Effects of ubiquinone on hydroperoxide concentration and antioxidant enzymatic activities in the rat hippocampus during pilocarpine-induced seizures

Santos, Ítala Mônica de Sales; Freitas, Rizângela Lyne Mendes de; Silva, Elisângela Pascoal da; Feitosa, Chistiane Mendes; Saldanha, Gláucio Barros; Souza, Geane Felix de; Tomé, Adriana da Rocha; Feng, Dejiang; Freitas, Rivelilson Mendes de

doi:10.1016/j.brainres.2009.12.037

Cited by 14 publications

(8 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since the neutralisation of the increased ROS formation during seizures depends on the ability of the antioxidant defence systems, the antiepileptogenic therapeutic potential of various substances possessing antioxidant activities, have been extensively studied [ 67 - 69 ]. This includes endogenously present antioxidants, such as α-lipoic acid [ 70 - 73 ], GSH [ 74 ], melatonin [ 75 - 85 ], ubiquinone (coenzyme Q 10 ) [ 86 , 87 ] and vitamin A [ 88 ], exogenous anticonvulsive and neuro-protective substances, such as α-tocopherol [ 53 , 70 - 72 ], ascorbic acid [ 66 , 89 , 90 ], curcumin [ 91 - 100 ], N-acetylcisteine [ 101 ], omega-3 fatty acids [ 102 - 112 ] and resveratrol [ 113 , 114 ], and novel synthetic, potent radical scavengers, like aspalatone [ 115 ], EPC-K1 [ 116 ], EUK-134 [ 117 ], MnTBAP [ 118 - 120 ], and tempol [ 121 ]. These compounds, generally defined as molecules with the ability to quench or reduce highly ROS, exerts neuroprotective effects and can therefore protect the brain against oxidative stress as seen in some experimental models of seizures [ 66 , 70 - 74 , 90 - 92 , 96 , 99 , 122 - 125 ].…”

Section: Antiepileptic Therapy and Oxidative Stressmentioning

confidence: 99%

“…Recently, studies evaluating the neuroprotective effects of ubiquinone in rats’ pilocarpin-induced epileptic model were performed [ 86 , 87 ]. In pilocarpine group, an important increase in hydroperoxide concentration and GPx activity was reported, while there were no changes observed in SOD and CAT activities.…”

Section: Antiepileptic Therapy and Oxidative Stressmentioning

confidence: 99%

“…In pilocarpine group, an important increase in hydroperoxide concentration and GPx activity was reported, while there were no changes observed in SOD and CAT activities. On the other hand, in rat hippocampusof ubiquinone group, markedly decreased hydroperoxide content and elevated SOD, CAT and GPx activities were observed [ 87 ]. Overall, ubiquinone has been reported to decrease the extent of oxidative stress and consequently the severity of pilocarpine-induced seizures [ 86 ].…”

Section: Antiepileptic Therapy and Oxidative Stressmentioning

confidence: 99%

“…These results suggest that ubiquinone may exert significant neuroprotective actions which might be helpful in the treatment of neurodegenerative disorders [ 86 , 87 ].…”

Section: Antiepileptic Therapy and Oxidative Stressmentioning

confidence: 99%

“…Numerous studies in animal models of seizures and epilepsy have been conducted in order to evolve the influence of an add-on antioxidant treatment on enzymatic antioxidant activity (SOD, CAT, GPx and GR), non-enzymatic endogenous antioxidant status (GSH), ROS markers (hydroperoxide), various markers of macromolecular oxidative stress damage (MDA, TBARS, 8-OHdG, mtDNA damage and P-carb), and nitrate/nitrite levels. Practically all antioxidants, including endogenously present α-lipoic acid 70, 73, 143, 204, coenzyme Q 10 [ 87 ] and melatonin 135, 142, 145, 147, 148, exogenous anticonvulsive and neuro- protective substances, such as ascorbic acid [ 66 , 89 , 135 , 153 , 164 , 165 ], curcumin [ 96 , 99 , 100 , 205 , 206 ], ginsenoside-Rd [ 207 - 209 ], propolis [ 175 ], α-tocopherol [ 122 , 125 , 135 , 193 , 201 , 210 ], and naringin [ 211 ], and novel synthetic, potent radical scavengers, like aspalatone [ 115 ], EPC-K1 [ 135 ], and tempol [ 121 ], have shown neuroprotective effects against oxidative stress induced by different proconvulsive substances that are usually used in models of seizures and epilepsy, as summarized in the Table 4 .…”

Section: Studies Of Antioxidants Usage In Various Animal Models Of Epmentioning

confidence: 99%

See 4 more Smart Citations

Antioxidants as a Preventive Treatment for Epileptic Process: A Review of the Current Status

