Efficiency of the Bioverm® (Duddingtonia flagrans) fungal formulation to control in vivo and in vitro of Haemonchus contortus and Strongyloides papillosus in sheep

Braga, Fábio Ribeiro; Ferraz, Carolina Magri; Silva, Edir Nepomuceno da; Araújo, Jackson Víctor de

doi:10.1007/s13205-019-2042-8

Cited by 36 publications

(24 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There are few investments in the discovery of new drugs for the treatment of parasites. Considering that there are reports in the literature relating nemathophagous fungi extracts with nematicidal activity and commercial products using nemathophagous fungi [7,18,19], we performed extraction of the P. chlamydosporia metabolites with an organic solvent; the extract was fractionated with solvents of different polarity scale and evaluated the compounds for nematicidal capacity. We observed that EtOAc was able to paralyze 84% of the C. elegans larvae at a concentration of 1000 μg/ml, whereas CH 2 Cl 2 and MeOH did not show any relevant activity at the same concentration, which was probably because of the profile and quantity of substances present in EtOAc.…”

Section: Discussionmentioning

confidence: 99%

Ketamine can be produced by Pochonia chlamydosporia: an old molecule and a new anthelmintic?

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background Infection by nematodes is a problem for human health, livestock, and agriculture, as it causes deficits in host health, increases production costs, and incurs a reduced food supply. The control of these parasites is usually done using anthelmintics, which, in most cases, have not been fully effective. Therefore, the search for new molecules with anthelmintic potential is necessary. Methods In the present study, we isolated and characterized molecules from the nematophagous fungus Pochonia chlamydosporia and tested these compounds on three nematodes: Caenorhabditis elegans; Ancylostoma ceylanicum; and Ascaris suum. Results The ethyl acetate extract showed nematicidal activity on the nematode model C. elegans. We identified the major substance present in two sub-fractions of this extract as ketamine. Then, we tested this compound on C. elegans and the parasites A. ceylanicum and A. suum using hamsters and mice as hosts, respectively. We did not find a difference between the animal groups when considering the number of worms recovered from the intestines of animals treated with ketamine (6 mg) and albendazole (P > 0.05). The parasite burden of larvae recovered from the lungs of mice treated with ketamine was similar to those treated with ivermectin. Conclusions The results presented here demonstrate the nematicidal activity of ketamine in vitro and in vivo, thus confirming the nematicidal potential of the molecule present in the fungus P. chlamydosporia may consist of a new method of controlling parasites.

show abstract

Section: Discussionmentioning

confidence: 99%

Ketamine can be produced by Pochonia chlamydosporia: an old molecule and a new anthelmintic?

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…There are few investments in the discovery of new drugs for the treatment of parasites. Considering that there are works in the literature relating nemathophagous fungi extracts with nematicidal activity and commercial products using nemathophagous fungi [7,18,19], we performed extraction of the P. chlamydosporia metabolites with an organic solvent; the extract was fractionated with solvents of different polarity scale and evaluated the compounds for nematicidal capacity. We observed that EtOAc was able to paralyze 84% of the C. elegans larvae at a concentration of 1,000 μg/mL, whereas CH 2 Cl 2…”

Section: Discussionmentioning

confidence: 99%

Ketamine can be produced by Pochonia chlamydosporia: an old molecule and a new anthelmintic?

Ferreira

Machado

Furtado

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Infection by nematodes is a problem for human health, livestock, and agriculture, as it causes deficits in host health, increases production costs, and incurs less food supply. The control of these parasites is usually done using anthelmintics, which, in most cases, have not been fully effective. Therefore, the search for new molecules with anthelmintic potential is necessary. Methods: In the present study, we isolated and characterized molecules from the nematophagous fungus Pochonia chlamydosporia and tested these compounds on three nematodes: Caenorhabditis elegans, Ancylostoma ceylanicum, and Ascaris suum. Results: The ethyl acetate extract showed nematicidal activity on the nematode model C. elegans. We identified the major substance present in two subfractions of this extract as ketamine. Then, we tested this compound on C. elegans and the parasites A. ceylanicum and A. suum using hamsters and mice as hosts, respectively. We did not find a difference between the animal groups when considering the number of worms recovered from the intestines of animals treated with ketamine (6 mg) and albendazole (P > 0.05). The parasite burden of larvae recovered from the lungs of mice treated with ketamine was similar to those treated with ivermectin. Conclusion: These results presented here demonstrated the nematicidal activity of ketamine in vitro and in vivo. Thus, confirming the nematicidal potential of the molecule present in the fungus P. chlamydosporia may consist of a new method of controlling parasites.

show abstract

“…There are few investments in the discovery of new drugs for the treatment of parasites. Considering that there are reports in the literature relating nemathophagous fungi extracts with nematicidal activity and commercial products using nemathophagous fungi [7,18,19], we performed extraction of the P. chlamydosporia metabolites with an organic solvent; the extract was fractionated with solvents of different polarity scale and evaluated the compounds for nematicidal capacity. We observed that EtOAc was able to paralyze 84% of the C. elegans larvae at a concentration of 1000 μg/ml, whereas CH 2 Cl 2 and MeOH did not show any relevant activity at the same concentration, which was probably because of the pro le and quantity of substances present in EtOAc.…”

Section: Discussionmentioning

confidence: 99%

Ketamine can be produced by Pochonia chlamydosporia: an old molecule and a new anthelmintic?

Ferreira

Machado

Furtado

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Infection by nematodes is a problem for human health, livestock, and agriculture, as it causes deficits in host health, increases production costs, and incurs a reduced food supply. The control of these parasites is usually done using anthelmintics, which, in most cases, have not been fully effective. Therefore, the search for new molecules with anthelmintic potential is necessary. Methods: In the present study, we isolated and characterized molecules from the nematophagous fungus Pochonia chlamydosporia and tested these compounds on three nematodes: Caenorhabditis elegans; Ancylostoma ceylanicum; and Ascaris suum.Results: The ethyl acetate extract showed nematicidal activity on the nematode model C. elegans. We identified the major substance present in two sub-fractions of this extract as ketamine. Then, we tested this compound on C. elegans and the parasites A. ceylanicum and A. suum using hamsters and mice as hosts, respectively. We did not find a difference between the animal groups when considering the number of worms recovered from the intestines of animals treated with ketamine (6 mg) and albendazole (P > 0.05). The parasite burden of larvae recovered from the lungs of mice treated with ketamine was similar to those treated with ivermectin. Conclusions: The results presented here demonstrate the nematicidal activity of ketamine in vitro and in vivo, thus confirming the nematicidal potential of the molecule present in the fungus P. chlamydosporia may consist of a new method of controlling parasites.

show abstract

Efficiency of the Bioverm® (Duddingtonia flagrans) fungal formulation to control in vivo and in vitro of Haemonchus contortus and Strongyloides papillosus in sheep

Cited by 36 publications

References 25 publications

Ketamine can be produced by Pochonia chlamydosporia: an old molecule and a new anthelmintic?

Ketamine can be produced by Pochonia chlamydosporia: an old molecule and a new anthelmintic?

Ketamine can be produced by Pochonia chlamydosporia: an old molecule and a new anthelmintic?

Ketamine can be produced by Pochonia chlamydosporia: an old molecule and a new anthelmintic?

Contact Info

Product

Resources

About