Effects of Thermotherapy on Th1/Th2 Cells in Esophageal Cancer Patients Treated with Radiotherapy

Hong, Min Ki; Zao, Jiang; Yingfeng, Zhou

doi:10.7314/apjcp.2014.15.5.2359

Cited by 30 publications

(24 citation statements)

References 21 publications

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“…However, when dysfunction occurs, the balance is disturbed, known as 'the shifting of Th1/Th2 balance' (18). In addition, this may cause different types of diseases, such as cancer (19)(20)(21), AIDS (22) and autoimmune diseases (23). When the balance drifts from Th1 to Th2, it may cause antitumor immunosuppression (24).…”

Section: Introductionmentioning

confidence: 99%

Effect of xanthohumol on Th1/Th2 balance in a breast cancer mouse model

et al. 2017

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Xanthohumol (XN), a prenylflavonoid found in the hop plant, Humulus lupulus, exhibits a variety of biological activities. Numerous studies have reported that XN inhibits the growth of many types of cancer cells, but the effects of XN on tumor immunity have not yet been studied. We explored the effect of XN on Th1/Th2 balance and the underlying mechanism based on a BALB/c-4T1 breast cancer mouse model. The results showed that XN significantly slowed down tumor growth and inhibited expression of antitumor proliferation protein Ki-67 as well as breast cancer-specific marker cancer antigen 15-3 (CA15-3). Flow cytometric analysis revealed that XN enhanced the secretion of perforin, granzyme B and increased the ratio of CD8+/CD25+. ELISA analysis of cytokine results demonstrated that XN obviously upregulated Th1 cytokines, while downregulated Th2 cytokines. Th1/Th2 ratio analysis by flow cytometry illustrated that XN regulated the balance drift to Th1 polarization. Western blotting and immunohistochemistry (IHC) results manifested that XN induced expression of T-bet, a Th1-specific transcription factor. Furthermore, we found that XN significantly promoted the phosphorylation of signal transducer and activator of transcription (STAT)4. Our results demonstrated that XN promoted Th1/Th2 balance towards Th1 polarization, and STAT4 may play a positive role in the regulation of Th1/Th2 cytokines by XN.

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Section: Introductionmentioning

confidence: 99%

Effect of xanthohumol on Th1/Th2 balance in a breast cancer mouse model

et al. 2017

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“…Interferon gamma (IFNγ) is one of the central cytokines that coordinates tumor immune responses and the associated biological consequences [ 2 ]. IFN-γ is a pleiotropic cytokine with multiple biological functions including immune cell activation [ 3 , 4 ] and induction of the major histocompatibility complex (MHC) molecules both in normal and neoplastic cells [ 5 , 6 ]. Besides its ability to trigger cell cycle arrest and apoptosis [ 7 – 10 ], IFN-γ shows anti-tumor activity in patients with advanced head and neck squamous cell carcinoma (HNSCC) [ 11 ] or non-small-cell lung carcinoma (NSCLC) [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Interferon gamma-induced apoptosis of head and neck squamous cell carcinoma is connected to indoleamine-2,3-dioxygenase via mitochondrial and ER stress-associated pathways

