPR/Cas9) and transcription activator-like effector nucleases (TALENs) are genome-editing technologies that have recently attracted the attention of plant breeders for genetic modification. These technologies are powerful tools in the basic and applied sciences for understanding gene function, as well as in the field of crop breeding. In this review, we focus on the application of genome-editing systems in plants to understand gene function in response to abiotic stresses and to improve tolerance to abiotic stresses, such as temperature, drought, and salinity stresses.

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“…For genome editing in plants, increasing the expression of genome-editing components may improve efficiency (Liu et al 2019). For example, transient expression of genome editing enzymes and guide RNA (gRNA) using the Tsukuba system improved base editing in tomatoes (Yuan et al 2021). The conventional way to produce genome editing crops is to produce transgenic plants harboring genome editing enzymes; after genome editing, foreign genes are removed by segregation.…”

Section: Transient Expression System Applications Other Than Recombinant Protein Productionmentioning

confidence: 99%

Transient protein expression systems in plants and their applications

Nosaki

Hoshikawa

Ezura

et al. 2021

Plant Biotechnology

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The production of recombinant proteins is important in academic research to identify protein functions. Moreover, recombinant enzymes are used in the food and chemical industries, and high-quality proteins are required for diagnostic, therapeutic, and pharmaceutical applications. Though many recombinant proteins are produced by microbial or mammalian cell-based expression systems, plants have been promoted as alternative, cost-effective, scalable, safe, and sustainable expression systems. The development and improvement of transient expression systems have significantly reduced the period of protein production and increased the yield of recombinant proteins in plants. In this review, we consider the importance of plant-based expression systems for recombinant protein production and as genetic engineering tools.

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Efficient base editing in tomato using a highly expressed transient system

Cited by 10 publications

References 42 publications

The Promising Nanovectors for Gene Delivery in Plant Genome Engineering

The Promising Nanovectors for Gene Delivery in Plant Genome Engineering

CRISPR/Cas9 Technique for Temperature, Drought, and Salinity Stress Responses

Transient protein expression systems in plants and their applications

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