Effects of Panax Ginseng Alcoholic Extract on Histomorphometric Changes of Ovaries in Offspring Rats from Diabetic Mothers

Zare, Afshin; Khaksar, Zabihollah

doi:10.13005/bbra/2027

Cited by 1 publication

(2 citation statements)

References 22 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Zare et al . reported elevation of both numbers and size of primordial follicles in ovaries of three-month-old female rat infants born to diabetic mothers, which was treated with ginseng extract [ 22 ]. A recent study has reported the disappearance of ovarian cysts after a 14-day oral regimen of Korean red ginseng extract in an animal model [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Ginseng alleviates folliculogenesis disorders via induction of cell proliferation and downregulation of apoptotic markers in nicotine-treated mice

et al. 2022

View full text Add to dashboard Cite

Background Ginseng is a powerful phytoestrogen with high antioxidant properties. Objective This study aimed to evaluate the effect of Panax Ginseng (PG) on folliculogenesis, proliferation, and apoptosis in the ovary impaired by nicotine. Methods Forty adult mice were divided into five groups. Control, sham, and nicotine groups, and co-treated groups of nicotine and ginseng in doses of 0.5 and 1 g/kg. Folliculogenesis was assessed via histopathology and serum evaluation of estradiol, progesterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) by ELISA. Lipid peroxidation and antioxidant enzyme activities both in homogenate tissue and serum were assayed by colorimetric analysis. Apoptotic markers of cytochrome c (Cyt c), Bax, and Bcl-2 were evaluated by RT-PCR. Proliferative index was studied by the Ki-67 immunostaining procedure. Results In comparison to the control or sham groups, nicotine significantly reduced the levels of FSH, LH, and estradiol hormones. An insignificant reduction was observed in the progesterone hormone. Nicotine reduced all healthy follicle numbers, except primordial (P = 0.001). Malondialdehyde (MDA) was increased in tissue and serum in the nicotine group (P = 0.01). Serum catalase (CAT) and enzymatic activity of superoxide dismutase (SOD) both were reduced in tissue and the serum, in the nicotine group. Nicotine induced a reduction in the proliferative indexes of granulosa and theca cells in pre-antral and antral follicles (P = 0.001). However, its effect on the proliferative index of stroma cells was not significant. Apoptotic markers were elevated in the nicotine group (P = 0.001). Co-treatment with ginseng elevated all sex hormones, increased healthy follicles, and reduced tissue or serum lipid peroxidation, compared with the nicotine group (p < 0.05). Co-Treatment with ginseng also reduced the expression of apoptotic markers and increased the proliferative indexes in granulosa and theca cells in pre-antral and antral follicles and also in stroma cells, in comparison to the nicotine group (P = 0.001). All above-mentioned alterations following treatment with ginseng were remarkable, especially in the dose of 1 g/kg. Conclusion This study showed ginseng protects folliculogenesis via alteration of hypothalamic- pituitary–gonadal (HPG) axis, induction of proliferation in ovarian somatic cells, reduction of lipid peroxidation, and downregulation of apoptotic markers in the mouse ovary, treated with nicotine.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Ginseng is a powerful phytoestrogen with high antioxidant properties. There are various reports about the beneficial effects of ginseng on various disorders of the systems such as cardiovascular, endocrine, immune system, menopausal symptoms, and reproduction [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%