Efficiency of 2‐phenoxyethanol and Clove Oil for Reducing Handling Stress in Reared Meagre, Argyrosomus regius (Pisces: Sciaenidae)

Barata, Marisa; Soares, Florbela; Aragão, Cláudia; Almeida, Ana Catarina; Pousão-Ferreira, Pedro; Ribeiro, Laura

doi:10.1111/jwas.12245

Cited by 36 publications

(28 citation statements)

References 23 publications

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“…There are several studies which try to determine the effective concentrations of clove oil and 2-phenoxyethanol in S. aurata and D. labrax (Mylonas et al, 2005), S. senegalensis (Weber et al, 2009), A. persicus (Adel et al, 2016), A. regius (Barata et al, 2016). However, there is no anesthetic efficacy study in doctor fish regarding these anesthetic drugs.…”

Section: Discussionmentioning

confidence: 99%

“…As reported by Mylonas et al (2005), we found that clove oil was effective at 4-fold lower concentrations than 2-phenoxyethanol on doctor fish. Barata et al (2016) explained this situation that clove oil affects different type of receptors might justify a higher efficiency and the use of lower concentrations. It is clear that the clove oil is advantageous because of its plant origin, safe nature, low price, and its effectiveness even at lower concentrations (Mylonas et al, 2005;Javahery et al, 2012;Kizak et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, culture of this fish has become a significant economic gain worldwide. It is emphasized that during the aquaculture activities, the use of anesthetic agents is required to maximize fish welfare during handling process (Barata et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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ANESTHETIC EFFICACY OF CLOVE OIL AND 2-PHENOXYETHANOL ON DOCTOR FISH, Garra rufa (HECKEL, 1843)

Aydın¹,

Akhan²,

Gümüş³

et al. 2019

Bol. Inst. Pesca

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This study aimed to determine the anesthetic efficacy of clove oil and 2-phenoxyethanol on doctor fish (Garra rufa) at two different water temperatures. Experimental fish (1.2 ± 0.2 g mean weight) were subjected to 25, 50, 75 and 100 μL L-1 clove oil and 100, 200, 300, 400 and 500 μL L-1 2-phenoxyethanol concentrations at water temperature of 15 and 25 °C, and the induction and recovery times were investigated. Results showed that induction and recovery times in doctor fish were significantly affected by clove oil and 2-phenoxyethanol concentrations as well as water temperature. The interaction of anesthetic concentration and water temperature on all induction stage time was significant in clove oil. Between the anesthetic concentration and temperature interaction was significant for recovery times in both anesthetic agents. The induction time decreased significantly with increasing concentration of both anesthetic agents at water temperature of 15 and 25 °C. The lowest effective concentrations that produced induction within 3 min and recovery within 5 min were 50-75 μL L-1 of clove oil and 300 μL L-1 of 2-phenoxyethanol in both 15 and 25 °C respectively. The results also indicated that clove oil was effective at 4-fold lower concentrations than 2-phenoxyethanol, but the recovery time was longer than 2-phenoxyethanol. These results suggest that clove oil and 2-phenoxyethanol were effective anesthetics and could be used as anesthetic agents in doctor fish.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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ANESTHETIC EFFICACY OF CLOVE OIL AND 2-PHENOXYETHANOL ON DOCTOR FISH, Garra rufa (HECKEL, 1843)

Aydın¹,

Akhan²,

Gümüş³

et al. 2019

Bol. Inst. Pesca

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“…After sedation with 800 ppm of 2-phenoxyethanol [64], blood was withdrawn from the caudal vein with a 1 mL heparinized syringe. Collection of blood samples (1 mL of blood per fish, on average) was completed within 3 min after capturing the fish to minimize the effects of handling on stress parameters.…”

