“…3'(Ponton et al, 2011); Dilp8-Fw: 5'-GCA CCA CCA TCT GAA TCG AC-3', Dilp8-Rv: 5'-CTG AGG CGA TTG AAG TGC TC -3'; NLaz-Fw:5'-GCC AGA AGT AGA ACG GAT ACC A-3', NLaz-Rv:5'-ACT GGT GCA GCT GTA GACGAC-3' (Hull-Thompson et al, 2009); Upd1-Fw: 5'-AGA CAG CCG TCA ACC AGA C-3', Upd1-Rv: 5'-AGA CAG CCG TCA ACC AGA C-3' [(Moskalev et al, 2019), wing disc samples)]; Upd1-Fw: 5'-TGT AAC CCC GTT CGC TGT AT-3', Upd1-Rv: 5'-GCT GAT GTT TCC GTT TCC GT-3' (muscle samples); Upd3-Fw: 5'-ATC CCA CCA ATC CCC TGA AG-3', Upd3-Rv: 5'-AGA TTG CAG GTG TTC TCC CA-3'.Quantification and Statistical AnalysisImage Processing and AnalysisFiji [National Institute of Health (NIH) Bethesda, MD] was used to measure the size of the anterior (a), dorsal (d) compartments (based on Ci and MyrT expression respectively), or the whole wing discs (based on DAPI staining). Image stacks were obtained using a Leica TCS SP5 MP confocal microscope, 20X dry objective with 1 µm per optical section to cover the entire thickness of each disc.…”