Efficacy of epsilon-poly-L-lysine inhibition of postharvest blue mold in apples and potential mechanisms

Dou, Yong; Routledge, Michael N.; Gong, Yun; Godana, Esa Abiso; Dhanasekaran, Solairaj; Yang, Qiya; Zhang, Xiaoyun; Zhang, Hongyin

doi:10.1016/j.postharvbio.2020.111346

Cited by 43 publications

(19 citation statements)

References 42 publications

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“…This inhibitory effect of 3-MP was positively correlated with the concentration of 3-MP. This dose dependence is consistent with the previous studies of other compounds such as hinokitiol and epsilon-poly-L-lysine ( Dou et al, 2021 ).…”

Section: Discussionsupporting

confidence: 93%

Biocontrol Effect and Possible Mechanism of Food-Borne Sulfide 3-Methylthio-1-Propanol Against Botrytis cinerea in Postharvest Tomato

et al. 2021

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Botrytis cinerea is one of the most destructive fungal pathogens causing tremendous losses in fresh fruit or vegetables. 3-Methylthio-1-propanol (3-MP) is a naturally occurring food-borne sulfide, which is mainly used to increase the flavor in food. However, the potential application of 3-MP in the postharvest phase to manage fruit fungal diseases has not been explored. In this study, the antifungal activity of 3-MP against B. cinerea was evaluated, and the possible mechanism involved was explored. In vitro 3-MP treatment could effectively inhibit the mycelial growth, spore germination, and germ tube elongation of B. cinerea. 3-MP also impaired the spore viability and membrane integrity of B. cinerea as well as increased the leakage of nucleic acids, proteins, and malondialdehyde (MDA) in B. cinerea. In vivo 3-MP fumigation treatment inhibited the infection of B. cinerea on tomato fruits. Also, the fruits with 3-MP fumigation treatment exhibited higher antioxidant enzyme activity, lower MDA content, and a significant delay of induction of the expression of most of the stress-related genes when compared to the control group. Moreover, a cytotoxicity evaluation revealed that 3-MP had no toxicity to normal cells in a certain concentration range. Collectively, our research results will provide evidence for the development of food-borne sulfide 3-MP as a fungicide in food and agriculture and will provide an important reference for the formulation of B. cinerea biocontrol strategies.

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Section: Discussionsupporting

confidence: 93%

Biocontrol Effect and Possible Mechanism of Food-Borne Sulfide 3-Methylthio-1-Propanol Against Botrytis cinerea in Postharvest Tomato

et al. 2021

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“…Multiple reports have described that ε-PL inhibits the growth of various microorganisms by affecting the integrity and permeability of their cell walls and cell membranes ( Hyldgaard et al, 2014 ; Wei M. et al, 2018 ; Lan et al, 2019 ; Su et al, 2019 ; Liu H. et al, 2020 ; Dou et al, 2021 ). For example, in Escherichia coli O157:H7 ( Figure 6A ), the interactions between ε-PL and phospholipid groups result in distorting and shriveling of the cell membrane in a non-specific, carpet-like mechanism, in which the membrane is forced to fold in on itself, contributing to vesicle/micelle and toroidal pore formation ( Hyldgaard et al, 2014 ).…”

Section: Recent Advances In Applications Of ε-Plmentioning

confidence: 99%

Epsilon-poly-L-lysine: Recent Advances in Biomanufacturing and Applications

Wang

Zhang

et al. 2021

Front. Bioeng. Biotechnol.

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ε-poly-L-lysine (ε-PL) is a naturally occurring poly(amino acid) of varying polymerization degree, which possesses excellent antimicrobial activity and has been widely used in food and pharmaceutical industries. To provide new perspectives from recent advances, this review compares several conventional and advanced strategies for the discovery of wild strains and development of high-producing strains, including isolation and culture-based traditional methods as well as genome mining and directed evolution. We also summarize process engineering approaches for improving production, including optimization of environmental conditions and utilization of industrial waste. Then, efficient downstream purification methods are described, including their drawbacks, followed by the brief introductions of proposed antimicrobial mechanisms of ε-PL and its recent applications. Finally, we discuss persistent challenges and future perspectives for the commercialization of ε-PL.

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“…Recently, some PAAs, such as poplar bud extract and its main component, namely, pinocembrin ( Yang et al, 2016 ), citral ( Tao et al, 2014 ), limonin ( Li et al, 2021b ), salicylic acid, and plant essential oil ( Moosa et al, 2021 ), are effective in the suppression of P. italicum growth and in inducing resistance to citrus postharvest blue mold. In addition, Dou et al (2021) reported that 0.6 g/L epsilon-poly-L-lysine (ε-PL) effectively reduced BM incidence in “Fuji” apple by activating the host-defense responses to P. expansum infection. Antofine (ATF; also known as 7-demethoxytylophorine), a natural water-soluble phenanthroindolizidine alkaloid, is known to be mainly isolated from the family of Asclepiadaceae plants and exhibits excellent biological properties, such as anti-angiogenic activity ( Oh et al, 2017 ), cholinesterase inhibitory activity ( Othman et al, 2017 ), antiproliferative and antitumor activities ( Yu et al, 2016 ), and antifungal acidity against Phytopathogen Fusarium graminearum ( Mogg et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Antofine Triggers the Resistance Against Penicillium italicum in Ponkan Fruit by Driving AsA-GSH Cycle and ROS-Scavenging System

et al. 2022

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Postharvest fungal infection can accelerate the quality deterioration of Ponkan fruit and reduce its commodity value. Penicillium italicum is the causal pathogen of blue mold in harvested citrus fruits, not only causing huge fungal decay but also leading to quality deterioration. In our preliminary study, antofine (ATF) was found to have a great potential for significant in vitro suppression of P. italicum growth. However, the regulatory mechanism underpinning ATF-triggered resistance against P. italicum in citrus fruit remains unclear. Here, the protective effects of ATF treatment on blue mold development in harvested Ponkan fruit involving the enhancement of ROS-scavenging system were investigated. Results showed that ATF treatment delayed blue mold development and peel firmness loss. Moreover, the increase of electrolyte leakage, O2•– production, and malonyldialdehyde accumulation was significantly inhibited by ATF treatment. The ATF-treated Ponkan fruit maintained an elevated antioxidant capacity, as evidenced by inducted the increase in glutathione (GSH) content, delayed the declines of ascorbic acid (AsA) content and GSH/oxidized GSH ratio, and enhanced the activities of superoxide dismutase, catalase, peroxidase, and six key AsA-GSH cycle-related enzymes, along with their encoding gene expressions, thereby maintaining ROS homeostasis and reducing postharvest blue mold in harvested Ponkan fruit. Collectively, the current study revealed a control mechanism based on ATF-triggered resistance and maintenance of a higher redox state by driving AsA-GSH cycle and ROS-scavenging system in P. italicum-infected Ponkan fruit.

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Efficacy of epsilon-poly-L-lysine inhibition of postharvest blue mold in apples and potential mechanisms

Cited by 43 publications

References 42 publications

Biocontrol Effect and Possible Mechanism of Food-Borne Sulfide 3-Methylthio-1-Propanol Against Botrytis cinerea in Postharvest Tomato

Biocontrol Effect and Possible Mechanism of Food-Borne Sulfide 3-Methylthio-1-Propanol Against Botrytis cinerea in Postharvest Tomato

Epsilon-poly-L-lysine: Recent Advances in Biomanufacturing and Applications

Antofine Triggers the Resistance Against Penicillium italicum in Ponkan Fruit by Driving AsA-GSH Cycle and ROS-Scavenging System

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