Efficacy of a novel topical fipronil, (S)-methoprene, eprinomectin and praziquantel combination against naturally acquired intestinal nematode and cestode infections in cats

Abstract

The efficacy of a novel topical combination formulation of fipronil, (S)-methoprene, eprinomectin and praziquantel against naturally acquired intestinal nematode and cestode infections in cats was evaluated in seven negative control, blinded studies. Cats were selected based on a pre-treatment faecal examination indicating a patent infection with at least hookworms (two studies), Toxocara ascarids (one study), taeniid cestodes (two studies) or Dipylidium cestodes (two studies). In each study, cats were assigned randomly to blocks of two animals each, based on decreasing pre-treatment body weight and were randomly allocated to one of two groups of six to 12 cats: untreated (control) or treated with topical fipronil (8.3%, w/v), (S)-methoprene (10%, w/v), eprinomectin (0.4%, w/v) and praziquantel (8.3%, w/v) (BROADLINE(®), Merial) at 0.12 mL/kg body weight (providing a minimum of 10mg fipronil+12 mg S-methoprene+0.5mg eprinomectin+10mg praziquantel per kg body weight). The topical treatment was administered directly on the skin in the midline of the neck in a single spot once on Day 0. For parasite recovery and count, cats were euthanized humanely and necropsied seven or ten days after treatment. A single treatment with the novel topical combination product provided 91% efficacy against Ancylostoma braziliense, ≥ 99% efficacy against Ancylostoma tubaeforme, and >97% efficacy against Toxocara cati. Similarly, excellent efficacy was established against Taenia taeniaeformis, Dipylidium caninum and Diplopylidium spp. as demonstrated by >97% and up to 100% reductions of cestode counts in the treated cats when compared to the untreated controls (P<0.01). All cats accepted the treatment well based on health observations post-treatment and daily health observations. No adverse experiences or other health problems were observed throughout the studies. The results of this series of controlled studies demonstrated high efficacy and excellent acceptability of the novel topical combination formulation of fipronil, (S)-methoprene, eprinomectin and praziquantel against a broad range of feline intestinal nematode and cestode infections.

Pharmacokinetics of a novel endectoparasiticide topical formulation for cats, combining esafoxolaner, eprinomectin and praziquantel

“…Eprinomectin and praziquantel plasma profiles following a topical application of the combined formulation have systemic endoparasiticide concentrations needed to kill nematodes and cestodes. Both compounds also have appropriate PK parameters (AUC, C max and T max ), that are identical to those of Broadline Ò , a product with proven efficacy against nematodes and cestodes [12,29,35]. This was confirmed by efficacy data obtained with NexGard Ò Combo against both nematodes and cestodes [13] in cats.…”

“…Eprinomectin is an avermectin, from the macrocyclic lactone class, binding selectively to glutamate-gated chloride ion channels on nerves and muscular cells of several types of invertebrates, including nematodes. Eprinomectin is a well-known compound used in cattle, sheep and goats [27,33], and cats [12,29]. Praziquantel is a pyrazino-isoquinoline derivative anthelminthic that acts specifically on cestodes and trematodes, and is a well-known compound in veterinary and human medicine, in oral and topical forms [2,3,13,29,32,35,39].…”

Comparative assessment of complex drugs based on moxidectin and praziquantel for cats endoparasitic invasions

“…та інших видів гельмінтів. Ендопаразити котів значно поширені в останні два десятиліття не лише у групах високого ризику, таких як бродячі -дикі коти або коти з притулків для порятунку, а й в котів в умовах сільської місцевості та міського середовища (Knaus et al, 2014). Зазвичай ентеральні паразити викликають досить неспецифічні симптоми, такі як: блідість слизових оболонок, тьмяна шерсть, кашель, блювота, діарея, слизистий або кривавий кал, втрата апетиту, здуття живота або "пузатий" зовнішній вигляд.…”

Efficacy of two endectoparasiticide products combining fipronil and (S)-methoprene or esafoxolaner with eprinomectin and praziquantel against fleas and intestinal helminths in cats naturally infested in Brazil

“…Fipronil/ (S)-methoprene and esafoxolaner provide insecticide and acaricide properties, eprinomectin and praziquantel provide nematodicide and cestodicide properties, respectively. Broadline Ò has been used safely for several years [8] and was demonstrated to be efficacious against a broad spectrum of feline parasites, including fleas [1] and gastro-intestinal helminths [13,24]. NexGard Ò Combo was recently developed and registered in the European Union [9] and was demonstrated to be efficacious against fleas in experimental infestation studies conducted with isolates originating from France, South Africa and the USA [25], and in field studies with naturally infested cats from Europe, the USA and Australia [27], and against gastrointestinal helminths using induced and natural models of infection with isolates originating from Europe, North America and South Africa [12].…”