Effects of Safflower Seed Oil in Osteoporosis Induced-Ovariectomized Rats

Abstract

The effects of Korean Safflower (Carthamus tinctorius L) seed oil (SSO) on osteoporosis induced-ovariectomized rats were investigated. A total of 90 female Sprague-Dawley rats, 4-month-old, weighing 200-230 g were randomly assigned into 3 groups (30 animals/group). The animals in group I were sham operated and those of group II and III were ovariectomized (Ovx). After eight weeks, the animals of group I and II received solvent vehicle daily, whereas those of group III were administered SSO orally (1 ml/kg) daily for 30 days. The changes in the serum levels of insulin-like growth factor-I (IGF-I), IGF-II, insulin-like growth factor binding protein-3 (IGBP-3), estrogen, total alkaline phosphatase (TALP), bone-specific alkaline phosphatase (BALP), calcium and phosphorous in serum, and also the histomorphology of the proximal tibia metaphysis and femur/body weight (F/B) ratio were examined in all the groups at every 10 days interval. Thirty days later, IGF-I, IGF-II, IGBP-3 and BALP levels were significantly increased (p < 0.05) in group III as compared to groups I and II. There were no significant differences in serum levels of estrogen, TALP and F/B ratio between groups II and III, but estrogen levels were higher in group I. These results suggest that the safflower seeds have possible roles in the improvement of osteoporosis induced-ovariectomized rats.

Changes of Serum Concentrations of Alkaline Phosphatase and Metalloproteinase-9 in an Ovariectomized Wistar Rat Model of Osteoporosis

“…Our study has shown that the levels of Ca 2+ , P and Mg 2+ are not changed, which is typical of the initial phase of osteoporosis, during which no signi cant loss of bone mineral density due to active bone remodeling and a compensatory increased osteoblast activity is seen [22,23]. Weight gain in animals in the G2 proves that lack of estrogens due to OVX leads to obesity [24,25].…”

Effect of safflower seeds supplementation on stimulation of the proliferation, differentiation and mineralization of osteoblastic MC3T3-E1 cells

“…Recently, several results suggested that the safflower seeds have possible roles in the improvement of osteoporosis induced-ovariectomized rats. The safflower seeds have a protecting effect on bone loss caused by estrogen deficiency (Alam et al, 2006;Hong et al, 2002). Therefore, the beneficial effect of safflower seeds has been suggested to be mediated, at least Fig.…”

Effects of the Dichloromethane Fraction of Dipsaci Radix on the Osteoblastic Differentiation of Human Alveolar Bone Marrow-Derived Mesenchymal Stem Cells

“…24,25) Safflower seeds are commonly used as a natural remedy to treat bone fractures in traditional Korean medicine, and have recently been used in regenerative applications. 26,27) Other data on safflower seeds include application to the treatment of periodontal 1-wall intrabony defects, 28) and in the prevention of bone resorption caused by estrogen deficiency. 8) Angelica sinensis has effects on osteoblast proliferation and differentiation.…”