Effects of rhamnolipids on cell surface hydrophobicity of PAH degrading bacteria and the biodegradation of phenanthrene

Zhao, Zhenyong; Selvam, Ammaiyappan; Wong, Jonathan W C

doi:10.1016/j.biortech.2010.11.088

Cited by 123 publications

(51 citation statements)

References 39 publications

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“…This phenomenon is influenced by CSH of the microorganisms. Changes in CSH during petroleum hydrocarbon degradation in the presence of surfactants have been reported by various researchers (Zhao et al 2011;Owsianiak et al 2009;Hua et al 2003;Zhang and Miller 1994). Zhang and Miller (1994) observed that the biosurfactant rhamnolipid increased the CSH of the slow degraders and this enhanced the rate of degradation of octadecane by two strains of P. aeruginosa.…”

Section: Discussionmentioning

confidence: 88%

“…Owsianiak et al (2009) reported that rhamnolipid and Triton X-100 modified the CSH (as determined by BATH assay) of a microbial consortia during biodegradation of diesel fuel, but this increase was not correlated with increase in hydrocarbon degradation. Zhao et al (2011) illustrated different effects of rhamnolipid addition on phenanthrene degradation by a hydrophobic Bacillus subtilis strain and a hydrophilic P. aeruginosa strain. The former depicted reduction in hydrophobicity (as determined by nitrocellulose filter test) and increased lag in biodegradation, while the latter depicted increased hydrophobicity and enhanced biodegradation.…”

Section: Discussionmentioning

confidence: 99%

“…Bioavailability refers to accessibility of substrate to the microorganisms. Bioavailability can be increased by increasing the concentration of hydrocarbons in the aqueous phase by mass transfer from the non-aqueous phase liquid (NAPL) and also by favoring attachment of the microorganisms to the NAPL phase for direct uptake (Mohanty and Mukherji 2008a;Zhao et al 2011). While the transfer of hydrocarbons to the aqueous phase would tend to enhance the rate of degradation by any microorganism, attachment of microorganisms to the hydrocarbons would enhance degradation only if the culture is inherently capable of direct interfacial uptake of hydrocarbons and when they possess the necessary enzymes.…”

Section: Introductionmentioning

confidence: 99%

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Alteration in cell surface properties of Burkholderia spp. during surfactant-aided biodegradation of petroleum hydrocarbons

Mohanty

Mukherji

2011

Appl Microbiol Biotechnol

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Chemical surfactants may impact microbial cell surface properties, i.e., cell surface hydrophobicity (CSH) and cell surface charge, and may thus affect the uptake of components from non-aqueous phase liquids (NAPLs). This work explored the impact of Triton X-100, Igepal CA 630, and Tween 80 (at twice the critical micelle concentration, CMC) on the cell surface characteristics of Burkholderia cultures, Burkholderia cepacia (ES1, aliphatic degrader) and Burkholderia multivorans (NG1, aromatic degrader), when grown on a six-component model NAPL. In the presence of Triton X-100, NAPL biodegradation was enhanced from 21% to 60% in B. cepacia and from 18% to 53% in B. multivorans. CSH based on water contact angle (50-52°) was in the same range for both strains while zeta potential at neutral pH was -38 and -31 mV for B. cepacia and B. multivorans, respectively. In the presence of Triton X-100, their CSH increased to greater than 75° and the zeta potential decreased. This induced a change in the mode of uptake and initiated aliphatic hydrocarbon degradation by B. multivorans and increased the rate of aliphatic hydrocarbon degradation in B. cepacia. Igepal CA 630 and Tween 80 also altered the cell surface properties. For B. cepacia grown in the presence of Triton X-100 at two and five times its CMC, CSH increased significantly in the log growth phase. Growth in the presence of the chemical surfactants also affected the abundance of chemical functional groups on the cell surface. Cell surface changes had maximum impact on NAPL degradation in the presence of emulsifying surfactants, Triton X-100 and Igepal CA630.

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Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Alteration in cell surface properties of Burkholderia spp. during surfactant-aided biodegradation of petroleum hydrocarbons

Mohanty

Mukherji

2011

Appl Microbiol Biotechnol

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“…Further more, their growth on aliphatic hydrocarbons is usually accompanied by the synthesis of biosurfactants. Suggested mechanisms for the uptake of hydrophobic contaminants by degrad ing bacteria include direct contact of the substrates with microorganisms having a high cell surface hydropho bicity and biosurfactantmediated uptake by micro organisms capable of producing biosurfactants (Zhao et al, 2011). Thus, when grown on phenol and hexa decane strain BN38 produced a bisurfactant possess ing the same fragmentation pattern from mass spectra analysis of trehalose tetraester as already described (Tuleva et al, 2008).…”

Section: Discussionmentioning

confidence: 87%

Simultaneous Biodegradation of Phenol and n-Hexadecane by Cryogel Immobilized Biosurfactant Producing Strain Rhodococcus wratislawiensis BN38

