Effects that bovine sperm cryopreservation using two different extenders has on sperm membranes and chromatin

Celeghini, Eneiva Carla Carvalho; Arruda, Rubens Paes de; Andrade, André Furugen Cesar de; Nascimento, Juliana; Raphael, Cláudia Fernandes; Rodrigues, Paulo Henrique Mazza

doi:10.1016/j.anireprosci.2007.02.001

Cited by 162 publications

(156 citation statements)

References 25 publications

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“…In our study, E2 compared to other equilibration times has resulted in significantly higher post-thaw sperm PM, RV, VAP, VSL, VCL, STR, LIN, SV-PMI, MMP, and V-IACR. Similar to our results, some studies have reported improved post-thaw sperm STR and LIN with 4 h equilibration time in buffalo (Kumar et al, 2015) and cattle (Celeghini et al, 2008;Leite et al, 2010). Tuli et al (1981) have also reported higher sperm motility in buffalo with 4 h equilibration compared to 2 and 6 h equilibration times.…”

Section: Discussionsupporting

confidence: 81%

Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa

Shah

Andrabi²,

Qureshi

2016

Andrology

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SUMMARYThe effects of equilibration times (E1, 2 h; E2, 4 h; E3, 6 h), freezing rates (FR1, manual, 5 cm above liquid nitrogen (LN 2 ) for 10 min, plunging in LN 2 ; FR2, programmable ultra-fast, holding at +4°C for 2 min, from 4 to À10°C at À10°C/min, from À10 to À20°C at À15°C/min, from À20 to À120°C at À60°C/min, holding at À120°C for 30 sec, plunging in LN 2 ), and thawing rates (T1, 37°C for 30 sec; T2, 50°C for 15 sec; T3, 70°C for 7 sec) were evaluated on quality of buffalo bull spermatozoa. Progressive motility (%), rapid velocity (%), average path velocity (VAP, lm/s), straight line velocity (VSL, lm/s), and mitochondrial transmembrane potential (%) were higher (p < 0.05) with E2, FR2, and T3 compared to other groups. Sperm curved line velocity (VCL, lm/s) was higher (p < 0.05) with E2 and FR2 compared to other groups. Sperm straightness (%) and linearity (LIN, %) were higher (p < 0.05) with E2 compared to other groups. Sperm LIN was affected (p < 0.05) with T3 compared to other groups. Supravital-plasma membrane integrity (%), viability and acrosome integrity (%) of spermatozoa were higher (p < 0.05) with E2 and FR2 compared to other groups. Sperm DNA integrity (%) was higher (p < 0.05) with FR2 and T1 compared to other groups. We concluded that inclusion of 4 h-equilibration time, programmable ultra-fast freezing rate, and rapid thawing at 70°C for 7 sec in cryopreservation protocol improves the post-thaw quality of buffalo bull spermatozoa.

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Section: Discussionsupporting

confidence: 81%

Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa

Shah

Andrabi²,

Qureshi

2016

Andrology

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“…We can summarise that the greatest sperm activity during the entire test was for extenders containing egg yolk (P<0.05-0.01). Bioxcell was also used in the study of Celeghini et al (2008), who reported the same conclusions -that the other extenders which they evaluated were significantly better than Bioxcell. A contrary finding by Stradaioli et al (2007) that Bioxcell achieved significantly (P<0.05) better results was not confirmed in our observation.…”

Section: Extender Effect On Traits Of Sperm Activity After Thawingmentioning

confidence: 68%

“…Use of these extenders is recommended because of their excellent protection of sperm cells (Celeghini et al 2008). Nevertheless, the use of egg yolk as a cryoprotectant has recently been restricted in some countries for reasons of hygiene risk (Thun et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Effect of sire and extender on sperm motility and share of live or dead sperm in bulls’ fresh ejaculate and in AI doses after thawing

et al. 2012

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The objectives were to evaluate the effects of sire and content of fresh or ionised egg yolk in extenders on sperm motility and share of live and dead sperm in collected ejaculate, in thawed artificial insemination (AI) doses, and during thermodynamic testing compared to extenders not containing egg yolk. Ejaculates were collected once a week from 4 Holstein bulls. Each of the 20 ejaculate samples from each bull was diluted with 4 different extenders. AndroMed and Bioxcell (no egg yolk) and Triladyl and Optidyl (fresh, ionised egg yolk) were used. A total of 640 AI doses were analysed. The volume of samples, sperm concentration, and percentage of motile spermatozoa were evaluated after collection, as was sperm motility after thawing of AI doses and during thermodynamic testing. Percentages of live and dead sperm were also evaluated. The data set was analysed using SAS/STAT 9.1 (SAS Institute Inc., Cary, NC, USA). The results confirmed significant (P<0.05-0.01) between-sire differences in the volume, density, and activity of sperm as well as in share of live and dead sperm after collection; in decline of sperm motility in the fresh ejaculate, after thawing, and during the entire thermodynamic test, as well as in the share of live and dead sperm after thawing. The extenders ranked by sperm motility are: Optidyl, Triladyl, AndroMed, and Bioxcell, demonstrating the higher quality of AI doses produced using egg yolk extenders. Differences in sperm motility were significant (P<0.05-0.01) during the entirety of thermodynamic testing. Egg yolk extenders had a significantly (P<0.05-0.01) higher percentages of live sperm after thawing.

