Efficacy of antioxidants in the yeast Saccharomyces cerevisiae correlates with their effects on protein thiols

Bednarska, Sabina; Leroy, Pierre; Zagulski, Marek; Bartosz, Grzegorz

doi:10.1016/j.biochi.2008.05.013

Cited by 13 publications

(11 citation statements)

References 26 publications

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“…In this assay no growth restoration was seen for typical polyphenol antioxidants, including curcumin (<0.1 mg/ml), S.tamuraet al for the action of an antioxidant (Bednarska et al 2008). Krasowska et al (2003) observed that the effect of antioxidants on the growth phenotype of SOD1Δ cells depends on growth conditions, especially oxygen supply to cells.…”

Section: Evaluation Of Antioxidants By the Growth Test Of Sod1δ Cellsmentioning

confidence: 75%

A Simple Growth Test of a Saccharomyces cerevisiae Cu, Zn-Superoxide Dismutase-Deficient Mutant in Hypertonic Medium for Biological Evaluation of Antioxidants

Tamura¹,

Wada²,

Hase³

et al. 2010

FSTR

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Osmotic stress hampers the growth of Saccharomyces cerevisiae, and this effect is mediated by oxidative stress. A simple test for the biological evaluation of antioxidants was developed on the basis of recovery of growth delay of Cu, Zn-superoxide dismutase-lacking yeast (SOD1Δ) under hyperosmosis. The SOD1Δ strain suffered from growth inhibition in a medium containing 1.7 M sorbitol. Protein oxidation in SOD1Δ cells was greatly increased by exposure to a hypertonic medium, indicating the accumulation of reactive oxygen species followed by oxidation of cellular materials. The hyperosmosis-induced growth arrest of SOD1Δ was abolished by the addition of L-ascorbic acid to the medium; the antioxidant effect depended on the concentration, ranging from 1 to 10 mM. Cysteine, N-acetyl-cysteine, and glutathione were also able to restore the growth of SOD1Δ cells. High concentrations of these thiol compounds proved less effective, probably due to adverse effects of an excess of these antioxidants. No growth restoration was seen for typical polyphenol antioxidants, including curcumin, quercetin and catechin.

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Section: Evaluation Of Antioxidants By the Growth Test Of Sod1δ Cellsmentioning

confidence: 75%

A Simple Growth Test of a Saccharomyces cerevisiae Cu, Zn-Superoxide Dismutase-Deficient Mutant in Hypertonic Medium for Biological Evaluation of Antioxidants

Tamura¹,

Wada²,

Hase³

et al. 2010

FSTR

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“…For instance, it was found that a short-term pre-treatment with quercetin increased life span and oxidative stress resistance (Belinha et al 2007). As an antioxidant, quercetin also prevented translocation of the yeast transcription factor Yap1p into the nucleus under oxidative stress conditions (Bednarska et al 2008). It was also reported that quercetin might act as a modulator of cellular signaling pathways related to carbohydrate metabolism and cell integrity to exert its protective effects against ROS in S. cerevisiae (Vilaça et al 2012).…”

Section: Introductionmentioning

confidence: 97%

Quercetin increases stress resistance in the yeast Saccharomyces cerevisiae not only as an antioxidant

2015

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Quercetin is one of the most abundant dietary flavonoids and one of the most studied antioxidants. In this work, the effect of quercetin on stress resistance of exponentially growing Saccharomyces cerevisiae cells was estimated. Pre-treatment with low concentrations (10 μM and 100 μM) of quercetin enhanced yeast viability under exposure to hydrogen peroxide, copper ions (Cu 2+ ), and heat shock. The stress-protective effects of quercetin were not connected with involvement of oxidative stress regulator Yap1p, or with induction of endogenous antioxidant defense. However, pretreatment of wild type and Δyap1 strains with quercetin prevented protein oxidation and antioxidant enzyme inactivation in yeast cells under H 2 O 2 -induced oxidative stress. Cycloheximide-an inhibitor of mRNA translation-or a deficiency in the transcriptional regulators Msn2p and Msn4p abolished the protective effects of low concentrations of quercetin against H 2 O 2 toxicity. It was shown in vitro that quercetin can scavenge H 2 O 2 and produce low amounts of H 2 O 2 in a pH-dependent manner. The antioxidant-related and antioxidant-independent mechanisms of quercetin effects are discussed.

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“…These obvious SOD1D phenotypes could be ameliorated by supplementing AsA at physiological levels, indicating the potent ability of AsA to scavenge endogenous ROS (10,13,14). Therefore, the yeast SOD1D mutant can be used as a biosensor for low molecular weight antioxidants on the basis of its ability to relieve the oxidative damage-induced phenotype (15)(16)(17)(18).…”

Section: Introductionmentioning

confidence: 99%

Ascorbic Acid and Thiol Antioxidants Suppress Spontaneous Mutagenesis in a Cu,Zn-superoxide Dismutase-deficient Mutant of Saccharomyces cerevisiae

Nagira

Tamura

Ikeda

2013

Genes and Environment

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Cu,Zn-superoxide dismutase (SOD1) is a critical enzyme in the cellular antioxidant system. The yeast Saccharomyces cerevisiae SOD1 mutant (SOD1D) exhibits a moderate mutator phenotype under aerobic conditions. The mutation frequency of a SOD1D strain determined by a CAN1 forward-mutation assay was about 12-fold higher than that of the parental strain. Base substitutions G･C→ T･A, G･C→A･T, and A･T→C･G were most commonly observed in CAN1 mutants, indicating that the mutations are caused mainly by oxidative DNA damage. The mutation frequency of SOD1D was reduced in a dose-dependent manner by cultivating it in the presence of ascorbic acid, implying that the SOD1D mutant can be used as a tester strain for small molecule antioxidants. Exogenous glutathione and N-acetylcystein also alleviated the mutator phenotype. The results indicate that ascorbic acid and thiol antioxidants are able to e‹ciently protect cells against oxidative damage-induced mutagenesis. In this assay, no apparent mutation suppression was seen for other categories of antioxidants including resveratrol, Trolox and melatonin.

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Efficacy of antioxidants in the yeast Saccharomyces cerevisiae correlates with their effects on protein thiols

Cited by 13 publications

References 26 publications

A Simple Growth Test of a Saccharomyces cerevisiae Cu, Zn-Superoxide Dismutase-Deficient Mutant in Hypertonic Medium for Biological Evaluation of Antioxidants

A Simple Growth Test of a Saccharomyces cerevisiae Cu, Zn-Superoxide Dismutase-Deficient Mutant in Hypertonic Medium for Biological Evaluation of Antioxidants

Quercetin increases stress resistance in the yeast Saccharomyces cerevisiae not only as an antioxidant

Ascorbic Acid and Thiol Antioxidants Suppress Spontaneous Mutagenesis in a Cu,Zn-superoxide Dismutase-deficient Mutant of Saccharomyces cerevisiae

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