Effects of soluble copper and copper oxide nanoparticle exposure on the immune system of mussels, <scp><i>Mytilus galloprovincialis</i></scp>

Torres‐Duarte, Cristina; Hutton, S. P.; Vines, Carol A.; Moore, James G.; Cherr, Gary N.

doi:10.1002/tox.22684

Cited by 11 publications

(5 citation statements)

References 44 publications

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“…In hemocyte cultures exposed to CuCl, the percentages of M. galloprovincialis and R. philippinarum cells that engulfed the target yeast cells significantly decreased beginning from a concentration of 0.5 μM (49.5 µg L −1 ) ( p < 0.001) ( Figure 2B ). Similar results have been reported after bivalve hemocyte exposure to Cu(II) ions beginning from concentrations of 50 μg L -1 for M. galloprovincialis ( Torres-Duarte et al, 2019 ) and 60 μg L -1 for R. philippinarum ( Matozzo et al, 2001 ). These authors interpreted these observations by analyzing the relationship between the changes in phagocytic activity and alterations in cytoskeleton organization.…”

Section: Resultssupporting

confidence: 87%

“… ↑, increased and ↓, decreased effect relative to controls. 1) Matozzo et al, 2001 ; 2) Gómez-Mendikute and Cajaraville, 2003 ; 3) Torres-Duarte et al, 2019 ; 4) Katsumiti et al, 2018 ; 5) Viarengo et al, 1994 ; 6) the present work. …”

Section: Resultssupporting

confidence: 51%

“…Consequently, changes in the activities of antioxidant molecules and enzymes are useful indexes in the evaluation of the toxicity of an environmental contaminant. In the hemolymph of M. galloprovincialis , extensive oxidative stress was indicated by various immunohistochemical parameters after exposure to metals like cadmium ( Koutsogiannaki et al, 2014 ) and copper ( Torres-Duarte et al, 2019 ) and a significant increase in the production of ROS, followed by oxidative damage identified by DNA fragmentation, protein carbonylation and lipid peroxidation, has been reported.…”

Section: Resultsmentioning

confidence: 99%

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Immunotoxic effects of exposure to the antifouling copper(I) biocide on target and nontarget bivalve species: a comparative in vitro study between Mytilus galloprovincialis and Ruditapes philippinarum

Cima,

Varello

2023

Front. Physiol.

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Edible bivalves constitute an important bioresource from an economic point of view, and studies on their immune responses to environmental pollutants are crucial for both the preservation of biodiversity and economic reasons. The worldwide diffusion of copper(I)-based antifouling paints has increased copper leaching into coastal environments and its potential impact on both target and nontarget organisms. In this study, immunotoxicity assays were carried out with short-term (60 min) cultures of hemocytes from the bivalves Mytilus galloprovincialis—a mussel dominant in the macrofouling community—and Ruditapes philippinarum—a clam dominant in the soft-sediment community—exposed to CuCl to compare the toxic effects on their immune responses. The LC50 values were similar, 40 μM (3.94 mg L-1) for the mussel and 44 μM (4.33 mg L-1) for the clam. In both species, apoptosis occurred after exposure to 1 µM (98.9 μg L-1) CuCl, the concentration able to significantly increase the intracellular Ca2+ content. Biomarkers of cell morphology and motility revealed microfilament disruption, a significant decrease in yeast phagocytosis and lysosome hydrolase (β-glucuronidase) inhibition beginning from 0.5 µM (49.5 μg L-1) CuCl in both the mussel and clam. The same concentration of CuCl affected biomarkers of oxidative stress, as a significant decrease in reduced glutathione content in the cytoplasm and inhibition of mitochondrial cytochrome-c oxidase (COX) were detected in both species. Comparison of the biomarkers showed that clam is more sensitive than the mussel regarding alterations to the lysosomal membrane and reactive oxygen species (ROS) production, which supports the potential harmful effects of antifouling biocides on the survival of nontarget pivotal species in the coastal community.

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Section: Resultssupporting

confidence: 87%

Section: Resultssupporting

confidence: 51%

Section: Resultsmentioning

confidence: 99%

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Immunotoxic effects of exposure to the antifouling copper(I) biocide on target and nontarget bivalve species: a comparative in vitro study between Mytilus galloprovincialis and Ruditapes philippinarum

Cima,

Varello

2023

Front. Physiol.

