Effects of type II collagen hydrolysates on osteoarthritis through the NF-κB, Wnt/β-catenin and MAPK pathways

Li, Hao; Li, Zhaoxia; Wang, Yanchao; Zhao, Xue; Yu, Mingxiao; Hou, Hu

doi:10.1039/d1fo03414f

Cited by 10 publications

(4 citation statements)

References 50 publications

(67 reference statements)

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“…Western blot analysis was referred to as the method of Hao et al 23 Protein lysate and protease inhibitors were used to extract the kidney protein, which was then separated by using sulfate polyacrylamide gel electrophoresis. The protein was then transferred to poly(vinylidene fluoride) membranes, which were subsequently blocked with skimmed milk and incubated for 12 h with the primary antibodies against β-actin, glucose transporter type 9 (GLUT9), organic anion transporter 1 (OAT1), ATP-binding cassette subfamily G2 (ABCG2), tumor necrosis factor receptor-associated factor (TRAF6), NF-κB p65, and TLR4 (1:500).…”

Section: Western Blot Analysismentioning

confidence: 99%

Identification of Hyperuricemia Alleviating Peptides from Yellow Tuna Thunnus albacares

Hao,

Ding,

Fan

et al. 2024

J. Agric. Food Chem.

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The development of food-derived antihyperuricemic substances is important for alleviating hyperuricemia (HUA) and associated inflammation. Here, novel peptides fromThunnus albacares (TAP) with strong antihyperuricemic activity were prepared. TAP was prepared by alkaline protease (molecular weight <1000 Da), with an IC 50 value of xanthine oxidase inhibitory activity of 2.498 mg/mL, and 5 mg/mL TAP could reduce uric acid (UA) by 33.62% in human kidney-2 (HK-2) cells (P < 0.01). Mice were fed a high-purine diet and injected with potassium oxonate to induce HUA. Oral administration of TAP (600 mg/kg/d) reduced serum UA significantly by 42.22% and increased urine UA by 79.02% (P < 0.01) via regulating urate transporters GLUT9, organic anion transporter 1, and ATP-binding cassette subfamily G2. Meantime, TAP exhibited hepatoprotective and nephroprotective effects, according to histological analysis. Besides, HUA mice treated with TAP showed anti-inflammatory activity by decreasing the levels of toll-like receptor 4, nuclear factors-κB p65, NLRP3, ASC, and Caspase-1 in the kidneys (P < 0.01). According to serum non-targeted metabolomics, 91 differential metabolites between the MC and TAP groups were identified, and purine metabolism was considered to be the main pathway for TAP alleviating HUA. In a word, TAP exhibited strong antihyperuricemic activity both in vitro and in vivo.

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Section: Western Blot Analysismentioning

confidence: 99%

Identification of Hyperuricemia Alleviating Peptides from Yellow Tuna Thunnus albacares

Hao,

Ding,

Fan

et al. 2024

J. Agric. Food Chem.

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“… Rats Ma et al [ 134 ] Cartilage hydrolysates Bovine Oral, 200 & 500 mg/kg/day Cartilage degeneration is reduced. Rats Hao et al [ 135 ] Collagen peptides <1.5 kDa Fish cartilage and skin In vitro , 50–100 μg/mL, 3 days The synthesis of collagen types I and II was enhanced. Equine articular chondrocyte organoids Bourdon et al [ 136 ] Collagen peptide Iridescent shark catfish ( Pangasius hypophthalmus ) skin Oral, 100–200 mg/kg/day, 12 weeks Cartilage damage and loss of proteoglycan reduced.…”

Section: Fdps In the Management Of Ramentioning

confidence: 99%

Food-derived bioactive peptides potentiating therapeutic intervention in rheumatoid arthritis

Liu,

Yan,

Zhang

et al. 2024

Heliyon

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“…The medial meniscectomy (MMx) method was used for modeling. The modeling methods are as follows: with reference to the previous research methods of the research group, the rat OA model was established by the MMx method (Hao et al, 2022). The medical nursing mat sheet was laid on the animal experimental table.…”

Section: Grouping and Modeling Of Animal Experimentsmentioning

confidence: 99%

Gastrodin Ameliorates Knee Joint Inflammation and Pain in Osteoarthritis Rats and Prevents Chondrocyte Injury by Regulating PI3K/Akt/FoxO1 Pathway

Shi,

Liu,

Dong

et al. 2023

Pharmacognosy Magazine

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Background Several studies have demonstrated the anti-inflammatory properties of gastrodin. However, its specific role and mechanism in the context of osteoarthritis (OA) remain unclear. Objectives The objective of this study was to explore the function and mechanism of gastrodin in both in vivo and in vitro OA models. Materials and Methods In this study, the targets of gastrodin against osteoarthritis were analyzed using bioinformatics methods. The effect of gastrodin on neutrophil infiltration and inflammatory cytokines in OA synovial tissue was evaluated using H&E staining. The levels of inflammatory cytokines were determined using ELISA and Q-PCR. The extracellular matrix degradation-related proteins and PI3K/AKT/FoxO1 signaling pathway were tested using western blotting. Results Bioinformatics analysis predicted that the target of gastrodin against OA might act on the FoxO pathway. The use of gastrodin resulted in a significant reduction in the infiltration of neutrophils in the synovial tissue of rats, as observed through H&E staining. Additionally, gastrodin was found to attenuate IL-1β-induced inhibition of chondrocyte viability, pro-inflammatory cytokine production, and extracellular matrix degradation. This effect can be attributed to the suppression of PI3K/AKT/FoxO1 by gastrodin. Conclusion These findings indicate that gastrodin is a treatment for OA.

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Effects of type II collagen hydrolysates on osteoarthritis through the NF-κB, Wnt/β-catenin and MAPK pathways

Abstract: Osteoarthritis (OA), a degenerative disease, has attracted extensive attention all over the world. In the study, medial meniscus resection (MMx) and anterior to medial collateral ligament (ACL) operation rat model...

Cited by 10 publications

References 50 publications

Identification of Hyperuricemia Alleviating Peptides from Yellow Tuna Thunnus albacares

Identification of Hyperuricemia Alleviating Peptides from Yellow Tuna Thunnus albacares

Food-derived bioactive peptides potentiating therapeutic intervention in rheumatoid arthritis

Gastrodin Ameliorates Knee Joint Inflammation and Pain in Osteoarthritis Rats and Prevents Chondrocyte Injury by Regulating PI3K/Akt/FoxO1 Pathway

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