Effects of occupational environmental controls and work management on chromosomal damage in dental technicians in Japan

Ishikawa, Shinya; Ishikawa, Hitoshi; Shindo, Taku; Yoshida, Takafumi; Shimoyama, Yasuaki; Satomi, Takashi; Fujii, Satoshi; Hamamoto, Yoshioki; Iino, Mitsuyoshi; Fukao, Akira

doi:10.1016/j.ijheh.2012.02.005

Cited by 15 publications

(11 citation statements)

References 49 publications

(45 reference statements)

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“…The baseline MN frequency in the general Japanese population has been reported to be 4–7 per 1000 BNCs [16, 17], which is lower than our observations. This could be due to differences in the protocols and slide preparation methods employed.…”

Section: Discussioncontrasting

confidence: 95%

Possible benefits of tomato juice consumption: a pilot study on irradiated human lymphocytes from healthy donors

Nakamura

Itaki

Saito

et al. 2017

Nutr J

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BackgroundReactive oxygen species (ROS) mediate much of the DNA damage caused by ionizing radiation. Among carotenoids, lycopene and β-carotene, present in tomato juice, are known to be strong radical scavengers. The aim of the study was to investigate the effect of tomato juice intake on the levels of DNA damage and oxidative stress in human whole blood induced by in vitro exposure to X-rays.MethodsTen healthy adults were asked to drink 190 g of tomato juice, containing 17 mg lycopene and 0.25 mg β-carotene, per day for 3 weeks and then refrain from drinking it for 3 weeks. Peripheral whole blood samples were collected before and after the intake period of tomato juice and after the washout period. The blood samples were exposed in vitro to X-ray doses of 0, 0.1, 0.5, and 2 Gy. Cytogenetic damage was measured using the cytokinesis-block micronucleus (CBMN) assay and the dicentrics (DIC) assay. The level of oxidative stress was determined using serum 8-oxo-7, 8-dihydro-2-deoxyguanosine (8-oxo-dG) and plasma reactive oxygen metabolite-derived compounds (d-ROMs). The concentration of carotenoids in plasma was measured at the three time points.ResultsThe levels of 8-oxo-dG tended to decrease during the intake period and increase during the washout period. A non-significant inverse correlation was noted between the plasma concentration of lycopene plus β-carotene and the level of 8-oxo-dG (P = 0.064). The radiation-induced MN and DIC frequencies increased in a dose-dependent manner, and when compared at the same dose, the MN and DIC frequencies decreased during the intake period compared with those at baseline and then increased during the washout period. The results suggest that continuous tomato juice consumption non-significantly decreases extracellular 8-oxo-dG, d-ROMs, and MN. Tomato juice intake had minimal or no effect on radiation-induced 8-oxo-dG and d-ROMs. For most radiation doses, continuously tomato juice intake lowered the levels of MN and DIC.ConclusionTomato juice consumption may suppress human lymphocyte DNA damage caused by radiation, but further examination is required.Trial registration 2014-001 and 2014-R06.

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Section: Discussioncontrasting

confidence: 95%

Possible benefits of tomato juice consumption: a pilot study on irradiated human lymphocytes from healthy donors

Nakamura

Itaki

Saito

et al. 2017

Nutr J

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“…This phenomenon showed that a dental technician is one of occupations that is risky for metal hazard such as Cobalt, Nickel and Chromium exposure. This result supports the finding of other research such as research in the Northern Jordan [14], Ankara [15] and Japan [16] showing the occupational health hazard of being dental technicians. We know that, metal interfere with many cellular reactions.…”

Section: Discussionsupporting

confidence: 91%

“…Some of them were diagnosed as having Parkinson disease and postural tremor [11]. The exposure to Cobalt, Nickel and Chromium has been also attributed to elevated levels of those metals in blood and urin [8], [14]- [16].…”

Section: Introductionmentioning

confidence: 99%

Effects of Occupational Environmental Controls on the Level of Co, Ni and Cr among Dental Technicians

Hariyani¹,

Berniyanti²,

Setyowati³

2015

IJESD

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Abstract-Dental laboratory technicians may be exposed to metal alloys that are used in the production of crowns, bridges and removable partial dentures. The aim of this study was to assess whether dental technicians are occupationally exposed to Cobalt, Nickel and Chromium by analyzing the concentration of Cobalt, Nickel and Chromium in the blood level and to investigate the effects of occupational environmental controls associated with dental prostheses production in dental laboratories in East Java Indonesia. Result found shows that the concentration of Cobalt, Nickel and Chromium in the blood level of dental technicians are higher than the certified reference material level recommended. The concentration of Cobalt, Nickel and Chromium in the dental technicians' blood level are 27 µg/L, 37 µg/L and 117 µg/L respectively. Although 50% of dental laboratories surveyed had ventilator and air dust respirator, almost all of the technicians did not do personal occupational environmental controls correctly.

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“…The sample size in that study does not allow detection of the association between professional exposure to MMA and the incidence of micronuclei in the buccal mucosa cells of dental technicians. Ishikawa et al evaluated the effects of professional environmental controls and work management on chromosomal damage in dental technicians. They observed that significant parameters affecting the MN frequency in peripheral lymphocytes of dental technicians were hand‐washing (β = –0.304, p < 0.01), duration of employment (β = 0.509, p < 0.01), and workplace (β = 0.235, p < 0.05).…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic Biomonitoring of Dental Technicians: A Cross‐Sectional Study

Tadin

Gavić

Jurković

et al. 2018

Journal of Prosthodontics

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Purpose This is a cross‐sectional study to assess the incidence of micronuclei and other nuclear morphological changes in buccal epithelial cells of dental technicians. Materials and Methods The study was conducted on 45 dental technicians versus 2 control groups: 50 dentists and 50 dental assistants. DNA damage was analyzed in exfoliated buccal mucosa cells by micronucleus assay. The differences in the frequency of detected types of cytogenetic damage between experimental groups were analyzed by applying 2‐way ANOVA with Tukey's HSD post‐hoc test. Results Dentists and dental assistants have significantly lower incidence of micronucleated cells than technicians (mean ± SD: 0.68 ± 0.74, 0.58 ± 0.81, and 1.58 ± 2.07; p = 0.031 and p = 0.015, respectively), and this trend also holds for karyolitic cells (0.10 ± 0.30, 0.20 ± 0.49, and 1.42 ± 1.25; p < 0.001 and p < 0.001, respectively), condensed chromatin (0.16 ± 0.37, 0.14 ± 0.35, and 0.76 ± 0.98; p < 0.001 and p < 0.001, respectively), and pyknotic cells (0.04 ± 0.20, 0.08 ± 0.27, and 0.96 ± 1.24; p < 0.001 and p < 0.001, respectively). Conclusion Cytogenetic biomarkers in dental technician buccal mucosa are increased compared with control groups. This increase may be associated with more extensive exposure to potentially harmful components of the materials used in everyday dental practice.

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Effects of occupational environmental controls and work management on chromosomal damage in dental technicians in Japan

Cited by 15 publications

References 49 publications

Possible benefits of tomato juice consumption: a pilot study on irradiated human lymphocytes from healthy donors

Possible benefits of tomato juice consumption: a pilot study on irradiated human lymphocytes from healthy donors

Effects of Occupational Environmental Controls on the Level of Co, Ni and Cr among Dental Technicians

Cytogenetic Biomonitoring of Dental Technicians: A Cross‐Sectional Study

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