Effects of NGF and BDNF on baseline glutamate and dopamine release in the hippocampal formation of the adult rat

Paredes, Daniel; Granholm, Ann‐Charlotte; Bickford, Paula C.

doi:10.1016/j.brainres.2007.01.018

Cited by 38 publications

(29 citation statements)

References 54 publications

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“…Microdialysis and neurochemical measurements were performed as described (49,50). Guide shafts were inserted into the brains of 3-4 month old Fischer 344 male rats at coordinates AP Ϫ4.30 mm, L 4.0 mm and DV 1.0 mm with respect to Bregma, the midsagittal sinus and the brain surface (51).…”

Section: Methodsmentioning

confidence: 99%

“…Animals with probe misplacement (i.e., outside of CA1 region) were excluded (14 out of 52 total) before measurement of DA and its metabolites. DA, HVA, and DOPAC were measured immediately after collection as described previously (49,50), by comparing sample peak areas to external standards. Average DA baseline concentration was 0.9 Ϯ 0.04 nM, uncorrected for the 18% recovery rate of microdialysis probes.…”

Section: Methodsmentioning

confidence: 99%

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Neuregulin-1 regulates LTP at CA1 hippocampal synapses through activation of dopamine D4 receptors

Kwon

Paredes

González

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

129

161

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Neuregulin-1 (NRG-1) is genetically linked with schizophrenia, a neurodevelopmental cognitive disorder characterized by imbalances in glutamatergic and dopaminergic function. NRG-1 regulates numerous neurodevelopmental processes and, in the adult, suppresses or reverses long-term potentiation (LTP) at hippocampal glutamatergic synapses. Here we show that NRG-1 stimulates dopamine release in the hippocampus and reverses early-phase LTP via activation of D4 dopamine receptors (D4R). NRG-1 fails to depotentiate LTP in hippocampal slices treated with the antipsychotic clozapine and other more selective D4R antagonists. Moreover, LTP is not depotentiated in D4R null mice by either NRG-1 or theta-pulse stimuli. Conversely, direct D4R activation mimics NRG-1 and reduces AMPA receptor currents and surface expression. These findings demonstrate that NRG-1 mediates its unique role in counteracting LTP via dopamine signaling and opens future directions to study new aspects of NRG function. The novel functional link between NRG-1, dopamine, and glutamate has important implications for understanding how imbalances in Neuregulin-ErbB signaling can impinge on dopaminergic and glutamatergic function, neurotransmitter pathways associated with schizophrenia.depotentiation ͉ ErbB receptor ͉ plasticity ͉ schizophrenia ͉ clozapine T he trophic and differentiation factor NRG-1 and its receptors (ErbB2-4) are expressed in the developing nervous system and adult brain, including the hippocampus. NRG-1 is translated as a transmembrane protein and released in an activity-dependent manner (1). Initially, long-term NRG-1 signaling was shown to regulate neuronal expression of neurotransmitter receptor genes for glutamate, acetylcholine, and GABA (2-5). More recently, the tight association of the NRG-1 receptor ErbB4 with glutamate receptors at postsynaptic densities suggested that NRG-1 signaling could regulate synaptic function in a more acute fashion (6, 7). Consistent with this idea, we and others have shown that NRG-1␤ rapidly regulates glutamatergic (7-11) and cholinergic (12) synaptic function in the hippocampus and prefrontal cortex (PFC).Long term potentiation (LTP) and long term depression (LTD) at Schaeffer collateral-to-CA1 hippocampal synapses (SC-CA1) are believed to underlie complex cognitive processes such as learning and memory. At this synapse, postsynaptic NMDAR activation and increases in AMPAR excitatory postsynaptic currents (EPSCs) are necessary for LTP induction and expression, respectively. An additional mechanism that contributes to synaptic homeostasis at adult glutamatergic synapses is depotentiation (13,14). In acute hippocampal slices and in freely moving animals, LTP is reversed (depotentiated) by brief, subthreshold theta pulse stimulation (TPS) if delivered during a labile period shortly after LTP induction (14). In the amygdala, depotentiation correlates with fear extinction and requires AMPAR internalization (15). We recently reported that NRG-1␤ depotentiates early-phase LTP at hippocampal SC-CA1 synapses,...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Neuregulin-1 regulates LTP at CA1 hippocampal synapses through activation of dopamine D4 receptors

