Effects of pulp and paper mill effluent extracts on liver anaerobic and aerobic metabolic enzymes in rainbow trout

Orrego, Rodrigo; Pandelides, Zacharias; Guchardi, John; Holdway, Douglas A.

doi:10.1016/j.ecoenv.2010.10.042

Cited by 20 publications

(10 citation statements)

References 32 publications

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“…Furthermore, few estrogenic effects were observed by the in vitro yeast estrogen screening assay, and these were only observed in Brazilian mill nonpolar SPE extracts . This lack of observed estrogenic effects differs from recent studies using in situ exposures and previous studies using similar intraperitoneal injections of SPE extracted effluents where not only estrogenic effects were prevalent but also the presence of compounds capable of inducing androgen aromatization enzymes leading to a final estrogenic effect was established . The reasons for these discrepancies are unclear and need to be understood to formulate best management practices for mills that result in effluents that have minimal or no effect on fish reproduction.…”

Section: Introductionmentioning

confidence: 71%

Evaluating the potential of effluent extracts from pulp and paper mills in Canada, Brazil, and New Zealand to affect fish reproduction: Estrogenic effects in fish

Orrego

Milestone

Hewitt

et al. 2016

Enviro Toxic and Chemistry

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The authors examined the potential of pulp mill effluent from pulp-producing countries (Canada, Brazil, New Zealand) to affect fish reproduction. Specifically, the estrogenic effects in juvenile rainbow trout (Oncorhynchus mykiss) pulse-exposed to 11 different mill effluent extracts (intraperitoneal injections of solid-phase extraction-dichloromethane nonpolar fraction). The results indicated that effluent extracts were estrogenic in juvenile trout irrespective of the gender, as reflected by increasing level of plasma vitellogenin (VTG; Brazil > New Zealand > Canada). Despite the high variability observed among mills, differences in VTG levels were related to the type of mill process (kraft > elementary chlorine-free kraft > thermomechanical pulping). Moreover, effluent treatments did not appear to significantly decrease VTG induction. A consistent estrogenic effect was observed in those mills that process a combination of feedstocks (softwood and hardwood), with the highest increase in VTG related to eucalyptus feedstock. The results demonstrate significant estrogenic effects of pulp mill effluents on chronically exposed juvenile trout, suggesting that in vivo metabolic activation of precursors is necessary to cause the observed increases in VTG levels. This molecular estrogenic response provides a useful starting point for predicting population-level impacts through the adverse outcome pathway methodology. Environ Toxicol Chem 2017;36:1547-1555. © 2016 SETAC.

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Section: Introductionmentioning

confidence: 71%

Evaluating the potential of effluent extracts from pulp and paper mills in Canada, Brazil, and New Zealand to affect fish reproduction: Estrogenic effects in fish

Orrego

Milestone

Hewitt

et al. 2016

Enviro Toxic and Chemistry

Self Cite

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“…These high LDH levels could be an indication of metabolic failure of the liver (Orrego et al 2010) due to the exposure of M. gayi gayi to pollution sources (industrial waste and domestic sewage) associated with the Biobío river and the coastal zones of centralsouthern Chile (e.g., Parra et al 1993, Riveros et al 1996, Muñoz 2002, also demonstrated by other studies (Monteiro et al 2007, Orrego et al 2011. Further research is needed to explore this hypothesis.…”

Section: Discussionmentioning

confidence: 61%

Aerobic and anaerobic enzyme activity in the hake Merluccius gayi gayi related to the Oxygen Minimum Zone off central-southern Chile

Saavedra¹,

Quiñones²,

González³

2016

Rev. biol. mar. oceanogr.

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“…In contrast to Christianson-Heiska et al (2008), Teresaki et al (2009) showed that anti-estrogenicity of RAs such as DHAA is not accounted by competitive binding to ER. Additional information on the anti-estrogenic action of DHAA is suggested to occur indirectly through liver damage; the site for vitellogenin synthesis (Orrego et al 2011). However, a more recent study opposed that claim, and suggested the disruption of estradiol metabolism by DHAA as an alternative explanation (Pandelides et al 2014).…”

Section: Industrial Sediment Ppm: Responses Of Estrogenic Biomarkersmentioning

confidence: 95%

Probing the xeno-oestrogenic impact of sediment cores contaminated by the pulp and paper industry: induction of aromatase cyp19a1b in late larval zebrafish

Sahoo

Oikari

2014

J Soils Sediments

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To assess xenoestrogenicity, the 20 days-post-fertilization zebrafish (20dpfZF) life-stage was experimentally exposed to known model chemicals as well as to environmentally contaminated samples. From a selection of key responsive genes, molecular responses associated with the steroidogenic impact (brain aromatase, cyp19a1b and vitellogenin isoform 1, vtg1) were used to assess the endocrinic modulation by xenoestrogenic samples. This work showed diverse in vivo responses by the 20dpfZF, from pure chemicals (bisphenol A, BPA; 17 -ethinylestradiol, EE2; nonylphenol, NP; octylphenol, OP), and their mixture combinations, and to industrially contaminated sediment samples. Gene expression analysis of cyp19a1b and vtg1 in exposed 20dpfZF showed the estrogenic potencies of chemicals in the order: EE2 > OP > BPA > NP. Transcriptional up-regulation of vtg1 in early juvenile fish exposed to mixtures (BPA + NP + OP), relative to individual concentrations, suggest in minimum the additive in vivo effect of a mixture of very low sublethal doses of single xenoestrogens. Complementary expression of cyp19a1b and vtg1 in 20dpfZF, exposed to artificially-spiked sediment with EE2 established the estrogenresponsiveness of the model. In contrast, the lack of simple estrogenic or antiestrogenic trait of correspondence in biomarker transcription was evident from exposure to two types of industrially contaminated sediments, suggesting common non-linearity of in vivo response by the model. The results also highlight the applicability of 20dpfZF in the assessment of remediation and monitoring of historically polluted sites. This study showed that the 20dpfZF addressed the regulatory requirement of a multi-purpose and cost-effective ecotoxicological model capable of in vivo genomic assessment of endocrineactive substances in aquatic samples.

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Effects of pulp and paper mill effluent extracts on liver anaerobic and aerobic metabolic enzymes in rainbow trout

Cited by 20 publications

References 32 publications

Evaluating the potential of effluent extracts from pulp and paper mills in Canada, Brazil, and New Zealand to affect fish reproduction: Estrogenic effects in fish

Evaluating the potential of effluent extracts from pulp and paper mills in Canada, Brazil, and New Zealand to affect fish reproduction: Estrogenic effects in fish

Aerobic and anaerobic enzyme activity in the hake Merluccius gayi gayi related to the Oxygen Minimum Zone off central-southern Chile

Probing the xeno-oestrogenic impact of sediment cores contaminated by the pulp and paper industry: induction of aromatase cyp19a1b in late larval zebrafish

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