Effects of thermal hydrolytic pre-treatment on biogas process efficiency and microbial community structure in industrial- and laboratory-scale digesters

Westerholm, Maria; Castillo, María del Pilar; Andersson, Ann-Christin; Nilsen, Paal Jahre; Schnürer, Anna

doi:10.1016/j.wasman.2019.06.004

Cited by 37 publications

(13 citation statements)

References 55 publications

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“…Despite that the reference reactors operated under constant parameters, and similar to the full-scale reactors from which the inoculums were taken, quite drastic dynamics were observed in the microbial community under the first phase of operation (Figure 2; Supplementary Figure S3). These changes were thus likely related to altered conditions associated with downscaling the processes, including changed feeding interval, which previously has been shown to influence performance and bacterial structure (Mulat et al, 2015;Westerholm et al, 2019). Transition to lab-scale conditions promoted the family Rikenellaceae (Bacteroidetes) in A ref (fed with cattle and poultry manure), where its relative abundance increased from 5%-6% to 42%-64% (phases 1-2 of A ref , Supplementary Figure S2).…”

Section: Initial Microbial Community Profile and The Effect Of Down-scalingmentioning

confidence: 91%

Anaerobic Digestion of Animal Manure and Influence of Organic Loading Rate and Temperature on Process Performance, Microbiology, and Methane Emission From Digestates

Ahlberg-Eliasson¹,

Westerholm²,

Isaksson³

et al. 2021

Front. Energy Res.

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Biogas production from manure is of particular value in regard of lowering greenhouse gas emissions and enhancing nutrient re-circulation. However, the relatively low energy content and the characteristics of manure often result in low degradation efficiency, and the development of operating strategies is required to improve the biogas yield and the economic benefits. In this study, the potential to enhance the performance of two full-scale biogas plants operating with cattle manure, in mono-digestion or combined with poultry manure, was investigated. Four continuously fed laboratory-scale reactors were operated in sets of two, in which the temperature in one reactor in each set was increased from 37–42°C to 52°C. The potential to increase the capacity was thereafter assessed by increasing the organic loading rate (OLR), from ca 3 to 5 kg volatile solids (VS)/ m3 and day. The processes were evaluated with both chemical and microbiological parameters, and in addition, the residual methane potential (RMP) was measured to evaluate the risk of increased methane emissions from the digestate. The results showed that both processes could be changed from mesophilic to themophilic temperature without major problems and with a similar shift in the microbial community profile to a typical thermophilic community, e.g., an increase in the relative abundance of the phylum Firmicutes. However, the temperature increase in the reactor co-digesting cattle and poultry manure caused a slight accumulation of fatty acids (2 g/l) and reduced the specific methane production, most likely due to ammonia inhibition (0.4–0.7 g NH3/l). Still, during operation at higher OLR, thermophilic as compared to mesophilic temperature slightly increased the methane yield and specific methane production, in both investigated processes. However, the higher OLR decreased the overall degree of degradation in all processes, and this showed a positive correlation with increased RMP values. Chemical analyses suggested that high RMP values (40–98 Nml gVS−1) were related to the degradation of cellulose, hemicellulose, and volatile fatty acid enriched in the digestate. Conclusively, increased temperature and load can increase the methane yield from manure but can result in less efficient degradation and increased risks for methane emissions during storage and handling of the digestate.

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Section: Initial Microbial Community Profile and The Effect Of Down-scalingmentioning

confidence: 91%

Anaerobic Digestion of Animal Manure and Influence of Organic Loading Rate and Temperature on Process Performance, Microbiology, and Methane Emission From Digestates

Ahlberg-Eliasson¹,

Westerholm²,

Isaksson³

et al. 2021

Front. Energy Res.

