Effects of stress produced by adrenocorticotropin (ACTH) on lipid peroxidation and some antioxidants in vitamin C treated and nontreated chickens

Abstract

The aim of this study was to examine the effects of vitamin C on malonaldehyde (MDA) and glutathione (GSH) concentrations, and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity in chickens stressed by adrenocorticotropin (ACTH). Sixty Leghorn chickens (20 weeks old) were randomly allotted to a control and a vitamin C treated group. An isotonic sodium chloride solution was administrated intramuscularly for a period of five days to the control group at a dose of 2.5 mL per chicken per day, and the treatment group received a vitamin C solution (containing 250 mg vitamin C/2.5 mL) intramuscularly for five days. On the fifth day of the experiment the chickens in both groups received 50 IU ACTH intramuscularly. Three hours after ACTH application blood samples were collected to determine the concentrations of MDA and GSH, and activities of SOD and GSH-Px in the blood. The concentration of MDA in the control group increased compared to that in the vitamin C treated group. After the ACTH application the activity of SOD increased in both groups, thought significantly so in the vitamin C treated group. The GSH-Px activity did not differ significantly between the treatments after ACTH application. It was concluded that intramuscularly administrated vitamin C facilitated an adaptation against stress and decreased negative effects of stress in chickens.

The ameliorative effect of ascorbic acid on the oxidative status, live weight and recovery rate in road transport stressed goats in a hot humid tropical environment

“…Belge et al . () also reported that AA exerts its antioxidative effect by removing singlet oxygen, hydroperoxyl, superoxide, lipid peroxyl and lipid free radicals, and thus decreases MDA concentration in animals subjected to stress. The significant increase in MDA activity recorded in groups B and C are in agreement with that of Nazifi et al .…”

“…the administration of AA even after transport reduced the effect of stress and enhance regaining of lost weight. Lipid peroxidation is the oxidative deterioration of polyunsaturated lipids, which leads to the production of a degraded product, called MDA (Belge et al 2003). The increase in MDA activity in group A following transportation was not significant as compared to groups B and C which recorded significant MDA activity following transportation.…”

“…AA is a free radical scavenger, which protects the body defense system against excessively produced free radicals during stress and stabilizes the health status of animals (Sivakumar et al 2010). Belge et al (2003) also reported that AA exerts its antioxidative effect by removing singlet oxygen, hydroperoxyl, superoxide, lipid peroxyl and lipid free radicals, and thus decreases MDA concentration in animals subjected to stress. The significant increase in MDA activity recorded in groups B and C are in agreement with that of Nazifi et al (2009) and Kataria et al (2010) who reported increased MDA activities following transportation in cattle and camels, respectively.…”

Effects of Vitamins C and E on Erythrocyte Osmotic Fragility, Serum Malondialdehyde concentrations and Surface Erythrocyte Sialic Acid in Rams following Road Transportation

“…Avellini et al (1995) noted that ROS are known to play a vital role in tissue damage and has adverse effects on erythrocyte. It is known fact ROS are generated in animals subjected to stress (Halliwell, 1996) leading to lipid peroxidation a complex process which leads to membrane damage and cell death (Belge et al, 2003). Lipid peroxidation is the oxidative deterioration of polyunsaturated lipids, leading to the production of a degraded product called malondialdehyde (Belge et al, 2003) in which its concentration is a biomarker for ROS generation.…”

“…It is known fact ROS are generated in animals subjected to stress (Halliwell, 1996) leading to lipid peroxidation a complex process which leads to membrane damage and cell death (Belge et al, 2003). Lipid peroxidation is the oxidative deterioration of polyunsaturated lipids, leading to the production of a degraded product called malondialdehyde (Belge et al, 2003) in which its concentration is a biomarker for ROS generation. The result of the present study showed that MDA concentration as well as the degree of haemolysis increases as the journey progresses especially in group 3 (control) which was not administered any antioxidant.…”