EFFICACY OF SEVERAL QTL AND miRNA-SSR MARKERS TOWARDS HEALTH BENEFIT TRAITS IN AN ELITE DARJEELING TEA CULTIVAR

Abstract

Antioxidant and free radical scavenging ability of tea polyphenols are considered as health benefit traits that mainly contributed by tea catechins. Therefore understanding of genetic and molecular basis of this secondary metabolite production is of prime interest to facilitate the crop improvement program in tea plant efficiently. Some QTLs for these quality traits have already been available. In present study, 10 QTL linked SSRs and 14 newly developed microRNA based SSRs have been characterized in two Darjeeling tea cultivars having contrast characters and their progeny clone TS569 for validating possible association with catechin contents and antioxidant activities. Individual catechins and radical scavenging activity have been estimated by high performance liquid chromatography and UV-Vis spectrophotometry. Some of the polymorphic loci are indicated to be co-varied with such biochemical physiognomies. Characterization of microRNA based SSRs polymorphism are being the first time report in tea and discussed as ideal choice for further genotypic studies in tea.

Genome-wide SNP discovery from Darjeeling tea cultivars - their functional impacts and application toward population structure and trait associations

“…However, the same members had shown admixture between two different groups at varying degrees when K = 3 was considered. Both analyses revealed the genetic background of TS-569 cultivar to be admixed between two separate ancestries, which is following its true biparental origin (AV-2 × T-78) (Hazra et al, 2020b).…”

“…A total of twenty-three elite tea cultivars released for plantation at the various slopes of Darjeeling hills were chosen for this study. All these accessions are being maintained at Darjeeling Tea Research and Development Center (DTRDC), Kurseong, with uniform agroecological conditions for research and breeding purposes (Hazra et al, 2020a;Hazra et al, 2020b). Most of the cultivars are commercial hybrid, generated through preferential breeding techniques among the wild and domesticated types of tea lineages.…”

Ecophysiological traits differentially modulate secondary metabolite accumulation and antioxidant properties of tea plant [Camellia sinensis (L.) O. Kuntze]

“…For determination of catechin derivatives, high-performance liquid chromatography was carried out in a Waters Alliance MDLC Separations module and dual absorbance detector (Waters e2695-2487) equipped with a C18 reverse-phase silica-based column (X-Tera) with 4.6 × 250 mm dimension at 35 °C. The double gradient elution system was followed using standardized methodology 16 . Standard reference materials of various catechin derivatives were used (Sigma-Aldrich, stored at − 20 °C and diluted from the stock for using as working solution).…”