Effects of salt stress on growth, antioxidant enzyme and phenylalanine ammonia-lyase activities in Jatropha curcas L. seedlings

Abstract

The effects of increasing NaCl concentrations on biomass, superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and phenylalanine ammonia-lyase (PAL) in Jatropha curcas L. seedlings were investigated. The fresh weights of cotyledons and radicles with increasing NaCl concentrations decreased progressively, and the fresh weight of hypocotyls reached the lowest level at NaCl concentration of 150 mmol and then increased. SOD activity in the cotyledons, hypocotyls and radicles increased gradually up to NaCl concentrations of 150, 200 and 150 mmol, respectively. The highest POD activities in the cotyledons, hypocotyls and radicles were observed at NaCl concentrations of 150, 200 and 150 mmol, respectively. CAT activity in the cotyledons, hypocotyls and radicles enhanced gradually up to 100, 200 and 150 mmol NaCl concentrations, respectively. Increased PAL activity in the hypocotyls and radicles was linearly and positively correlated with increasing NaCl concentrations, but the peak activity in the cotyledons was observed at NaCl concentration of 150 mmol. Electrophoresis analysis suggested that different patterns in SOD and POD isoenzymes depend on NaCl concentrations and organ type, and the staining intensities of these isoforms are consistent with the changes of enzyme activity assayed in solutions.

Changes in antioxidant enzymes activities and proline, total phenol and anthocyanine contents inHyssopus officinalisL. plants under salt stress

“…Increasing concentrations of salt stress caused a significant increase in total CAT activity in the leaves and roots of hyssop plants. Similar to our findings, increased CAT activity in Cassia angustifolia L. [1], maize [4], Jatropha curcas [17], Calendula officinalis L. [12] and Sesamum indicum [24] differing in salt tolerance was found. The results show that increased CAT activity coordinated with the changes of SOD and POD activities plays an important protective role in the ROS-scavenging process and the active involvement of these enzymes are related, at least in part, to salt-induced oxidative stress tolerance in Hyssopus officinalis plants.…”

“…GPX activity increased with salinity concentration, a significant increase in GPX activity was observed in leaves and roots. These results are consistent with the findings of [17]. In leaves of rice plant, salt stress preferentially enhances the content of H 2 O 2 and the activities of SOD, APX, and GPX, whereas it decreases catalase activity [35].…”

Incompatibilidade de enxertia em Prunus

“…Este comportamento tem sido amplamente relacionado a diferentes formas de estresses em tecidos vegetais e resulta em dano oxidativo secundário, devido ao acúmulo de espécies reativas de oxigênio (ROS), como o peróxido de hidrogênio (H 2 O 2 ) (CARNEIRO et al, 2012;WANG et al, 2012). As ROS têm potencial de interagir com muitos componentes celulares e a sua alta concentração gera estresse oxidativo, podendo levar à morte celular, que, quando ocorre, se caracteriza por necrose dos tecidos (GAO et al, 2008;MULLINEAUX & BAKER, 2010;IAKIMOVA et al, 2013), sintoma comum na região de união de combinações incompatíveis.…”

Canola Seedling Response to NaCl Stress – a Proteomic Approach

“…CAT and POX constitute a more efficient system to scavenge H2O2, which may contribute to salt tolerance (Lin and Kao, 2000). Increased activity of antioxidant enzymes upon salt stress, their involvement in salt tolerance in plants (Gao et al, 2008). However, influenced by some mechanisms, unknown to us, amount and activity of these enzymes remain unchanged.…”