Effects of pentoxifylline and platelet activating factor on sperm DNA damage

Ünsal, Evrim; Turan, Volkan; Aktuna, Süleyman; Hürdağ, Canan; Bereketoglu, Gamze; Canillioglu, Yasemin; Baltaci, A.; Özcan, Sarp; Karayalçın, Rana; Batırbaygil, Hande; Baltacı, Volkan

doi:10.1016/j.ejogrb.2015.12.016

Cited by 20 publications

(13 citation statements)

References 31 publications

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“…15 An important technique used to differentiate the live spermatozoa is the use of pentoxifylline. The concern about the chromatin damage caused by pentoxifylline use, as described by Unsal et al 10 make this study very useful for assisted reproduction routines. Accordingly to our results, the use of pentoxifylline in 1.5mM concentration does not affect the genetic material of the patient, even when DNA fragmentation rates are higher.…”

Section: Discussionmentioning

confidence: 91%

“…With the inhibition of the phosphodiesterase, there is an increase in the available AMPc levels, resulting in larger amount of active PK-A, which leads to a gain of sperm motility. 9 However, a question raised by Unsal et al 10 is the chance of DNA damage induced by pentoxifylline. The presence of fragmentation in the chromatin may have a negative impact on fertility rates, particularly in ICSI.…”

Section: /7mentioning

confidence: 99%

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Activation of the mobility of human spermatozoa with the use of pentoxifylline: effects on spermal DNA

Dutra¹,

Silva²,

Lazzari³

et al. 2017

Hum. Reprod. Arch.

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Introduction: 50% of pregnancy failure is due male factors. One of these factors is the absence of spermatozoa in the ejaculate; in these cases the patient is submitted to epididymal or testicular sperm aspiration. The spermatozoa acquired from these technics usually are immobile, what makes necessary the use of pentoxifylline, which activates sperm movement, to distinction of viable spermatozoa to ICSI. Objectives: To analyze if a low concentration of pentoxifylline causes spermatic chromatin damages. Materials and Methods: Sperm motility activation was tested in different concentrations of pentoxifylline. After the screening of doses, the lowest effective concentration was choose to analyze the chromatin damage rate. 15 samples of fresh sperm were tested for DNA damage with HALOSPERM  Kit. Blades' analyses were made in bright field microscopy. The fragmented DNA was settle by the absence of chromatin dispersion halo or small halo. Results: The lowest and effective pentoxifylline dose was 1.5 mM. There was no significant difference on chromatin damage rate between study and control groups (p = 0.55). Conclusion: The use of pentoxifylline at 1.5 mM does not affect the sperm DNA.

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Section: Discussionmentioning

confidence: 91%

Section: /7mentioning

confidence: 99%

Activation of the mobility of human spermatozoa with the use of pentoxifylline: effects on spermal DNA

Dutra¹,

Silva²,

Lazzari³

et al. 2017

Hum. Reprod. Arch.

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“…Ap4A also serves as an alarmone for heat stress in E. coli (24). Whether Ap4A plays a role in S. aureus DNA damage caused by PLTs is not yet clear; however, it has been reported that the PLT-activating factor (PAF) derived from PLT transfusion, which is used to increase sperm motility in embryology laboratories, was proven to damage the DNA of sperm (25). In another study, PAF could also arrest the proliferation of human mast cells.…”

Section: Discussionmentioning

confidence: 99%

Platelets directly regulate DNA damage and division ofStaphylococcus aureus

Zhang

et al. 2018

FASEB j.

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Platelets (PLTs) are classically used in the clinical setting to maintain hemostasis. Recent evidence supports important roles for PLTs in host inflammatory and immune responses, and PLT-rich plasma has been demonstrated to inhibit the growth of bacteria in vitro and in vivo; however, few studies have examined whether PLTs can inhibit bacterial growth directly, and related mechanisms have not been elucidated further. Accordingly, in this study, we evaluated the effects of PLTs on bacterial growth. We washed and purified PLTs from peripheral blood, then confirmed that PLTs significantly inhibited the growth of Staphylococcus aureus when cocultured in vitro. Moreover, PLTs damaged DNA and blocked cell division in S. aureus. During coculture, PLT-derived TGF-β1 was dramatically down-regulated compared with that in PLT culture alone, and the addition of TGF-β1 to the coculture system promoted the inhibition of PLTs on S. aureus. Analysis of a murine S. aureus infection model demonstrated that the depletion of PLTs exacerbated the severity of infection, whereas the transfusion of PLTs alleviated this infection. Our observations demonstrate that PLTs could directly inhibit the growth of S. aureus by damaging DNA and blockage cell division, and that PLT-derived TGF-β1 may play an important role in this machinery.-Xu, J., Yi, J., Zhang, H., Feng, F., Gu, S., Weng, L., Zhang, J., Chen, Y., An, N., Liu, Z., An, Q., Yin, W., Hu, X. Platelets directly regulate DNA damage and division of Staphylococcus aureus.

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“…There is no similar study that confirms these results. Although there are different studies that have concluded that some reagents such as PVP (Salian, Kalthur, Uppangala, Kumar, & Adiga, 2012), pentoxifylline and platelet‐activating factor (Unsal et al., 2016), and sodium nitroprusside (Santiso et al., 2012) can damage the DNA of spermatozoa in a short period of time, we do not know the exact mechanism causing the DNA fragmentation elevation. One of the critical materials in ELB is ammonium.…”

Section: Discussionmentioning

confidence: 95%

Application of erythrocyte lysing buffer (ELB) has detrimental effects on human sperm quality parameters, DNA fragmentation and chromatin structure

et al. 2020

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Over 70 million couples worldwide suffer from infertility (Ombelet et al., 2008). Approximately 1% of all infertile men have obstructive (OA) or nonobstructive azoospermia (NOA; Inhorn & Patrizio, 2015). Contributing to this statistic is the fact that azoospermia is characterised by a lack of spermatozoa in the ejaculated fluid (Fang et al., 2019). If viable testicular spermatozoa can be obtained from these patients, full developmental potential zygotes may be formed using the ICSI procedure (Fang et al., 2019; Shrem et al., 2019). Fortunately, tissue extracted from the testis contains spermatozoa and round germ cells, interstitial cells, Sertoli cells (Crabbé, Verheyen, Tournaye, & Van Steirteghem, 1997) and a high concentration of erythrocytes (Nagy, Verheyen, Tournaye, Devroey, & Van Steirteghem, 1997). According to commonly accepted testicular sperm preparation protocols, sperm suspension is exposed to erythrocyte lysing buffer (ELB) for 5-10 min. This preparation eliminates erythrocytes and produces a clean suspension of testicular spermatozoa. Therefore, it facilitates the search for sperm cells

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Effects of pentoxifylline and platelet activating factor on sperm DNA damage

Cited by 20 publications

References 31 publications

Activation of the mobility of human spermatozoa with the use of pentoxifylline: effects on spermal DNA

Activation of the mobility of human spermatozoa with the use of pentoxifylline: effects on spermal DNA

Platelets directly regulate DNA damage and division ofStaphylococcus aureus

Application of erythrocyte lysing buffer (ELB) has detrimental effects on human sperm quality parameters, DNA fragmentation and chromatin structure

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