Effects of trapidil on ATPase, lipid peroxidation, and correlation with ultrastructure in experimental spinal cord injury

Göçer, A. İskender; İldan, Faruk; Tuna, Metin; Polat, Sait; Tamer, L.; Erman, Tahsin; Kaya, Mehmet

doi:10.1007/pl00012398

Cited by 21 publications

(4 citation statements)

References 29 publications

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“…Previous studies have shown that trapidil significantly reduces cellular damage and edema in the injury site. This finding is explained by the membrane stabilizing effect of trapidil by inhibition of TXA 2 synthesis [16]. It was reported that trapidil had a marked protection against IR injury in testis.…”

Section: Discussionmentioning

confidence: 92%

Ischemia-reperfusion injury of rabbit ovary and protective effect of trapidil: an experimental study

et al. 2007

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We aimed to detect the protective effect of trapidil in ischemia-reperfusion (IR) injury due to ovarian torsion and detorsion. Thirty-two pubertal New Zealand albino rabbits were used. Adnexal torsion was created by rotating the left adnexa including the tubal and ovarian vessels in a 360 degrees clockwise direction. Adnexal detorsion was done by untwisting the adnexa. In the IR group, left oopherectomy was performed after 3 h of adnexal torsion and 3 h of adnexal detorsion. In the study group, a 3-h adnexal torsion was performed and trapidil was administered intraperitoneally as a single dose of 40 mg/kg, 1 h before detorsion. The left oopherectomy was performed after a 3-h adnexal detorsion. In the sham group, sham operation was performed followed by left oopherectomy. In the control group, normal ovarian tissue was evaluated. Catalase, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) levels of ovarian tissue were determined for each group. The values of SOD and GSH-Px activities in the IR group were significantly decreased (P < 0.05). In addition, the MDA level was significantly higher in the IR group (P < 0.01). The trapidil-administered group showed significant increase in the levels of GSH-Px (P < 0.05), catalase (P < 0.05), SOD (P < 0.05), and decreased MDA levels (P < 0.05) compared to those in the IR group. The study has shown that trapidil treatment prevents ischemia induced oxidative damage in the ovarian tissues of rabbits.

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Section: Discussionmentioning

confidence: 92%

Ischemia-reperfusion injury of rabbit ovary and protective effect of trapidil: an experimental study

et al. 2007

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“…This finding is explained by the membrane stabilizing effect of trapidil by inhibiting of TXA 2 synthesis. 34 It was reported that trapidil has a marked protection against IR injury in peripheral nerves. 35 It was reported that trapidil has positive effects on anastomotic healing due to its preventive effects on inflammatory response and lipid peroxidation.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of trapidil in ischemia–reperfusion injury due to testicular torsion and detorsion: An experimental study

Somuncu¹,

Çakmak²,

Erdoğan³

et al. 2006

Int J of Urology

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Objective: We aimed to detect the preventive effects of trapidil in ischemia-reperfusion (IR) injury due to testicular torsion and detorsion. Methods: Forty prepubertal albino rats were used. In the IR group, torsion was created by rotating the left testis over 2 h, and detorsion was done by untwisting the testis. Bilateral orchiectomies were performed after 4 h. In study group, 2-h torsion was performed and trapidil was administered as a single dose 1 h before detorsion. Bilateral orchiectomies were performed after 4 h. In the sham group, a sham operation was done. In the sham plus trapidil group, a sham operation was done and trapidil was administered as a single dose. Testicular tissue malondialdehyde (MDA), nitric oxide (NO) and total sulfhydryl (T-SH) levels were determined for each group. The grades of interstitial injury were determined in histopathologic examination. Results: The NO and MDA levels in the IR group were significantly higher than the study, sham and sham plus trapidil groups in the left testis (P < 0.05, P < 0.001 and P < 0.001, respectively). A statistical difference was not found among study, sham and sham plus trapidil groups in the left testis in NO and MDA levels (P > 0.05). The T-SH level in the study group was significantly higher than in the IR, sham and sham plus trapidil groups in left testis P < 0.05). In the IR group (left testis), grade 1 interstitial injury was 30% (3/10), grade 2 injury was 60% (6/10) and grade 3 injury was 10% (1/10). In the study group (left testis), grade 1 interstitial injury was 30% (3/10) and there was no injury in 70% (7/10). Conclusion: Trapidil decreased free oxygen radical formation in testicular torsion and detorsion, and attenuated histopathological damage in the ipsilateral twisted testis.

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“…Increased lipid peroxidationcombined with decreased tissue Na + -K + ATPase activity may be associated with an impairment in the interaction of Na + -K + ATPase with membrane phospholipids [27, 28]. In addition, the contribution of oxidative modifications of thiol groups of the enzyme itself may be of importance, as well as the direct inhibitory effect of ethanol reported in vitro [29].…”

Section: Discussionmentioning

confidence: 99%

Alcohol-Induced Lung Damage and Increased Oxidative Stress

Aytaçoğlu

Çalıkoğlu²,

Tamer³

et al. 2006

Respiration

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Background: Alcohol-induced lung damage may be associated with increased oxidative stress. Objective: Our aim was to investigate alcohol-induced changes in the biochemistry and histopathology of the lung. Methods: Rats were divided into two groups, a control group and an ethanol group. The ethanol group received 2 g/kg ethanol (total: 3 ml) intraperitoneally. The controls were given the same amount of saline via the same route. Three hours later, the rats were sacrificed, and blood and lung tissue samples were obtained. Oxidative stress was assessed by measuring the levels of erythrocyte reduced glutathione (GSH), tissue malondialdehyde (MDA), myeloperoxidase (MPO) and Na⁺-K⁺ ATPase. Histopathologic evaluation of the lung tissues was also performed. Results: In the ethanol group, serum and tissue MDA levels and MPO activities were increased (p = 0.007, p = 0.001 and p = 0.000), and lung tissue Na⁺-K⁺ ATPase activities and erythrocyte GSH were decreased (p = 0.001 and p = 0.000) compared to the controls. Histopathologic examination demonstrated alveolocapillary thickening, alveolar degeneration, leukocyte infiltration and erythrocyte extravasation in the lungs of the ethanol group (p < 0.05). Conclusion: These results suggest that high-doseacute alcohol administration aggravates systemic and local oxidative stress leading to acute lung injury, ranging from mild pulmonary dysfunction to severe lung injury. It should be borne in mind that rapid onset of the acute respiratory distress syndrome (ARDS) may also be due to increased oxidative stress following alcohol abuse, especially when ischemic disturbances, e.g. coronary heart disease, acute ischemia of the extremities and traumatic accidents, are concomitantly present. Therefore, precautions against ARDS may prevent morbidity and mortality in alcohol-induced lung damage in at-risk patients.

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Effects of trapidil on ATPase, lipid peroxidation, and correlation with ultrastructure in experimental spinal cord injury

Cited by 21 publications

References 29 publications

Ischemia-reperfusion injury of rabbit ovary and protective effect of trapidil: an experimental study

Ischemia-reperfusion injury of rabbit ovary and protective effect of trapidil: an experimental study

Protective effects of trapidil in ischemia–reperfusion injury due to testicular torsion and detorsion: An experimental study

Alcohol-Induced Lung Damage and Increased Oxidative Stress

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