Effects of orthopaedic wear particles on osteoprogenitor cells

Goodman, Stuart B.; Ma, Ting; Chiu, Richard; Ramachandran, Ravi P.; Smith, Robert L.

doi:10.1016/j.biomaterials.2006.08.023

Cited by 117 publications

(116 citation statements)

References 30 publications

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“…13 Additionally, it has been shown that wear particles adversely affect the differentiation, proliferation, and osteogenicity of osteoblast precursors. [14][15][16] However, in our study, the migrated exogenous MC3T3 cells correlated with a significant local increase in BMD and cortical thickness. The increased BMD is a combined effect of resident osteoblasts and migrated preosteoblasts that were able to differentiate into mature osteoblasts.…”

Section: Fritton Et Alcontrasting

confidence: 64%

“…13 However, wear particles adversely affect the health of osteoblasts and their precursors by impairing their proliferation, differentiation, and osteogenicity. [14][15][16] Ultimately, maintaining the number and health of osteoblasts could help slow or reverse the progression of osteolysis. To this end, it has recently been shown that polymethyl methacrylate particle-conditioned media induced migration of MSCs, an effect that was blocked by neutralizing the macrophage inflammatory protein-1a (MIP-1a), a chemokine also known to attract monocyte/macrophages.…”

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confidence: 99%

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Exogenous MC3T3 Preosteoblasts Migrate Systemically and Mitigate the Adverse Effects of Wear Particles

Fritton

Ren

Gibon

et al. 2012

Tissue Engineering Part A

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Understanding how relevant cell types respond to wear particles will reveal new avenues for treating osteolysis following joint replacements. In this study, we investigate the effects of ultrahigh molecular weight polyethylene (UHMWPE) particles on preosteoblast migration and function. We infused UHMWPE particles or saline into the left femur of mice and injected luciferase-expressing preosteoblasts (MC3T3 cells) into each left ventricle. Bioluminescence imaging (BLI) confirmed systemic administration of MC3T3 cells. BLI throughout the 28-day experiment showed greater MC3T3 migration to the site of particle infusion than to the site of saline infusion, with significant differences on days 0, 4, and 6 ( p £ 0.055). Immunostaining revealed a greater number of osteoblasts and osteoclasts in the particle-infused femora, indicating greater bone turnover. The bone mineralization of the particle-infused femora increased significantly when compared to saline-infused femora (an increase of 146.4 -27.9 vs. 12.8 -8.7 mg/mL, p = 0.008). These results show that infused preosteoblasts can migrate to the site of wear particles. Additionally, as the migrated cells were associated with increased bone mineralization in spite of the presence of particles, increasing osteoblast recruitment is a potential strategy for combating bone loss due to increased osteoclast/macrophage number and decreased osteoblast function.

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Section: Fritton Et Alcontrasting

confidence: 64%

mentioning

confidence: 99%

Exogenous MC3T3 Preosteoblasts Migrate Systemically and Mitigate the Adverse Effects of Wear Particles

Fritton

Ren

Gibon

et al. 2012

Tissue Engineering Part A

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“…Therefore, the association between particles and osteolysis represents a doseresponse relationship (Wilkinson et al, 2005) Interestingly, osteoblasts also can phagocyte small particles, causing potential adverse effects on viability, proliferation and function of osteoblast as well as on osteoclasts (Goodman et al, 2006;Lohmann et al, 2000). PE, PMMA or metallic particles reduce osteoblasts differentiation of bone marrow osteoprogenitor cells (Chiu et al, 2006), expression of collagens by osteoblasts (Vermes et al, 2001;Vermes et al, 2000), osteoblast viability by inducing apoptosis (Pioletti et al, 2002) characterized with decreased production of matrix, alkaline phosphatase and TGF-by these cells (Dean et al, 1999). As for macrophages, such suppressive effects are also likely dependent on particle size, composition and dosage: different particle types can differentially affect osteoblast function (Lohmann et al, 2002).…”

Section: Wear Particle Debris -The Main Cause Of Periprosthetic Osteomentioning

confidence: 99%

“…For example, particles directly inhibit cell viability and proliferation, in addition to down-regulating the mRNA and protein level of bone formation markers. Particles less than 5 μm can also undergo phagocytosis by mature osteoblasts (Goodman et al, 2006), leading to potential adverse effects on cellular viability, proliferation and function. Along with particle size, composition and dosage can also effect these parameters (Lohmann et al, 2002).…”

Section: Impaired Osteogenesis As An Inflammatory Reaction In Peripromentioning

confidence: 99%

Inflammatory Periprosthetic Bone Loss

Lee¹,

Purdue²,

Nam³

2012

Inflammatory Diseases - Immunopathology, Clinical and Pharmacological Bases

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“…1 Recent studies have shown that wear particles also contribute to osteolysis by impairing the osteogenic potential of osteoprogenitors and mesenchymal stem cells. [2][3][4][5][6][7] Because osteolysis depends on the equilibrium between bone degradation and bone regeneration, an inhibitory effect on osteoblast production can tip the balance in the direction of accelerated bone loss.…”

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confidence: 99%

Polymethylmethacrylate particles inhibit osteoblastic differentiation of MC3T3‐E1 osteoprogenitor cells

Chiu

Smith

et al. 2008

Journal Orthopaedic Research

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Orthopedic wear debris has been implicated as a significant inhibitory factor of osteoblast differentiation. Polymethylmethacrylate (PMMA) particles have been previously shown to inhibit the differentiation of osteoprogenitors in heterogeneous murine marrow stromal cell cultures, but the effect of PMMA particles on pure osteoprogenitor populations remains unknown. In this study, we challenged murine MC3T3-E1 osteoprogenitor cells with PMMA particles during their initial differentiation in osteogenic medium. MC3T3-E1 cultures challenged with PMMA particles showed a gradual dose-dependent decrease in mineralization, cell number, and alkaline phosphatase activity at low particle doses (0.038-0.150% v/v) and complete reduction of these outcome parameters at high particle doses (!0.300% v/v). MC3T3-E1 cultures challenged with a high particle dose (0.300% v/v) showed no rise in these outcome parameters over time, whereas cultures challenged with a low particle dose (0.075% v/v) showed a normal or reduced rate of increase compared to controls. Osteocalcin production was not significantly affected by particles at all doses tested. MC3T3-E1 cells grown in conditioned medium from particle-treated MC3T3-E1 cultures showed a significant reduction in mineralization only. These results indicate that direct exposure of MC3T3-E1 osteoprogenitors to PMMA particles results in suppression of osteogenic proliferation and differentiation. ß

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Effects of orthopaedic wear particles on osteoprogenitor cells

Cited by 117 publications

References 30 publications

Exogenous MC3T3 Preosteoblasts Migrate Systemically and Mitigate the Adverse Effects of Wear Particles

Exogenous MC3T3 Preosteoblasts Migrate Systemically and Mitigate the Adverse Effects of Wear Particles

Inflammatory Periprosthetic Bone Loss

Polymethylmethacrylate particles inhibit osteoblastic differentiation of MC3T3‐E1 osteoprogenitor cells

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