Efficacy of Different Collection Techniques on Yield and Quality of Egyptian Buffalo Oocytes.

Hammad, E. M.; Gabr, Sh. A.; El‐Ratel, Ibrahim T.; Gad, Maha A.

doi:10.21608/jappmu.2014.70526

Cited by 4 publications

(5 citation statements)

References 18 publications

(24 reference statements)

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“…Results showed that; bovine oocytes maturation time is about 28-30hrs., which is correlated to the collection method in which the aspiration method yield a less oocytes count but with a predominant degree of maturation in regarding to other methods. (34) observed that; when trying to compare between different methods for oocytes collection in regarding to the Oocytes number, quality and degree of maturation, the slicing and slicing after aspiration methods came in earlier methods that yield more Oocytes count with good quality that reached to maturity condition in well considered degree. In present study we conclude that; kind of bovine oocytes collection from abattoir ovarian specimens may have an effect on oocytes count as well as maturation.…”

Section: Effect Of Invitro Maturation (Ivm) Upon Oocytes Collectionmentioning

confidence: 99%

Assessment of different methods of bovine oocytes collection, maturation and Invitro fertilization of abattoir specimens

Saleh¹

2017

IJVS

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The aim of this study is designed to evaluate the best methods for cow oocytes collection from abattoir specimens which is the cheapest, easily obtained and bulky number. Forty five fresh cow genitalia specimens and testicle were collected directly after slaughter from Al-Shoáalla abattoir northwest of Baghdad the capital early morning, transported in cool box under (4-8) °C to the laboratory of theriogenology in the college of veterinary medicine/Baghdad University during the period from November 2016 to February 2017. Ovaries were separated from the surrounding tissues, washed thoroughly with dis. water repeatedly, then with normal saline and finally with MEM medium containing Antibiotics and Nystatin for contaminant elimination. Oocytes were collected with four methods aspiration, slashing, slicing after aspiration and slicing. The result showed that; the collected oocytes were 55, 68, 87 and 106 oocytes respectively; slicing methods yield more oocytes count. Period of time between slaughtering and samples processing significantly affect oocytes collected percentage and quality, periods as 2, 6, 12 and 24 hours yield 75%, 68%, 61% and 55% oocytes counts of good, fair, poor to aged and bad quality oocytes respectively. Two hours period yield an elevated oocytes count with good quality. Maturation index of oocytes according to the type of collected methods showed 44, 37, 39 and 42 with 12, 8, 6 and 6 good oocyte quality for the four methods respectively. In conclusion slicing methods yield more oocytes count with a moderate quality and embryos production while aspiration methods yield a moderate oocytes count with an elevated quality and good embryos production.

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Section: Effect Of Invitro Maturation (Ivm) Upon Oocytes Collectionmentioning

confidence: 99%

Assessment of different methods of bovine oocytes collection, maturation and Invitro fertilization of abattoir specimens

Saleh¹

2017

IJVS

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“…Ovaries which do not possess CL have higher number of follicles being recovered and also higher grade COCs than those non CL bearing ovaries (Khandoker et al, 2011). Furthermore, the stroma of buffalo ovaries are thick and follicles are deeply embedded in them and therefore aspiration of oocytes from follicles gets difficult (Selvaraj et al, 2008, Hammad et al, 2014. The ovaries which had CL yielded lower number of oocytes as compared to non CL bearing ovary as it was discussed by (Kumar et al 1997).…”

Section: Aspiration Of Buffalo Follicular Oocytes and Recovery Percentagementioning

confidence: 99%

“…In vitro maturation is a method in which immature oocytes of dead animals are retrieved from the ovary using various methods such as aspiration, scoring, slicing, puncture or a combination of aspiration plus slicing method (Khan et al, 1997, Mehmood et al, 2011, Hammad et al, 2014 and are allowed to mature in the laboratory which is a crutial step for fertilization and subsequent embryonic development. Maturation rate in cattle and buffaloes is almost similar (94 v/s 87% respectively) but a slightly lower cleavage rate is observed in buffalo as compared to cattle (84 v/s 65%) (Gasparrini, 2002, Neglia, 2003 In vitro fertilization has drawn interest of many researchers as it can salvage the genetic potential from infertile female and can also yield large number of embryos from slaughter house derived oocytes which may go waste otherwise (Kumar 2012).…”