Section: Sampling Protocolmentioning

confidence: 99%

Influence of Age on Stress Responses of White Seabream to Amyloodiniosis

Moreira

Cordeiro-Silva

Barata

et al. 2019

Fishes

Self Cite

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Amyloodiniosis is a disease that represents a major bottleneck for semi-intensive aquaculture, especially in Southern Europe. The inefficacy of many of the treatments for this disease on marine fish produced in semi-intensive aquaculture has led to a new welfare approach to amyloodiniosis. There is already some knowledge of several welfare issues that lead to amyloodiniosis as well as the stress, physiological, and immunological responses to the parasite by the host, but no work is available about the influence of fish age on the progression of amyloodiniosis. The objective of this work was to determine if stress, hematological, and histopathological responses are age dependent. For that purpose, we determined the mortality rate, histopathological lesions, hematological indexes, and stress responses (cortisol, glucose, lactate, and total protein) in “Small” (total weight: 50 ± 5.1 g, age: 273 days after eclosion (DAE)) and “Big” (total weight: 101.3 ± 10.4 g, age: 571 DAE) white seabream (Diplodus sargus) subjected to an Amyloodinium ocellatum infestation (8000 dinospores mL−1) during a 24-h period. The results demonstrated a strong stress response to A. ocellatum, with marked differences in histopathological alterations, glucose levels, and some hematological indexes between the fish of the two treatments. This work elucidates the need to take in account the size and age of the fish in the development and establishment of adequate mitigating measures and treatment protocols for amyloodiniosis.

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“…Under such circumstances, the use of anesthetic agents may be beneficial or even necessary. A number of chemical anesthetics have been used in fishery research, for example, MS-222, clove oil, benzocaine, and 2-phenoxyethanol (Heo and Shin, 2010) (Popovic et al, 2012; Barata et al, 2016). The most commonly used anesthetic in aquaculture practice and research is MS-222 (Popovic et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Effects of the anesthetic MS-222 on silver pomfret (Pampus argenteus) juveniles under aquaculture treatment stresses

Cao

Wang

et al. 2018

Preprint

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2The silver pomfret (Pampus argenteus) is a major economically important marine fish in China. 3However, P. argenteus is sensitive to many stress factors and susceptible to injury. This problem could 2 4 be resolved using anesthesia. We determined the lowest effective dose (LED) of tricaine 2 5 methanesulfonate (MS-222) and assessed the longest safe deep anesthesia time and effect after 2 6 aquaculture treatment stresses. P. argenteus juveniles were exposed to six concentrations of MS-222 2 7(10, 25, 50, 75, 100, and 125 mg L -1 ); LED was established at 75 mg L -1 . The juveniles were exposed 2 8to different deep anesthesia times (4, 7, 10, 12, and 15 min) at 75 mg L -1 ; the longest safe deep 2 9anesthesia time under LED was 10 min. Finally, the juveniles were randomly divided into four groups: 3 0 control group (CG), draining group (DG, drain), anesthetic group (AG, drain + MS-222 + aquaculture 3 1 treatment); and non-anesthetic group (NAG, drain + aquaculture treatment). Plasma cortisol levels in 3 2 the NAG, AG, DG, and CG groups were 38.739 ± 1.065 (highest), 25.083 ± 0.587, 28.644 ± 0.612, and 3 322.620 ± 0.836 ng mL -1 (lowest). The AG group showed significant differences in superoxide 3 4 dismutase, catalase, and malondialdehyde activities, except for glutathione. HSP70, HSP90, GR1, and 3 5GR2 mRNA levels in the NAG group increased sharply in response to stressors. GR1 and GR2 mRNA 3 6 levels in the AG group also increased significantly, whereas HSP70 and HSP90 mRNA levels showed 3 7 no significant differences. Thus, MS-222 can reduce oxidative damage, stress reaction, and resistance 3 8to aquaculture treatment stresses in P. argenteus.

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Efficiency of 2‐phenoxyethanol and Clove Oil for Reducing Handling Stress in Reared Meagre, Argyrosomus regius (Pisces: Sciaenidae)

Cited by 36 publications

References 23 publications

ANESTHETIC EFFICACY OF CLOVE OIL AND 2-PHENOXYETHANOL ON DOCTOR FISH, <i>Garra rufa</i> (HECKEL, 1843)

ANESTHETIC EFFICACY OF CLOVE OIL AND 2-PHENOXYETHANOL ON DOCTOR FISH, <i>Garra rufa</i> (HECKEL, 1843)

Influence of Age on Stress Responses of White Seabream to Amyloodiniosis

Effects of the anesthetic MS-222 on silver pomfret (Pampus argenteus) juveniles under aquaculture treatment stresses

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