Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2011.12.048 View full text
Jace Fogle, Nina Mohan, Eric Cheung, Josefine Persson

Abstract: The effects of resin ligand density on cation exchange chromatography performance in preparative monoclonal antibody purification processes were evaluated. A set of agarose-based cation exchange resins spanning a relatively wide range of ligand densities was tested using three different monoclonal antibodies with unique impurity profiles. Experiments were performed in bind-and-elute mode using gradient elution at both intermediate protein loadings and near saturation capacity. Ligand density did not affect cle…

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