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“…Increased phagocytosis and reactive oxygen species production were induced only by nCuO which suggest a "particle effect" and inflammation. In marine mussels exposed to nCuO and Cu 2+ , the latter caused significant Cu accumulation in gills and the hemolymph (Torres-Duarte et al, 2019). Although nCuO did not cause accumulation of Cu in tissues, damage to gill tissues and ingestion by hemocyte were observed.…”

Section: Discussionmentioning

confidence: 90%

Comparative Toxicity of Copper Oxide Nanoparticles and Dissolved Copper to Freshwater Mussels

Auclair¹,

Turcotte²,

Gagnon³

et al. 2019

IJZI

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The commercial use of copper oxide nanoparticles (nCuO) has raised concerns about the toxic risk to filter feeders such as bivalves. The purpose of this study was to compare the toxicity of nCuO and dissolved Cu 2+ to the freshwater mussel Dreissena bugensis. Mussels were exposed to a range of concentrations of both forms of Cu (2, 10 and 50 µg/L) for 96 h at 15 C. After the exposure period, some mussels were kept aside to determine the air survival time as a measure of resistance to stress. The remaining mussels were processed for total and labile Cu determination in their tissues and the following effects: lipid peroxidation (LPO), DNA damage, arachidonate cyclooxygenase (COX), protein-ubiquitin levels (UB), acetylcholinesterase (AChE) and glutathione S-transferase (GST) activity. The data revealed that only exposure to Cu 2+ led to the accumulation of total Cu in tissues with a decrease in labile Cu 2+ levels suggesting that Cu was strongly bound to tissues. Exposure to nCuO led to specific effects on COX activity (inflammation) and UB levels (damaged protein turnover). It is concluded that although no significant changes in Cu levels in mussels were detected, exposure to nCuO produced different effects than Cu 2+ in freshwater mussels.

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“…On the other hand, the conclusions were done for oral application and it cannot be generalized for other routes of particles application. In a paper by Torres-Duarte and coworkers, there was a described effect of copper oxide nanoparticles on Mediterranean mussels (Mytilus galloprovincialis), when the mussels were contemporary infected with pathogenic bacteria (164). The authors exposed the mussels to concentration of copper nanoparticles in a range from 100 to 450 μg/l and let them expose to pathogenic bacterium Vibrio tubiashii.…”

Section: Copper Nanoparticlesmentioning

confidence: 99%

Copper and copper nanoparticles toxicity and their impact on basic functions in the body

Pohanka¹

2019

BLL

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Copper is a biogenic metal having multiple functions in basic processes in organisms and it is common in all kingdoms of life. Limited intake of copper is a problem; however, doses of copper exceeding the recommended alimentary source are problematic as well and toxicity is soon manifested. Impact of copper nanoparticles on human health is another serious issue taken into consideration in this review. Regarding to copper toxicity, neurodegenerative disorders including Alzheimer and Parkinson diseases are suspected to be linked to copper toxicity or copper can contribute to their progression. Wilson and Menke diseases are also described as examples of copper intolerance. This paper is focused on the description, literature survey and discussion of the current knowledge about copper and copper nanoparticles toxicity and their involvement in various pathological processes (Tab. 6, Fig. 2, Ref. 171).

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Effects of soluble copper and copper oxide nanoparticle exposure on the immune system of mussels, Mytilus galloprovincialis

Abstract: Copper and copper oxide nanomaterials (nCuO) can enter the marine environment negatively impacting mussels, an environmental and commercially relevant organism. We analyzed the effects on the immune system of adult mussels exposed to soluble copper (CuSO 4 , 20-50 μg/L)

Cited by 11 publications

References 44 publications

Immunotoxic effects of exposure to the antifouling copper(I) biocide on target and nontarget bivalve species: a comparative in vitro study between Mytilus galloprovincialis and Ruditapes philippinarum

Immunotoxic effects of exposure to the antifouling copper(I) biocide on target and nontarget bivalve species: a comparative in vitro study between Mytilus galloprovincialis and Ruditapes philippinarum

Comparative Toxicity of Copper Oxide Nanoparticles and Dissolved Copper to Freshwater Mussels

Copper and copper nanoparticles toxicity and their impact on basic functions in the body

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