We used DPPH scavenging assays to study the antioxidant activity of three native Polish species of blackberry leaves (Rubus kuleszae Ziel., R. fabrimontanus (Sprib.) Sprib. and R. capitulatus Utsch.). All the studied extracts (methanolic, water, methanolic-water) showed high DPPH free radical scavenging activity (IC50 450.0-186.0 μg/ml). The most effective of the studied species was Rubus kuleszae. Total content of phenolic compounds (70.50-136.04 mg GAE/g) and phenolic acids (14.70-38.26 mg CAE/g) was determined spectrophotometrically. Antioxidant activity correlated positively with total content of phenolic compounds and phenolic acids.K Ke ey y w wo or rd ds s: : Rubus, blackberry, leaf extract, antioxidant activity, DPPH, phenolic compounds, phenolic acids.ACTA BIOLOGICA CRACOVIENSIA Series Botanica 54/2: 32-38, 2012 DOI: 10.2478 PL ISSN 0001-5296 © Polish Academy of Sciences and Jagiellonian University, Cracow 2012
INTRODUCTIONThe genus Rubus L. (blackberries/raspberries) comprises almost 700 species, making it the largest genus of the Rosaceae family and one of the most diverse of the plant kingdom (Alice and Campbell, 1999;Zieliński, 2004). Some species such as R. idaeus and R. fruticosus are valuable fruit plants and also used in medicine. The leaves of Rubus plants are known to contain astringent, antibacterial and antifungal agents, recommended internally in diarrhoea and inflammation of the gastrointestinal tract. Leaf infusions are also used externally for skin lesions and ulcers, and as a rinse in infections of the oral cavity and throat. This use of leaves of Rubus species relies on their high content of hydrolyzing (gallo-and ellagitannins) and condensed (catechins) tannins, and numerous other polyphenols including flavonoids (quercetin and kaempferol derivatives), anthocyanins and phenolic acids, particularly gallic and ellagic acid (Thiem, 2003;Benvenuti et al., 2004;Gudej and Tomczyk, 2004;Patel et al., 2004;Byamukama et al., 2005;Mertz et al., 2007;Ali et al., 2012). Among many possible effects on biological processes in the human body, the antioxidant properties of phenolic compounds are the most important. Many data gathered in recent years indicate the participation of free radical processes in the emergence of such lifestyle diseases as atherosclerosis, heart attack, stroke, cancer, diabetes, senile cataracts and accelerated aging. The presence and distribution of numerous hydroxyl groups in the chemical structure of polyphenols make them excellent antioxidants. They are able to chelate transition metal ions, particularly those of iron and copper, which are involved in initiating free radical chain reactions. They also inhibit the activity of many enzymes participating in the formation of free radicals: for example, xanthine oxidase, responsible for the production of large amounts of reactive oxygen forms during reperfusion, and NADPH oxidase, responsible for the so-called respiratory burst (RiceEvans et al., 1996;Pietta, 2000, Villańo et al., 2007Li et al., 2008).Phytochemical studies of...