Martinc¹,

Grabnar²,

Vovk

2015

View full text Add to dashboard Cite

Epilepsy is known as one of the most frequent neurological diseases, characterized by an enduring predisposition to generate epileptic seizures. Oxidative stress is believed to directly participate in pathways leading to neurodegeneration, which serves as the most important propagating factor, leading to the epileptic condition and cognitive decline. Moreover, there is also a growing body of evidence showing the disturbance of antioxidant system balance and consequently increased production of reactive species in patients with epilepsy. A meta-analysis, conducted in the present review confirms an association between epilepsy and increased lipid peroxidation. Furthermore, it was also shown that some of the antiepileptic drugs could potentially be responsible for additionally increased lipid peroxidation. Therefore, it is reasonable to propose that during the epileptic process neuroprotective treatment with antioxidants could lead to less sever structural damages, reduced epileptogenesis and milder cognitive deterioration. To evaluate this hypothesis studies investigating the neuroprotective therapeutic potential of various antioxidants in cells, animal seizure models and patients with epilepsy have been reviewed. Numerous beneficial effects of antioxidants on oxidative stress markers and in some cases also neuroprotective effects were observed in animal seizure models. However, despite these encouraging results, till now only a few antioxidants have been further applied to patients with epilepsy as an add-on therapy. Based on the several positive findings in animal models, a strong need for more carefully planned, randomized, double-blind, cross-over, placebo-controlled clinical trials for the evaluation of antioxidants efficacy in patients with epilepsy is warranted.

show abstract

Section: Antiepileptic Therapy and Oxidative Stressmentioning

confidence: 99%

Section: Antiepileptic Therapy and Oxidative Stressmentioning

confidence: 99%

Section: Antiepileptic Therapy and Oxidative Stressmentioning

confidence: 99%

“…These results suggest that ubiquinone may exert significant neuroprotective actions which might be helpful in the treatment of neurodegenerative disorders [ 86 , 87 ].…”

Section: Antiepileptic Therapy and Oxidative Stressmentioning

confidence: 99%

Section: Studies Of Antioxidants Usage In Various Animal Models Of Epmentioning

confidence: 99%

See 3 more Smart Citations

Antioxidants as a Preventive Treatment for Epileptic Process: A Review of the Current Status

Martinc¹,

Grabnar²,

Vovk

2015

View full text Add to dashboard Cite

show abstract

Ellagic acid and allopurinol decrease H2O2 concentrations, epileptiform activity and astrogliosis after status epilepticus in the hippocampus of adult rats

et al. 2022

View full text Add to dashboard Cite

Neuroprotective Effects of Idebenone Against Pilocarpine-Induced Seizures: Modulation of Antioxidant Status, DNA Damage and Na+, K+-ATPase Activity in Rat Hippocampus

Ahmed

2014

Neurochem Res

View full text Add to dashboard Cite

The current study investigated the neuroprotective activity of idebenone against pilocarpine-induced seizures and hippocampal injury in rats. Idebenone is a ubiquinone analog with antioxidant, and ATP replenishment effects. It is well tolerated and has low toxicity. Previous studies reported the protective effects of idebenone against neurodegenerative diseases such as Friedreich's ataxia and Alzheimer's disease. So far, the efficacy of idebenone in experimental models of seizures has not been tested. To achieve this aim, rats were randomly distributed into six groups. Two groups were treated with either normal saline (0.9 %, i.p., control group) or idebenone (200 mg/kg, i.p., Ideb200 group) for three successive days. Rats of the other four groups (P400, Ideb50 + P400, Ideb100 + P400, and Ideb200 + P400) received either saline or idebenone (50, 100, 200 mg/kg, i.p.) for 3 days, respectively followed by a single dose of pilocarpine (400 mg/kg, i.p.). All rats were observed for 6 h post pilocarpine injection. Latency to the first seizure, and percentages of seizures and survival were recorded. Surviving animals were sacrificed, and the hippocampal tissues were separated and used for the measurement of lipid peroxides, total nitrate/nitrite, glutathione and DNA fragmentation levels, in addition to catalase and Na(+), K(+)-ATPase activities. Results revealed that in a dose-dependent manner, idebenone (100, 200 mg/kg) prolonged the latency to the first seizure, elevated the percentage of survival and diminished the percentage of pilocapine-induced seizures in rats. Significant increases in lipid peroxides, total nitrate/nitrite, DNA fragmentation levels and catalase activity, in addition to a significant reduction in glutathione level and Na(+), K(+)-ATPase activity were observed in pilocarpine group. Pre-administration of idebenone (100, 200 mg/kg, i.p.) to pilocarpine-treated rats, significantly reduced lipid peroxides, total nitrate/nitrite, DNA fragmentation levels, and normalized catalase activity. Moreover, idebenone prevented pilocarpine-induced detrimental effects on brain hippocampal glutathione level, and Na(+), K(+)-ATPase enzyme activity in rats. Data obtained from the current investigation emphasized the critical role of oxidative stress in induction of seizures by pilocarpine and elucidated the prominent neuroprotective and antioxidant activities of idebenone in this model.

show abstract

Effects of ubiquinone on hydroperoxide concentration and antioxidant enzymatic activities in the rat hippocampus during pilocarpine-induced seizures

Cited by 14 publications

References 30 publications

Antioxidants as a Preventive Treatment for Epileptic Process: A Review of the Current Status

Antioxidants as a Preventive Treatment for Epileptic Process: A Review of the Current Status

Ellagic acid and allopurinol decrease H2O2 concentrations, epileptiform activity and astrogliosis after status epilepticus in the hippocampus of adult rats

Neuroprotective Effects of Idebenone Against Pilocarpine-Induced Seizures: Modulation of Antioxidant Status, DNA Damage and Na+, K+-ATPase Activity in Rat Hippocampus

Contact Info

Product

Resources

About