Hristov

Christova

Kabaivanova

et al. 2016

Polish Journal of Microbiology

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A b s t r a c tThe capability of the biosurfactantproducing strain Rhodococcus wratislawiensis BN38 to mineralize both aromatic and aliphatic xeno biotics was proved. During semicontinuous cultivation 11 g/l phenol was completely degraded within 22 cycles by Rhodococcus free cells. Immobilization in a cryogel matrix was performed for the first time to enhance the biodegradation at multiple use. A stable simultaneous hydrocarbon biodegradation was achieved until the total depletion of 20 g/l phenol and 20 g/l nhexadecane (40 cycles). The alkanotrophic strain R. wratislawiensis BN38 preferably degraded hexadecane rather than phenol. SEM revealed well preserved cells entrapped in the heterogeneous supermacroporous structure of the cryogel which allowed unhindered mass transfer of xenobiotics. The immobilized strain can be used in real conditions for the treatment of contaminated industrial waste water. 288of materials due to their unique heterogeneous open porous structure, which significantly increases the equilibrium sorption properties and allows unhindered diffusion of solutes, nanoparticles and microparticles. Usually, cryogels possess spongylike structure of huge pores (50-200 µm) containing free water surrounded by thin walls and, therefore, they are often used for immo bilization of enzymes and cells by entrapment inside the channels of interconnected pores (Lozinsky et al., 2003).Many bacterial strains have been isolated with the abilities to degrade nhexadecane and phenol sepa rately, which are often used to represent the aliphatic and aromatic pollutant (Yordanova et al., 2009; Abdel Megeed et al., 2010;Tambekar et al., 2012). However, few strains have been reported to have the dual abilities (Sun et al., 2012).This paper aims to report the first study on simul taneous biodegradation of nhexadecane and phenol by Rhodococcus wratislawiensis BN38 immobilized in hydroxypropylcellulose/poly (Nisopropylacrylamide) cryogel matrix. Experimental Materials and MethodsMicroorganism, media and cultivation. The R. wrati slawiensis BN38, employed in this study was isolated from soil polluted with hydrocarbons by a standard enrichment technique (Tuleva et al., 2008 O, 0.2 and phenol and nhexadecane at 500 mg/l, unless otherwise mentioned, was used. The pH of the medium was adjusted to 7.0. The MSM was solidified as MSM phenol agar by addition of 1.8% agar when necessary. Cultures grown on MSM agar supplemented with 500 mg/l phenol were used to inoculate 500 ml Erlenmeyer flasks, containing 100 ml liquid MSM. At each cycle MSM was supplemented to 100 ml with fresh sterile medium without cell transfer and only phenol was added. Cultures were incubated during the longterm semicontinuous biodegradation processes while shaking (120 rpm) at 29°C. Inocula with cell density (OD 610 nm) of 0.4 were employed in the processes of biodegradation. The pH values were 6.7-6.8 all the time, due to the buffering activity of the nutrient medium used.Bacterial growth was assessed by determination of the optical density (OD 610 nm) of t...

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“…Rhamnolipids are powerful natural emulsifiers, can reduce the surface tension of water, can be used to assist in the breakdown and removal of oil spills, and have well-defined roles in medical and agricultural fields due to their antibacterial, antimicrobial, and antifungal properties [57]. Rhamnolipids biosurfactants can be applied to several industrial sectors, including the handling of industrial emulsions, controlling oil spills, biodegradation, detoxification of industrial effluents, bioremediation of contaminated soil, and in the production of biocompatible cosmetic products [58][59].…”

Section: Applications Of Rhamnolipidsmentioning

confidence: 99%

The Definition, Preparation and Application of Rhamnolipids as Biosurfactants

Kamal-Alahmad¹

2015

IJNFS

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Rhamnolipids mainly are produced by Pseudomonas aeruginosa and another microorganism that have been found to have good surface activity. Rhamnolipids are used in various application areas, including environmental, health, food, cosmetic, oil industries, pharmaceuticals and environmental bio remediation particularly in enhanced oil recovery (EOR) and cleaning of oil spills. Many kinds of bio surfactant such as, rhamnolipids are already being used in industry but it is important to develop indigenous technology for the production of rhamnolipids produced by the microorganisms of local origin which would be more suitable for the application to that specific areas. Rhamnolipids have several beneficial uses: they are easily degradable, nontoxic, nonmutagenic, and have the highest surface-tension-reduction index of any surface-tension reducing agent currently in use. In this review, I summarize the definition, preparation, properties, and the application in different areas especially in food and agriculture, and industrial potential of rhamnolipids, as the next generation of biosurfactants.

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Effects of rhamnolipids on cell surface hydrophobicity of PAH degrading bacteria and the biodegradation of phenanthrene

Cited by 123 publications

References 39 publications

Alteration in cell surface properties of Burkholderia spp. during surfactant-aided biodegradation of petroleum hydrocarbons

Alteration in cell surface properties of Burkholderia spp. during surfactant-aided biodegradation of petroleum hydrocarbons

Simultaneous Biodegradation of Phenol and n-Hexadecane by Cryogel Immobilized Biosurfactant Producing Strain Rhodococcus wratislawiensis BN38

The Definition, Preparation and Application of Rhamnolipids as Biosurfactants

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