Efficacy of tannins from Mimosa pudica and tannic acid in neutralizing cobra (Naja kaouthia) venom

Abstract

Abstract:In the present study, the effectiveness of Mimosa pudica tannins (MPT) in neutralizing the lethality of Naja kaouthia venom was compared with commercially derived tannins. Preincubation of MPT with N. kaouthia venom maintained 100% survival of mice after 24 hours. The mouse group in which there was no preincubation, no protection against the effects of the venom was observed. M. pudica tannin was found to be more effective in neutralizing the lethality of N. kaouthia venom when compared to commercial tannic acid. Two protein spots were missing in the two-dimensional gel electrophoresis (2-DE) of the MPT treated mouse indicating the down-regulation of venom proteins. The results from this study indicated that tannins obtained from M. pudica are better than tannic acid in neutralizing the lethality of N. kaouthia venom in vitro. However, further investigations are required to establish that M. pudica has potential for treating N. kaouthia snakebites.

Efficacy evaluations of Mimosa pudica tannin isolate (MPT) for its anti-ophidian properties

“…This means that at dye-protein binding of 100%, all the venom proteins were precipitated/bound to MPT in the sample mixture. The same binding ability was observed in previous studies by Vejayan et al (2007) and Sia et al (2011) in a double-immunodiffusion study. Both the studies showed binding capabilities between MPT and venom alongside the formation of a venom sample interaction precipitin line (Vejayan et al, 2007;Sia et al, 2011).…”

“…In this study, attempts to further evaluate the efficacy of the Mimosa pudica tannin isolate were undertaken to determine how far the plant can be used successfully as an anti-ophidian, as reported by Vejayan et al (2007) and Sia et al (2011). The study focused on tannin fraction of this plant as it has been established previously no activity was obtained by the fraction containing without any tannin (eluted with 50% methanol of the crude water extract in Sephadex LH-20 packed column) (Sia et al, 2011).…”

“…The study focused on tannin fraction of this plant as it has been established previously no activity was obtained by the fraction containing without any tannin (eluted with 50% methanol of the crude water extract in Sephadex LH-20 packed column) (Sia et al, 2011). A number of studies have reported the use tannins derived from plants against snake venom (Okonogi et al, 1979;Pithayanukul et al, 2005;Jaiswal et al, 2011).…”

Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth

“…The antiophidian ability of P. reticulata extract was attributed to the presence of tannins, which act by precipitating venom proteins, also reproduced by commercial tannic acid. Both tannins and tannic acid significantly inhibit not only the impairment of muscle strength induced by Crotalus durissus terrificus and Bothrops jararacussu venoms, but also the lethality of cobra (Naja kaouthia) venom (8,33). This protein-precipitating property of P. reticulata extract could limit its clinical use as a topical application -mainly against the local manifestation in cases of Bothrops snakebites due to high myotoxic action, but strategies for producing a hemoperfuser containing tannic acid and/or plant tannins as adsorbent could be considered for treating severe snake envenomation.…”

Inhibition of the principal enzymatic and biological effects of the crude venom of Bothrops atrox by plant extracts

“…Hydrolizable tannins were detected in all the extracts. These tannins appear to be able to ligate to the proteins found in venom during the incubation period in an unspecified fashion (Ambikabothy et al, 2011;Sia et al, 2011), which may account for the high efficiency of inhibition recorded in both the in vivo and in vitro assays run in the present study. Farrapo et al (2011) conducted a similar study with P. reticulata, one of the target species of this study, showing that the content of polyphenols tannin was 20 times higher than the content of flavonoids (0.16%), concluding that the presence of tannins in P. reticulata was able to inhibit the toxic effects of the venom of B. jararacussu.…”