Efficacy of some neutralizers in suspension tests determining the activity of disinfectants

Espigares, Elena; Bueno, Antonio; Fernández-Crehuet, Milagros; Espigares, M.

doi:10.1016/s0195-6701(03)00238-x

Cited by 37 publications

(23 citation statements)

References 4 publications

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“…According to the obtained results (Table I), polysorbate 80 was classified as inactive against the tested microorganisms. Our findings are in agreement with literature data, where polysorbate 80 was used as a neutralizer and applied in concentrations 3-6.5% was declared as non-toxic to the bacterial and fungal cells [8,9]. However, it was recorded that exogenously added polysorbate 80 have great potential to enhance or disrupt natural behaviour of some microorganisms [10][11][12].…”

Section: Antimicrobial Activity Of Essential Oils and Polysorbate 80 supporting

confidence: 92%

Lavender, tea tree and lemon oils as antimicrobials in washing liquids and soft body balms

Kunicka-Styczyńska

Sikora

Kalemba

2011

Intern J of Cosmetic Sci

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The aim of this study was to evaluate the antimicrobial activity of commercial essential oils: lavender, tea tree and lemon, antimicrobials in washing liquid and O/W soft body balm. The inhibition efficacy of essential oils in washing liquid (1% alone or in mixtures), in soft body balm (0.5% alone), as well as combined with the synthetic preservative DMDM hydantoin and 3-iodo-2-propynyl butyl carbamate mixture (0.1 and 0.3%), was tested against S. aureus ATCC 6538, P. aeruginosa ATCC 9027, Candida sp. ŁOCK 0008 and A. niger ATCC 16404 in compliance with the European Pharmacopoeia standards. The components of the system preserving soft body balm were supplemented with a solubilizer. Washing liquids containing only essential oils met Criterion A E.P. only for S. aureus, Candida sp. and A. niger. In soft body balm formulations, oils at a concentration of 0.5% did not reveal any preserving activity. The introduction of a solubilizer to a system containing 0.5% tea tree oil led to a substantial increase in the bacteriostatic activity of the formulation, but did not significantly affect its fungistatic properties. A combination of 0.5% tea tree oil, 5% solubilizer and 0.3% synthetic preservative ensured the microbiological stability of soft body balm in accordance with Criterion A E.P.

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Section: Antimicrobial Activity Of Essential Oils and Polysorbate 80 supporting

confidence: 92%

Lavender, tea tree and lemon oils as antimicrobials in washing liquids and soft body balms

Kunicka-Styczyńska

Sikora

Kalemba

2011

Intern J of Cosmetic Sci

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“…The disinfectants and antiseptics studied were as follows (at recommended concentration): glutaraldehyde, 20 mg/mL (2%); peracetic acid, 16.2 mg/mL (1.62%); chlorhexidine, 5 mg/mL (0.5%); benzalkonium chloride, 2.5 mg/mL (0.25%); povidone iodine, 25 mg/mL (2.5%); and silver nitrate, 100 mg/mL (10%). All the assays were carried out according to the norm UNE‐EN 1040:2006, as described previously (Espigares et al., ; Fernández‐Crehuet et al., ) adapting the volumes for use in a microtitre plate with 96 wells. All tests were carried out in triplicate, starting with homogeneous suspensions of 1–3 × 10 8 cfu/mL.…”

Section: Methodsmentioning

confidence: 99%

Phenotypic Resistance to Disinfectants and Antibiotics in Methicillin‐Resistant Staphylococcus aureus Strains Isolated from Pigs

Espigares

Roldán

Espigares

et al. 2016

Zoonoses and Public Health

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The aim of this research was to study the phenotypic resistances to disinfectants and antibiotics in strains of methicillin-resistant Staphylococcus aureus (MRSA) obtained from Canary black pigs. Analyses were performed on 54 strains of MRSA, isolated in Canary black pigs from the province of Tenerife (Spain); all of them carried the mecA gene. The strains were isolated by means of nasal swab samples of healthy pigs, collected under veterinarian supervision. Bactericidal activity of antiseptics and disinfectants was tested by means of the dilution-neutralization method. Susceptibility to the disinfectants glutaraldehyde, peracetic acid and silver nitrate was assessed, as well as to the antiseptics chlorhexidine, benzalkonium chloride and povidone iodine. Susceptibility to a wide array of antibiotics representing the main groups was determined by means of the disc diffusion method. All the strains demonstrated susceptibility to the disinfectants tested at the recommended concentration, and even to dilutions equal to or lesser than 1/16. The most effective antiseptic and disinfectant were, respectively, chlorhexidine and silver nitrate. With regard to the antibiotics, the strains proved to be multiresistant. All presented phenotypic resistance to the β-lactam antibiotics ampicillin, penicillin and cefoxitin, as well as to numerous aminoglycosides, tetracycline and trimethoprim-sulfamethoxazole. It was also observed that 61.1% of the strains were carriers of plasmids. Our results underline that in the strains such as MRSA, which show multiple resistances to antibiotics, the antiseptics and disinfectants show great efficacy. Moreover, as other authors also suggest, for the treatment and prevention of infections caused by MRSA, the use of β-lactam and aminoglycoside antibiotics may be less effective.

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“…The present study was conducted to evaluate different systems for neutralizing the activity of selected organic acids and commercial sanitizers. Some of these systems have been used in other biological research projects (7,10,15,23).…”

Section: Discussionmentioning

confidence: 99%

Mitigating the Antimicrobial Activities of Selected Organic Acids and Commercial Sanitizers with Various Neutralizing Agents

Park

Chen

2011

Journal of Food Protection

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This study was conducted to evaluate the abilities of five neutralizing agents, Dey-Engley (DE) neutralizing broth (single or double strength), morpholinepropanesulfonic acid (MOPS) buffer, phosphate-buffered saline (PBS), and sodium thiosulfate buffer, in mitigating the activities of acetic or lactic acid (2%) and an alkaline or acidic sanitizer (a manufacturer-recommended concentration) againt the cells of Shiga toxin-producing Escherichia coli (STEC; n = 9). To evaluate the possible toxicity of the neutralizing agents to the STEC cells, each STEC strain was exposed to each of the neutralizing agents at room temperature for 10 min. Neutralizing efficacy was evaluated by placing each STEC strain in a mixture of sanitizer and neutralizer under the same conditions. The neutralizing agents had no detectable toxic effect on the STEC strains. PBS was least effective for neutralizing the activity of selected organic acids and sanitizers. Single-strength DE and sodium thiosulfate neutralized the activity of both acetic and lactic acids. MOPS buffer neutralized the activity of acetic acid and lactic acid against six and five STEC strains, respectively. All neutralizing agents, except double-strength DE broth, had a limited neutralizing effect on the activity of the commercial sanitizers used in the study. The double-strength DE broth effectively neutralized the activity of the two commercial sanitizers with no detectable toxic effects on STEC cells.

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Efficacy of some neutralizers in suspension tests determining the activity of disinfectants

Cited by 37 publications

References 4 publications

Lavender, tea tree and lemon oils as antimicrobials in washing liquids and soft body balms

Lavender, tea tree and lemon oils as antimicrobials in washing liquids and soft body balms

Phenotypic Resistance to Disinfectants and Antibiotics in Methicillin‐Resistant Staphylococcus aureus Strains Isolated from Pigs

Mitigating the Antimicrobial Activities of Selected Organic Acids and Commercial Sanitizers with Various Neutralizing Agents

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