Efficacy of lipase from Aspergillus niger as an additive in detergent formulations: a statistical approach

Abstract

The efficacy of lipase from Aspergillus niger MTCC 2594 as an additive in laundry detergent formulations was assessed using response surface methodology (RSM). A five-level four-factorial central composite design was chosen to explain the washing protocol with four critical factors, viz. detergent concentration, lipase concentration, buffer pH and washing temperature. The model suggested that all the factors chosen had a significant impact on oil removal and the optimal conditions for the removal of olive oil from cotton fabric were 1.0% detergent, 75 U of lipase, buffer pH of 9.5 and washing temperature of 25 degrees C. Under optimal conditions, the removal of olive oil from cotton fabric was 33 and 17.1% at 25 and 49 degrees C, respectively, in the presence of lipase over treatment with detergent alone. Hence, lipase from A. niger could be effectively used as an additive in detergent formulation for the removal of triglyceride soil both in cold and warm wash conditions.

A thiol-activated lipase from Trichosporon asahii MSR 54: detergent compatibility and presoak formulation for oil removal from soiled cloth at ambient temperature

“…In absence of these properties in the present lipase an attempt was made to develop prewash formulation to clean oil stains. Majority of the literature reports are related to enzyme as detergent additives [10,11] rather than prewash formulations. However, there are reports of sequential washing regime, where lipolytic activity is carried out at low pH followed by detergent washing at alkaline pH [15].…”

lip2, a novel lipase gene cloned from Aspergillus niger exhibits enzymatic characteristics distinct from its previously identified family member

“…The lipases from Aspergillus niger have positional selectivity toward the sn-1,3 positions of the glycerol ester with substrate preference for medium-chain length fatty acids, an optimal pH between 4 and 7. They are generally regarded as safe (GRAS) extracellular enzymes by the FDA of USA and are used in various industrial fields (Schuster et al 2002;Sarıyar and Heperkan 2003;Saisubramanian et al 2006).…”

Aspergillus niger lipase: gene cloning, over-expression in Escherichia coli and in vitro refolding

“…It is also considered to be GRAS (Generally Regarded As Safe) by the FDA of the USA (Schuster et al 2002). A. niger lipase is widely used in many industrial applications (Sarıyar and Heperkan 2003;Saisubramanian et al 2006). Research on it has concentrated on production, purification, immobilization and applications.…”