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“…The integrity of plasma and acrosomal membranes, as well as mitochondrial function, were evaluated using a combination of propidium iodide (PI), fluorescein isothiocyanate conjugated Pisum sativum agglutinin (FITC-PSA), and 5,5' ,6,6'-tetrachloro-1,1' ,3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1) fluorescent probes, respectively (CELEGHINI et al, 2008). A total of 200 sperm cells were examined per slide and classified according to the fluorescence emitted by each probe: IPIAH = intact plasma membrane, intact acrosomal membrane and high mitochondrial function (represents the "viablecell" percentage in samples); IPIAL = intact plasma membrane, intact acrosomal membrane and low mitochondrial function; IPDAH = intact plasma membrane, damaged acrosomal membrane and high mitochondrial function; IPDAL = intact plasma membrane, damaged acrosomal membrane and low mitochondrial function; DPIAH = damaged plasma membrane, intact acrosomal membrane and high mitochondrial function; DPIAL = damaged plasma membrane, intact acrosomal membrane and low mitochondrial function; DPDAH = damaged plasma membrane, damaged acrosomal membrane and high mitochondrial function; DPDAL = damaged plasma membrane, damaged acrosomal membrane and low mitochondrial function.…”

Section: Methodsmentioning

confidence: 99%

Efeitos da disponibilidade de sombra a campo sobre características reprodutivas de touros da raça Nelore (Bos indicus) criados na região Sudeste do Brasil

Tarragó

Rodrigues

Zaffalon

et al. 2013

Braz. J. Vet. Res. Anim. Sci.

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Solar radiation is responsible for bull body temperature elevation. An alternative to minimize heat stress is to use artificial shade. Thus, this study aimed to evaluate the effect of thermal stress reduction, through shade availability, on reproductive characteristics of Nellore bulls (Bos indicus). For this, ten bulls were divided in: Available artificial shade (AS, n = 5) and Unavailable shade (US, n = 5). Each group was kept in two hectare paddocks, in which shade availability for group AS was artificially created. Animals were submitted to a clinical-reproductive evaluation and seminal analyses. No interaction was observed between treatments (AS and US) and time (8 collections) for all analyzed variables (P>0.05). No significant effect (P > 0.05) of treatment was observed for all parameters analyzed. So, it can be concluded that the absence of shaded areas during summer does not negatively affect reproductive characteristics such as: scrotal circumference, testicular consistency, progressive motility, percentage of rapidly moving cells (Computer Assisted Semen Analysis -CASA), morphology or sperm viability in Nellore bulls raised in southeastern Brazil, considering that results could be different in other regions of the country where average temperature is higher. Keywords:Semen. Artificial shade. Nellore. Bull. ResumoA radiação solar é responsável pelo aumento da temperatura corpórea em touros. Uma alternativa para minimizar o estresse térmico é usar sombreamento artificial. Assim, este estudo teve como objetivo avaliar o efeito da redução do estresse térmico através da disponibilização de sombra artificial, sobre características reprodutivas de touros da raça Nelore (Bos indicus). Para isso, dez touros foram divididos em: Disponibilidade de sombra artificial (AS, n = 5) e Não-disponibilidade de sombra artificial (US, n=5). Cada grupo foi mantido em piquete de dois hectares cada, no qual a sombra para o grupo AS foi criada artificialmente. Os animais foram submetidos a avaliações clínicas-reprodutivas e análise seminal. Nenhuma interação foi observada entre os tratamentos (AS e US) e o tempo (8 coletas), para todas as variáveis analisadas (P > 0.05). Nenhum efeito significativo (P>0.05) de tratamento foi observado para os parâmetros analisados. Dessa forma, concluiu-se que a ausência de áreas sombreadas, durante o verão, não afetou negativamente as características reprodutivas como: circunferência escrotal, consistência testicular, motilidade progressiva, percentagem de células com movimentos rápidos (Computer Assisted Semen Analisys -CASA), morfologia e viabilidade espermática em touros da raça Nelore criados na região sudeste do Brasil, considerando que esse resultado pode ser diferente em outras regiões do país, onde as temperaturas ambientais são mais elevadas.Palavras-chave: Sêmen. Sombreamento artificial. Nelore. Touro.

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Effects that bovine sperm cryopreservation using two different extenders has on sperm membranes and chromatin

Cited by 162 publications

References 25 publications

Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa

Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa

Effect of sire and extender on sperm motility and share of live or dead sperm in bulls’ fresh ejaculate and in AI doses after thawing

Efeitos da disponibilidade de sombra a campo sobre características reprodutivas de touros da raça Nelore (Bos indicus) criados na região Sudeste do Brasil

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