Effects of Thai black sticky rice extract on oxidative stress and lipid metabolism gene expression in HepG2 cells

Sangkitikomol, W; Tencomnao, Tewin; Rocejanasaroj, A

doi:10.4238/vol9-4gmr912

Cited by 35 publications

(16 citation statements)

References 22 publications

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“…The 200 and 400 mg/L TLE doses progressively increased CYP3A4 and PPARg gene expression, respectively. This particular biological activity of TLE was similar to that of black sticky rice extract, which acted in a concentration-dependent fashion with a biphasic "up then down" effect on LDLR mRNA expression in HepG2 cells (Sangkitikomol et al, 2010). PPARg plays essential roles in metabolic syndromes.…”

Section: Discussionmentioning

confidence: 67%

Thunbergia laurifolia extract minimizes the adverse effects of toxicants by regulating P-glycoprotein activity, CYP450, and lipid metabolism gene expression in HepG2 cells

2014

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ABSTRACT. Thunbergia laurifolia (TL) is widely used as an antidote in Thai traditional medicine against toxic substances such as alcohol, pesticides, arsenic, and strychnine. We found that the lyophilized form of TL in 80% ethanol possessed the antioxidant levels within the range 23,163.9 ± 1457.4 Trolox equivalents mM/kg dry mass and 899.8 ± 14.5 gallic acid equivalents mM/kg dry mass using the oxygen radical absorbance capacity assay and the Folin Ciocalteu phenol assay, respectively. TL extract (TLE) at a high dose (3000 mg/L) induced cytotoxicity according to the neutral red assay and the MTT assay. However, TLE doses of 800-3000 mg/L could reduce intracellular oxidative stress in a dose-dependent manner (P < 0.05) using the dichlorodihydrofluorescein diacetate assay. TLE significantly enhanced the mRNA expression of CYP1A1, CYP1A2, CYP2B6, CYP3A4, and PPARg, but it significantly inhibited the mRNA expression of CYP3A7, CYP2D6, and CYP2E1 (P < 0.05) by reverse transcription-polymerase chain reaction. Moreover, TLE could increase the activity of a multidrug transporter, P-glycoprotein, which accelerated the excretion of toxic substances from HepG2 cells. It is suggested that TLE may be beneficial for detoxification by reducing oxidative stress, minimizing toxicity by regulating the expression CYP450 mRNAs for suitable production of CYP450 isoenzymes, and increasing PPARg mRNA expression and P-glycoprotein activity in HepG2 cells, thereby maintaining xenobiotic biotransformation balance.

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Section: Discussionmentioning

confidence: 67%

Thunbergia laurifolia extract minimizes the adverse effects of toxicants by regulating P-glycoprotein activity, CYP450, and lipid metabolism gene expression in HepG2 cells

2014

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“…Other natural products have been used to examine lipid lowering effects, such as hesperidin, naringin (Jung et al, 2006), resveratrol (Do et al, 2008), and curcumin (Jang et al, 2008), which could also decrease hepatic HMG-CoAR. In contrast, red wine polyphenols (Pal et al, 2003) and anthocyanins in black sticky rice (Sangkitikomol et al, 2010) have been shown to increase hepatic LDLR. In addition, β-sitosterol in M. oleifera leaves has also been shown to decrease intestinal uptake of cholesterol (Lin et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Effect of Moringa oleifera on advanced glycation end-product formation and lipid metabolism gene expression in HepG2 cells

Sangkitikomol¹,

Rocejanasaroj²,

Tencomnao³

2014

Genet. Mol. Res.

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ABSTRACT. In Thai traditional medicine, Moringa oleifera is used for the treatment of diabetes and hyperlipidemia. Oxidative stress plays a major role in the pathogenesis of many degenerative diseases, such as hyperlipidemia, diabetes mellitus, and cardiovascular disease. We evaluated the antioxidant effect of M. oleifera extract (MOE) for reduction of advanced glycation end-product (AGE) formation, cell viability, oxidative stress, and lipid metabolism gene expression in HepG2 cells. We found that the lyophilized form of MOE in 80% ethanol possessed mean (± SD) total antioxidant, polyphenolic, and flavonoid contents of 9307 ± 364 TE mM/kg dry mass, 218 ± 1 GE mM/kg dry mass, and 286 ±12 QE mM/kg dry mass, determined using an oxygen radical absorbance capacity assay, a Folin Ciocalteu phenol assay, and a total flavonoids assay, respectively. Concentrations of 2.5-10.0 mg/ mL MOE could inhibit AGE-formation by 10-45%, and 100-1000 mg/L MOE reduced intracellular oxidative stress (P < 0.05) in a dose-dependent manner in the DCFH-DA assay. However, MOE induced cytotoxicity at high doses (2000-3000 mg/L), as shown by the MTT assay. MOE significantly downregulated the mRNA expression of the HMG-CoAR, PPARα1, and PPARγ genes (P < 0.05). We concluded that M. oleifera could have benefits for human health by reducing oxidative stress and AGE formation. Moreover, M. oleifera may reduce cholesterol and lipid synthesis by suppression of HMG-CoAR, PPARα1, and PPARγ gene expression, thereby maintaining lipid homeostasis.

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“…47 Also, oxidative stress-induced ROS production was observed in HepG2 cells treated with an anthocyaninrich extract from Thai black sticky rice. 48 According to literature data the in vitro antioxidant potential of anthocyanins is related to their highly electrondeficient form of flavylium cation. 44 Crucial for cell-target interaction seems to be the ortho-3 0 ,4 0 -dihydroxy groups on the B ring.…”

Section: Intracellular Rosmentioning

confidence: 99%

Antioxidant Activities of Chokeberry Extracts and the Cytotoxic Action of Their Anthocyanin Fraction on HeLa Human Cervical Tumor Cells

Rugină

Sconţa²,

Leopold³

et al. 2012

Journal of Medicinal Food

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The present study evaluates the antioxidant activity of two Aronia melanocarpa cultivars-Viking and Aronand of Aronia prunifolia hybrid in relationship with their phytochemical composition regarding the contents of total phenolics, flavonoids, procyanidins, and monomeric anthocyanins. The antioxidant capacity of the mentioned extracts of chokeberries was evaluated through five complementary assays: 2,2 0 -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), H2O2 scavenging potential, oxygen radical absorbance capacity, ferric reducing antioxidant power, and cupric ion reducing antioxidant capacity. A. prunifolia hybrid was found to have the highest antioxidant activity and to be the richest in polyphenols, procyanidins, and anthocyanins compared with the A. melanocarpa cultivars. A good correlation was observed between antioxidant activity and total procyanidin and anthocyanin content. Cyanidin glycosides inhibited HeLa human cervical tumor cell proliferation and increased generation of reactive oxygen species after 48 h of treatment, suggesting that they could be responsible for the antiproliferative activity. These results may be significant for industry concerning food quality and disease prevention.

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Effects of Thai black sticky rice extract on oxidative stress and lipid metabolism gene expression in HepG2 cells

Cited by 35 publications

References 22 publications

Thunbergia laurifolia extract minimizes the adverse effects of toxicants by regulating P-glycoprotein activity, CYP450, and lipid metabolism gene expression in HepG2 cells

Thunbergia laurifolia extract minimizes the adverse effects of toxicants by regulating P-glycoprotein activity, CYP450, and lipid metabolism gene expression in HepG2 cells

Effect of Moringa oleifera on advanced glycation end-product formation and lipid metabolism gene expression in HepG2 cells

Antioxidant Activities of Chokeberry Extracts and the Cytotoxic Action of Their Anthocyanin Fraction on HeLa Human Cervical Tumor Cells

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