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“…To improve the understanding of highly diverse and interconnected networks of AD microbiomes, several studies focused on the taxonomic and functional characterization of microbial communities originating from laboratory-scale biogas reactors [8][9][10][11][12][13][14][15][16][17] as well as from full-scale biogas plants [18][19][20][21][22][23] trying to connect microbiome compositions to prevailing process parameters [4,24]. Other studies focused on the identification of the functional roles of particular species isolated from AD systems [25][26][27].…”

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confidence: 99%

New insights from the biogas microbiome by comprehensive genome-resolved metagenomics of nearly 1600 species originating from multiple anaerobic digesters

Campanaro

Treu

Rodriguez-R

et al. 2020

Biotechnol Biofuels

169

123

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Background: Microorganisms in biogas reactors are essential for degradation of organic matter and methane production. However, a comprehensive genome-centric comparison, including relevant metadata for each sample, is still needed to identify the globally distributed biogas community members and serve as a reliable repository. Results: Here, 134 publicly available metagenomes derived from different biogas reactors were used to recover 1635 metagenome-assembled genomes (MAGs) representing different biogas bacterial and archaeal species. All genomes were estimated to be > 50% complete and nearly half ≥ 90% complete with ≤ 5% contamination. In most samples, specialized microbial communities were established, while only a few taxa were widespread among the different reactor systems. Metabolic reconstruction of the MAGs enabled the prediction of functional traits related to biomass degradation and methane production from waste biomass. An extensive evaluation of the replication index provided an estimation of the growth dynamics for microbes involved in different steps of the food chain. Conclusions: The outcome of this study highlights a high flexibility of the biogas microbiome, allowing it to modify its composition and to adapt to the environmental conditions, including temperatures and a wide range of substrates. Our findings enhance our mechanistic understanding of the AD microbiome and substantially extend the existing repository of genomes. The established database represents a relevant resource for future studies related to this engineered ecosystem.

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“…Efficient anaerobic digestion depends on the biological activity of electron/acetic acid-producing and electron-consuming/methanogens of the digestion process in question. , After 58 days of the experiment, high-resolution 16S rRNA pyrophosphate sequencing of anaerobic sludge in the four reactors was performed, leading to microbial diversity analysis of bacteria and archaea in each reactor. Figure A shows the genus-level bacterial community structure of anaerobic digestion of propionate/butyrate.…”

Section: Resultsmentioning

confidence: 99%

Nickel Foam Promotes Syntrophic Metabolism of Propionate and Butyrate in Anaerobic Digestion

Zhou

et al. 2021

ACS Omega

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Enhanced interspecies electron transfer (IET) among symbiotic microorganisms is an effective method to increase the rate of methane (CH 4 ) production in anaerobic digestion. Direct interspecies electron transfer (DIET), which does not involve dissolved redox media, is considered an alternative and superior method to enhance methane production by interspecific hydrogen (H 2 ) transfer (IHT). In this study, nickel foam was built into a semicontinuous anaerobic reactor to investigate its effect on the metabolism of propionate and butyrate. Both increased the average yield of CH 4 in anaerobic digestion by 18.1 and 15.9%, respectively. Analysis of bacterial and archaeal communities showed that the addition of nickel foam could increase the relative abundance of microbial communities involved in DIET and could increase the diversity of microorganisms in the reactor. Moreover, the anaerobic digestion performance of the nickel foam reactor was good at high hydrogen partial pressure.

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Effects of thermal hydrolytic pre-treatment on biogas process efficiency and microbial community structure in industrial- and laboratory-scale digesters

Cited by 37 publications

References 55 publications

Anaerobic Digestion of Animal Manure and Influence of Organic Loading Rate and Temperature on Process Performance, Microbiology, and Methane Emission From Digestates

Anaerobic Digestion of Animal Manure and Influence of Organic Loading Rate and Temperature on Process Performance, Microbiology, and Methane Emission From Digestates

New insights from the biogas microbiome by comprehensive genome-resolved metagenomics of nearly 1600 species originating from multiple anaerobic digesters

Nickel Foam Promotes Syntrophic Metabolism of Propionate and Butyrate in Anaerobic Digestion

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