Jamal¹,

Taylor²,

Elmageed³

et al. 2016

Cell Div

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BackgroundTumor response to immunotherapy is the consequence of a concerted crosstalk between cytokines and effector cells. Interferon gamma (IFNγ) is one of the common cytokines coordinating tumor immune response and the associated biological consequences. Although the role of IFNγ in the modulation of tumor immunity has been widely documented, the mechanisms regulating IFNγ-induced cell death, during the course of immune therapy, is not described in detail.ResultsIFNγ triggered apoptosis of CLS-354 and RPMI 2650 cells, enhanced the protein expression and activation of indoleamine 2,3-dioxygenase (IDO), and suppressed the basal expression of heme oxygenase-1(HO-1). Interestingly, IFNγ induced the loss of mitochondrial membrane potential (Δψm) and increased accumulation of reactive oxygen species (ROS). The cytokine also induced the activation of Janus kinase (JAK)/Signal Transducer and Activator of Transcription (STAT)1, apoptosis signal-regulating kinase 1 (ASK1), p38, c-jun-N-terminal kinase (JNK) and NF-κB pathways and the transcription factors STAT1, interferon regulatory factor 1 (IRF1), AP-1, ATF-2, NF-κB and p53, and expression of Noxa protein. Furthermore, IFNγ was found to trigger endoplasmic reticulum (ER) stress as evidenced by the cleavage of caspase-4 and activation of protein kinase RNA-like endoplasmic reticulum kinase (PERK) and inositol-requiring-1α (IRE1α) pathways. Using specific inhibitors, we identified a potential role for IDO as apoptotic mediator in the regulation of IFNγ-induced apoptosis of head and neck squamous cell carcinoma (HNSCC) cells via Noxa-mediated mitochondrial dysregulation and ER stress.ConclusionIn addition to the elucidation of the role of IDO in the modulation of apoptosis, our study provides new insights into the molecular mechanisms of IFNγ-induced apoptosis of HNSCC cells during the course of immune therapy.

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“…In a clinical study, the 5-year survival rate of cervical cancer patients with high CD4 + / CD8 + ratio was higher than that of patients with low CD4 + / CD8 + ratio, increasing the CD4 + / CD8 + ratio can slow the progression of cervical cancer and improve its prognosis (21) . It is generally believed that Th1 enhances tumor immune surveillance of tumor while Th2 associated with the tumor immune evasion can suppress the function of Th1 cells (22) . Th17 can promote tumor angiogenesis in the promotion of tumor progression (6) .…”

Section: Discussionmentioning

confidence: 99%

Sufentanil Postoperative Analgesia reduce the increase of T helper 17 (Th17) cells and FoxP 3 + regulatory T (Treg) cells in Rat Hepatocellular Carcinoma Surgical Model: A randomised animal study

Peng

Guo

Zheng

et al. 2020

Preprint

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Background: Several investigations have indicated that T helper cell 17 (Th 17 ) and Foxp 3 + regulatory T cells (Treg) cells adopt a pro-tumorigenic role. Opioids and pain exacerbate immunosuppression in immunocompromised cancer patients. Methods: A rat hepatocellular carcinoma (HCC) models was established by N-nitrosodiethylamine (DENA). 48 of them were randomly divided into 3 groups (n=16): surgery without postoperative analgesia (Control); surgery with morphine postoperative analgesia (Morphine); surgery with sufentanil postoperative analgesia (Sufentanil). The level of cluster of CD4 + , CD8 + , Th1,Th2, Th17 and Treg cells in blood were also detected to assess immune function using flow cytometry on d0, d3 and d7.The rats’ survival situation of each group left after three days of surgery were observed. Results ： The CD4 + /CD8 + ratio and Th1 cells levels were significantly higher while Th2, Th17 and Treg cells levels were significantly lower in sufentanil and morphine postoperative analgesia rats compared with that without postoperative analgesia. Compared with morphine postoperative analgesia, sufentanil postoperative analgesia rats seem have higher CD4+/CD8+ ratio , Th1 cells, while lower Th2, Th17 and Treg cells level. Conclusions ： Sufentanil and morphine postoperative analgesia show better immune function than that without postoperative analgesia by analyzing CD4 + /CD8 + ratio, Th1, Th2, Th17 and Treg cells. Sufentanil postoperative analgesia demonstrated a favorable impact on immune function compared with morphine postoperative anesthesia in hepatocellular carcinoma rats undergoing left hepatectomy operation.

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Effects of Thermotherapy on Th1/Th2 Cells in Esophageal Cancer Patients Treated with Radiotherapy

Cited by 30 publications

References 21 publications

Effect of xanthohumol on Th1/Th2 balance in a breast cancer mouse model

Effect of xanthohumol on Th1/Th2 balance in a breast cancer mouse model

Interferon gamma-induced apoptosis of head and neck squamous cell carcinoma is connected to indoleamine-2,3-dioxygenase via mitochondrial and ER stress-associated pathways

Sufentanil Postoperative Analgesia reduce the increase of T helper 17 (Th17) cells and FoxP 3 + regulatory T (Treg) cells in Rat Hepatocellular Carcinoma Surgical Model: A randomised animal study

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