Effects of oral administration of Garcinia dulcis flower extract on arterial blood pressure and renal excretory functions in rats

Abstract

Intravenous infusion of either camboginol or morelloflavone from Garcinia dulcis (GD) exerted diuretic, hypotensive, and vasorelaxant effects in either normotensive or hypertensive rats. This study aims to investigate the effects of GD flower extract on arterial blood pressure (ABP) and renal excretory functions. Male Wistar rats (8-weekold) were divided into 4 groups (group I-IV, n = 6 each) in both acute and sub-chronic protocols. The GD extract was orally administrated to group II-IV at the dose of 50, 100, or 200 mg/kg, respectively, while group I served as vehicle control. The oral administration was performed before the experiment in acute protocol and daily for 2 weeks in the sub-chronic protocol. The ABP and renal excretory functions were measured in the anesthetized rats. The levels of fasting blood glucose (FBG), plasma lipid profiles, and liver enzymes were evaluated in the sub-chronic experiment along with liver histology. The results showed that acute administration of GD extract significantly decreased ABP but increased renal blood flow, glomerular filtration rate, urine flow rate, osmolar clearance, and negative free water clearance when compared with the control. In the sub-chronic protocol, the GD extract significantly decreased ABP but did not alter the renal excretory functions. The plasma levels of FBG, lipid profiles, liver enzymes, and the histology of liver were not changed. It is concluded that acute oral administration of GD extract possessed hypotensive and diuretic effects whereas the sub-chronic treatment of GD showed hypotensive effect and no alterations in liver function, FBG, and plasma lipid profiles.

Garcinia dulcis Flower Extract Alters Gut Microbiota and Fecal Metabolomic Profiles of 2K1C Hypertensive Rats

“…GD flowers were collected from Songkhla province, Thailand. GD extraction and purification procedures were previously described by Thongsepee et al, 2020 [ 20 ]. The major chemical components of the GD flower extract were morelloflavone and camboginol.…”

Camboginol and Morelloflavone from Garcinia dulcis (Roxb.) Kurz Flower Extract Promote Autophagic Cell Death against Human Glioblastoma Cells through Endoplasmic Reticulum Stress

“…Camboginol and morelloflavone were identified as major bioactive components in several parts of Garcinia spp. ( Thongsepee et al, 2020 ). Morelloflavone was presented as a higher proportion in the extract than camboginol (unpublished data).…”

“…The extraction and purification of the G. dulcis flower were previously described ( Deachathai et al, 2006 ). The hexane-insoluble fraction, composed of camboginol and morelloflavone, was used in this study as previously described ( Deachathai et al, 2006 ; Thongsepee et al, 2020 ). The G. dulcis fraction was dissolved in dimethyl sulfoxide (DMSO, Sigma-Aldrich Pty Ltd., Darmstadt, Germany) and warmed at 40°C before use.…”

“…Camboginol (plant benzophenone) and morelloflavone (biflavonoids) are found in the insoluble hexane extract of G. dulcis flowers, as previously reported ( Deachathai et al, 2006 ). The oral administration of extracts from the flower of G. dulcis has been found by previous studies to reduce hypertension and promote diuretics in renovascular hypertensive rats ( Thongsepee et al, 2020 ; Thongsepee et al, 2022 ).…”

“…The extraction and purification of the G. dulcis flower were previously described (Deachathai et al, 2006). The hexane-insoluble fraction, composed of camboginol and morelloflavone, was used in this study as previously described (Deachathai et al, 2006;Thongsepee et al, 2020).…”