2018
DOI: 10.1016/j.foodchem.2017.06.113
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Abstract: HighlightsWidely differing free asparagine concentration in wheat from two harvest years.Wide range of acrylamide-forming potential (AFP) in 73 commercial wheat varieties.Eight varieties identified with consistently low AFP, seven of them soft types, but benefit of using a low AFP variety is lost if sulphur supply is inadequate.Selecting varieties for low AFP solely on the basis of them being soft would be simplistic.Information on free asparagine concentration should be available at variety launch.

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Cited by 39 publications
(40 citation statements)
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References 24 publications
(40 reference statements)
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“…Chromatograms were analyzed as described by Chope et al 26 Analysis of Free Amino Acids. Free amino acids were extracted according to methods from Curtis et al 27 First, 10 mg samples were suspended and extracted in 400 μL of 0.01 N HCl for 30 min at room temperature. After centrifugation at 10000 rpm for 15 min, the supernatants were filtered through a 0.22 μm poly(ether sulfone) filter before analysis.…”
Section: ■ Materials and Methodsmentioning
confidence: 99%
“…Chromatograms were analyzed as described by Chope et al 26 Analysis of Free Amino Acids. Free amino acids were extracted according to methods from Curtis et al 27 First, 10 mg samples were suspended and extracted in 400 μL of 0.01 N HCl for 30 min at room temperature. After centrifugation at 10000 rpm for 15 min, the supernatants were filtered through a 0.22 μm poly(ether sulfone) filter before analysis.…”
Section: ■ Materials and Methodsmentioning
confidence: 99%
“…Asn is considered to serve as a major transport compound in the xylem from the root to the leaves and in the phloem from the leaves to the developing seeds in several model and leguminous species that have been investigated (Amarante et al , 2006; Lea et al , 2007; Krapp, 2015) This model is also corroborated by the high nitrogen to carbon ratio of Asn (Coruzzi, 2003) Previous studies aiming at determining the AA potential in crop species have revealed that genetic variability exists in the accumulation of Asn in plant organs (Halford et al , 2012; Postles et al , 2013; Curtis & Halford, 2016; Muttucumaru et al , 2017; Curtis et al , 2018) In the present study, we could identify chicory genotypes contrasting for Asn accumulation in storage roots and demonstrate that diversity in Asn accumulation relates to the expression of Asn biosynthetic genes. Noticeably, we found a positive correlation between the gene expression of the Asn biosynthetic genes in leaves and the Asn level in storage roots (figs.…”
Section: Discussionmentioning
confidence: 78%
“…Strategies based on agricultural practices, natural variation and biotechnology have recently been investigated to reduce the AA potential of crop species (Loaëc et al. , 2014; Zhu et al, 2016; Curtis et al, 2018). Asn, besides being a proteinogenic amino acid, plays a central role in nitrogen metabolism in plants, acting in nitrogen transport, recycling and remobilization during development (Gaufichon et al , 2010).…”
Section: Introductionmentioning
confidence: 99%
“…They found two cultivars which showed a reduced level of free Asn if high N was available and S was applied. Curties et al [15] reported that by S supply the level of free Asn was less influenced by year and the cultivar was more stable. In our studies, such effects could not be found.…”
Section: Conventional S Trialmentioning
confidence: 99%