Effects of small interfering RNA targeting heparanase-1 combined with heparin on invasiveness of mouse hepatocellular carcinoma cell lines

Yu, Shengjin; Kang, Xiaohui; Zhang, Jianing; Wang, Hongmei; Xie, Tao; Wang, Wei; Wang, Shujing

doi:10.5732/cjc.009.10746

Cited by 7 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the following experiment, we demonstrated that pmiR-HPSE-1 can lead to the obvious decrease in the invasion and metastasis capabilities of HCC cells. Therefore, downregulation of HPSE expression could result in suppression of invasion and metastasis abilities of HCC cells, which were similar to other studies [ 13 – 15 ].…”

Section: Discussionsupporting

confidence: 90%

“…Heparanase (HPSE) is an endo-beta-glucuronidase that is capable of cleaving heparan sulfate (HS) side chains of heparan sulfate proteoglycans (HSPGs) on cell surfaces and extracellular matrices (ECM) of basement membrane (BM) and plays critical roles in tumor cell invasion, migration, and angiogenesis by remodeling ECM and delivering some cytokines such as basic fibroblast growth factor (bFGF) and vascular endothelial cell growth factor (VEGF) [ 8 – 10 ]. Increased HPSE expression was found in numerous tumor types and correlates with poor prognosis [ 11 , 12 ], and downregulation of heparanase expression results in suppression of tumor invasion and migration, especially in HCC cells [ 13 – 15 ]. In recent 15 years, HPSE has become a research hotspot [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Downregulation of Heparanase Expression Results in Suppression of Invasion, Migration, and Adhesion Abilities of Hepatocellular Carcinoma Cells

Chen

Luo

Tian

et al. 2015

BioMed Research International

View full text Add to dashboard Cite

Objective. Heparanase (HPSE) is high-expressed in most malignant tumors including hepatocellular carcinoma (HCC) and promotes cancer cell invasion and migration. The aim of the study is to explore whether HPSE enhances adhesion in metastasis of HCC cells. Methods. HPSE expressions in human HCC cells were measured with real-time RT-PCR and Western blot analysis. Four recombinant miRNA vectors pcDNATM6.2-GW/EmGFP-miR-HPSE (pmiR-HPSE) were transfected into HCCLM3 cell. HPSE expression in transfected cell was measured. The cell invasion, migration, and adhesion abilities were detected, respectively. Results. Both HPSE mRNA and protein relative expression levels were higher in HepG2, BEL-7402, and HCCLM3 cells than those in normal hepatocyte (P < 0.05). HPSE showed highest expression level in HCCLM3 cell (P < 0.05). Transfection efficiencies of four miRNA vectors were 75%–85%. The recombinant vectors significantly decreased HPSE expression in transfected HCCLM3 cells (P < 0.01), and pmiR-HPSE-1 showed best interference effect (P < 0.05). pmiR-HPSE-1 significantly decreased the penetrated and migrating cells numbers and adherence rate of HCCLM3 cells (P < 0.05). Conclusion. HPSE is a potentiator of cell adhesion in metastasis of HCC.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Downregulation of Heparanase Expression Results in Suppression of Invasion, Migration, and Adhesion Abilities of Hepatocellular Carcinoma Cells

Chen

Luo

Tian

et al. 2015

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…It has been proved that RNAi-induced heparanase silencing can inhibit cell invasion of gastric carcinoma [19], hepatocellular carcinoma [20] and embryonal rhabdomyosarcoma [21]. Traditional gene-targeting therapy for cancer is at the post-transcriptional level, silencing the corresponding mRNA.…”