Section: Introductionmentioning

confidence: 99%

Cleavage Rate of in vitro Matured Oocytes Fertilized with Conventional Semen in Buffaloes

Pitroda¹,

Khillare²,

Amle³

et al. 2021

IJAR

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Background: In vitro embryo production in buffaloes has gained much importance in this current scenario due to ever increasing population and high demand of milk and meat. Slaughter house derived bubaline ovaries are a cheap and abundant source of cumulus oocyte complexes.Methods: Oocytes from the buffalo ovarian follicles were recovered by aspiration technique as it facilitates quick recovery. Total 155 ovaries were used in the present study. Surface follicles were measured using vernier calliper and categorized into three groups viz. less than 3 mm, 3-5 mm and greater than 5 mm based on follicular diameter and oocytes were processed for IVM, IVF and IVC using conventional non sorted semen.Result: Overall percentage of small, medium and large follicles in the ovaries were recorded as 16.29 ± 0.94%, 8.14±0.60%, 5.35 ± 0.76%, respectively. Overall recovery rate of COCs was 38%. The percentage of these oocytes were 16.74% (A), 15.25% (B), 25.26% (C), 18.33% (D) and 29.87% (E) respectively. Maturation rate of oocytes were 81.96 ± 2.70%. Fertilization rate was 74.98 ± 3.87%, Cleavage rate % was 40.84±2.51% and Blastocyst percentage was 21.57±1.75% respectively. Application of in vitro embryo production technique using slaughter house ovaries can salvage the genetic potential of bubaline species.

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“…According to Hammad et al (2016) when trying to compare between different methods for oocytes collection in regarding to the oocytes number, quality and degree of maturation, the slicing and slicing after aspiration methods came in earlier methods that yield more oocytes count with good quality that reached to maturity condition in well considered degree. The oocytes collection methods also did not influence subsequent embryonic developmental competence after in vitro fertilization with M II stage oocytes (Wang et al, 2007).…”

Section: The Effect Of Collection Technique On Ivmmentioning

confidence: 99%

Evaluation of Different Techniques in Recovering of Oocytes and Storage Duration of Ovaries on the Quality and Quantity of Bovine in Vitro Maturation

et al. 2020

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This study aimed to evaluate different recovery techniques of oocytes and the duration time between ovary collection and processing in the laboratory on the quality and quantity of Simmental cross bovine oocytes in vitro maturation. A total of 75 bovine ovaries were divided into three groups. Experiment-1; the storage duration of ovaries were 6, 9 and 12 h. Experiment -2; the techniques for oocytes recovery were slicing, aspiration and slicing + aspiration. The ovaries were collected from slaughterhouse and kept in saline solution during transportation to laboratory. A total of 136 oocytes were used for experiment-1, and 246 oocytes for experiment-2. This study found that the number and quality of oocytes were significantly different (P<0.05) as the effect of storage time. The average number of oocytes were 15.90±2.48; 17.10±1.38 and 13.60±3.00, in 6 h; 9 h and 12 h of storage time, respectively. The techniques of oocytes collection significantly (P<0.05) affect the quantity of oocytes but no significant (P>0.05) on oocytes quality. The average of oocytes of groups slicing, aspiration and slicing plus aspiration were 14.66±2.09; 9.46±2.99 and 16.40±6.86, respectively. Both experiment was significant effect (P<0.05) on immaturation rate of bovine. Conclusion of this study the storage time of ovaries was suitable in 9 h and the technique of oocytes collection was in slicing plus aspiration.

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Efficacy of Different Collection Techniques on Yield and Quality of Egyptian Buffalo Oocytes.

Cited by 4 publications

References 18 publications

Assessment of different methods of bovine oocytes collection, maturation and Invitro fertilization of abattoir specimens

Assessment of different methods of bovine oocytes collection, maturation and Invitro fertilization of abattoir specimens

Cleavage Rate of in vitro Matured Oocytes Fertilized with Conventional Semen in Buffaloes

Evaluation of Different Techniques in Recovering of Oocytes and Storage Duration of Ovaries on the Quality and Quantity of Bovine in Vitro Maturation

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