2013
DOI: 10.1007/s10616-013-9635-6 View full text |Buy / Rent full text
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Abstract: The present investigation was carried out to evaluate the effect of stress hormone cortisol on the myogenic markers in the C2C12 cells co-cultured with 3T3-L1 preadipocytes. Co-culturing was achieved by transwell inserts with a 0.4 lm porous membrane. C2C12 and 3T3-L1 cells were grown independently on the transwell plates. After differentiation, inserts containing 3T3-L1 cells were transferred to C2C12 plates for co-culturing. 10 lg/ll of cortisol was added to the medium. After 72 h of treatment, C2C12 cells w… Show more

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“…Herein, we found the same tendency of the high expression of ID2 , ID3 and CXCR4 in the individuals with Del-Del genotype that was accompanied by PAX7 level. However, the expression of other four genes, MYF5 , MYOD , MRF4 and MYOG , were not associated with the genotypes of the PAX7 10-bp indel, which may be explained by the reason that the four genes play crucial roles in proliferation and differentiation of myoblast cells, especially, the MRF4 and MYOG are expressed in a later stage of myogenesis 44 . Therefore, the PAX7 10-bp indel could be utilized in early selection project for cattle breeding.…”
Section: Discussionmentioning
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“…Herein, we found the same tendency of the high expression of ID2 , ID3 and CXCR4 in the individuals with Del-Del genotype that was accompanied by PAX7 level. However, the expression of other four genes, MYF5 , MYOD , MRF4 and MYOG , were not associated with the genotypes of the PAX7 10-bp indel, which may be explained by the reason that the four genes play crucial roles in proliferation and differentiation of myoblast cells, especially, the MRF4 and MYOG are expressed in a later stage of myogenesis 44 . Therefore, the PAX7 10-bp indel could be utilized in early selection project for cattle breeding.…”
Section: Discussionmentioning
“…In the myogenic differentiation process numerous signaling pathways promote the formation of skeletal muscle [ 6 ]. The basic helix-loop-helix proteins and the mammalian target of rapamycin (mTOR) signaling pathway are promotors of skeletal myogenesis and the multiple stages of myogenic differentiation through distinct mechanisms [ 7 8 ]. Le Grand and Rudnicki [ 9 ] indicated that the expression of myogenin, myogenic regulatory factors 4 (MRF4), myosin heavy chain I (MyHC I), and myogenic differentiation antigen are activated and controlled during differentiation.…”
Section: Introductionmentioning