Effects of nicotine on proliferation and osteoblast differentiation in human alveolar bone marrow-derived mesenchymal stem cells

Quantitative analyses of bone composition in acetylcholine receptor M3R and alpha7 knockout mice

“…The effect of nicotine administration to osteoblasts is controversially discussed. Some studies reported that incubation of cultured osteoblasts with low level of nicotine resulted in an up-regulation of collagen-1 mRNA expression (Rothem et al, 2009) whereas others found the opposite: a decrease in col 1α1 synthesis after treatment with nicotine and no effect on the proliferation of osteoblasts (Kim et al, 2012). Since osteoblast express a whole panel of nAChR we decided to focus on only one receptor.…”

Sirt3–MnSOD axis represses nicotine-induced mitochondrial oxidative stress and mtDNA damage in osteoblasts

“…Furthermore, nicotine could also induce dysfunction and death of osteoblasts [3,4]. However, the underlying mechanism remains elusive.…”

“…In vivo studies have demonstrated that tobacco smoking is a major risk factor for osteoporosis [1,2]. Nicotine, one of the main compounds in cigarette, influences the biological activity, proliferation, and differentiation of osteoblasts [3][4][5]. In addition, nicotine can also suppress the osteoblast differentiation from human mesenchymal stem cell (MSC) [6].…”

The effect of nicotine and cotinine on human gingival fibroblasts attachment to root surfaces

“…Therefore, the present study examined in vitro the effects of nicotine and cotinine on HGFs attached to root surfaces. In cigarette smoke, nicotine induces adverse effects on the viability of cells derived from human periodontal ligament [12 -16] , and smoking seems to play a role in periodontal disease through the induction of inhibitory effects on some immune system functions [17] .…”

“…High concentrations of nicotine and cotinine (especially 5 mM) inhibit the proliferation of the attached cells 48 h after exposure. Kim et al [17] showed that the proliferation of mesenchymal stem cells increased at low concentrations of nicotine (1 -2 mM) and decreased significantly at 5 mM of nicotine. Dual effects of nicotine have also been reported by Rothem et al [18] , although at different concentrations of nicotine, on the proliferation of human osteosarcoma cells (MG63).…”