Section: Discussionmentioning

confidence: 99%

Gene silencing of heparanase results in suppression of invasion and migration of hepatoma cells

et al. 2014

View full text Add to dashboard Cite

BackgroundThis study investigated the effect of transcriptional gene silencing (TGS) of the heparanase gene on hepatoma SMCC-7721 cells.MethodsSiRNAs targeting the promoter region and coding region of the heparanase gene were designed and synthesized. Then the siRNAs were transfected into hepatoma SMCC-7721 cells by nuclear transfection or cytoplasmic transfection. The expression of heparanase was detected by RT-PCR and Western blotting 48 h, 72 h and 96 h post-transfection. In addition, wound healing and invasion assays were performed to estimate the effect of TGS of the heparanase gene on the migration and invasion of hepatoma SMCC-7721 cells.ResultsProtein and mRNA expression of the heparanase gene were interfered with by TGS or post-transcriptional gene silencing (PTGS) 48 h after transfection. At 72 h post-transfection, the expression of the PTGS group of genes had recovered unlike the TGS group. At 96 h post-transfection, the expression of the heparanase gene had recovered in both the TGS group and PTGS group. Invasion and wound healing assays showed that both TGS and PTGS of the heparanase gene could inhibit invasion and migration of hepatoma SMCC-7721 cells, especially the TGS group.ConclusionsTGS can effectively interfere with the heparanase gene to reduce the invasion and migration of hepatoma SMCC-7721 cells.

show abstract

“…Cell culture. Hca-F cell counting, passage, culture, recovery and cryopreservation were performed as previously described (13,14). Cells expanded as a single-layer suspension when observed under an inverted phase-contrast microscope (Olympus Co., Tokyo, Japan).…”

Section: In Vitro Experimentsmentioning

confidence: 99%

Treatment of liver cancer in mice by the intratumoral injection of an octreotide-based temperature-sensitive gel

Zhang

Duan

et al. 2013

International Journal of Molecular Medicine

View full text Add to dashboard Cite

Octreotide (OCT) can inhibit tumor growth with few side‑effects. In this study, we hypothesized that an OCT- and poloxamer 407 (P407)-based temperature‑sensitive gel may compensate for the short half‑life of OCT, which may thus lead to the development of a novel therapy for patients with end‑stage liver cancer by intratumoral injection. The proliferation and apoptosis of mouse Hca‑F hepatocellular carcinoma cells were determined by MTT assay and Annexin V‑PI staining. A mouse model of hepatocellular carcinoma was established by the subcutaneous transplantion of Hca‑F cells and OCT‑P407 or OCT solution were injected into the tumors, followed by the detection of OCT levels by high performance liquid chromatography (HPLC) over a specific time period. OCT‑P407, ethanol, OCT, P407 or normal saline (NS) were injected into the tumors and the tumor size, weight and inhibition rate were measured 8 days later. Additionally, the expression of somatostatin receptor‑2 (SSTR‑2), vascular endothelial growth factor (VEGF) and caspase‑3 was detected by immunohistochemistry and RT‑PCR. Compared with the OCT group, the tumor inhibition rate and the apoptotic rate in the OCT‑P407 group were higher and the effects were longer. The tumor size and weight in the OCT‑P407 group were lower and the tumor inhibition rate higher compared with the OCT, P407 and NS groups, with the exception of the ethanol group. The protein and mRNA expression of SSTR‑2 and caspase‑3 in the OCT‑P407 group was higher, and that of VEFG was lower compared with the other groups, with the exception of the ethanol group. In the present study, we demonstrate that the intratumoral injection of OCT‑P407 maintains OCT local effective concentration and prolongs its action time, with a greater therapeutic effect than that of OCT on its own. Although ethanol is more effective in certain aspects, its tumor inhibitory effects are similar to OCT‑P407 and as such, OCT‑P407 may be a suitable alternative.

show abstract

Effects of small interfering RNA targeting heparanase-1 combined with heparin on invasiveness of mouse hepatocellular carcinoma cell lines

Cited by 7 publications

References 0 publications

Downregulation of Heparanase Expression Results in Suppression of Invasion, Migration, and Adhesion Abilities of Hepatocellular Carcinoma Cells

Downregulation of Heparanase Expression Results in Suppression of Invasion, Migration, and Adhesion Abilities of Hepatocellular Carcinoma Cells

Gene silencing of heparanase results in suppression of invasion and migration of hepatoma cells

Treatment of liver cancer in mice by the intratumoral injection of an octreotide-based temperature-sensitive gel

Contact Info

Product

Resources

About