Kwon

Paredes

González

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

129

161

View full text Add to dashboard Cite

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“…NGFs have also been seen to interact with dopaminergic neurons thus facilitating dopamine release at synapses (74,75). Dopamine is the chief substrate for mesolimbic reward pathway.…”

Section: Role Of Ngfs In Maintenance Of Normal Behaviourmentioning

confidence: 99%

Nerve Growth Factor(s) Mediated Hypothalamic Pituitary Axis Activation Model in Stress Induced Genesis of Psychiatric Disorders

Kumar¹,

Kumar²,

Pareek³

et al. 2017

Preprint

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Apart from their established role in embryonic development Nerve Growth Factors (NGFs) have diverse functions in the nervous system. Their role in integration of physiological functioning of the nervous system is now attracting attention. In the present analysis, we propose a new paradigm about a novel role of NGFs. We hypothesize that NGFs play imperative role in maintaining psychological integrity of an individual as a biological system. This function may be mediated through HPA-axis-operated homeostatic mechanisms; stress induced disruption of which may lead to psychiatric disorders.

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“…The peak decay is shifted to the right, which is typically associated with alterations in dopamine transporter kinetics (Km) 3 . Finally, Figure 3C is a representative trace of the current versus time trace once the slice has been bathed in a 100 ng/mL brain-derived neurotrophic factor (BDNF) solution, which has been hypothesized to influence dopamine release dynamics 20,21 . From this representative trace, it can be seen that BDNF has the ability to enhance electrically-evoked dopamine release.…”

Section: Representative Resultsmentioning

confidence: 99%

Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry

Maina

Khalid

Apawu

et al. 2012

JoVE

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Extensive research has focused on the neurotransmitter dopamine because of its importance in the mechanism of action of drugs of abuse (e.g. cocaine and amphetamine), the role it plays in psychiatric illnesses (e.g. schizophrenia and Attention Deficit Hyperactivity Disorder), and its involvement in degenerative disorders like Parkinson's and Huntington's disease. Under normal physiological conditions, dopamine is known to regulate locomotor activity, cognition, learning, emotional affect, and neuroendocrine hormone secretion. One of the largest densities of dopamine neurons is within the striatum, which can be divided in two distinct neuroanatomical regions known as the nucleus accumbens and the caudate-putamen. The objective is to illustrate a general protocol for slice fast-scan cyclic voltammetry (FSCV) within the mouse striatum. FSCV is a well-defined electrochemical technique providing the opportunity to measure dopamine release and uptake in real time in discrete brain regions. Carbon fiber microelectrodes (diameter of~7 μm) are used in FSCV to detect dopamine oxidation. The analytical advantage of using FSCV to detect dopamine is its enhanced temporal resolution of 100 milliseconds and spatial resolution of less than ten microns, providing complementary information to in vivo microdialysis. Video LinkThe • There are numerous carbon fiber microelectrodes fabrication methods since most are made in-house. Typically what dictates the electrode fabrication details is the electrochemical technique that is applied to the electrode (e.g. amperometry vs. FSCV). For FSCV, microelectrodes can be made in-house using the following three-step procedure. For a more complete description of carbon fiber electrode fabrication, see a recent JOVE article 1 . However, note that the electrodes described below are cylindrical carbon fiber microelectrodes, which require fewer steps to fabricate versus the amperometric carbon fiber microelectrodes from the above-mentioned protocol. This simplified protocol does not require boiling the carbon fiber in acetone, fire-polishing the glass capillaries, or using epoxy to seal the glass-fiber junction.• Using vacuum suction aspirate a carbon fiber (diameter 7 μm; Goodfellow Oakdale, PA) into a borosilicate glass capillary with microfilament (length 10 cm, o.d. 1.2 mm, i.d. 0.68 mm; A-M systems, Carlsborg, WA).• Place the threaded capillary into the electrode puller (Narishige, Tokyo, Japan) where the capillary is pulled in half. Output settings for the electrode puller do vary from lab to lab. For reference, our output settings for the puller are 90.7 main magnet, 23.2 sub-magnet, and 53.4 for the heater. The output settings should be empirically refined to generate a glass taper that is approximately 4.4 mm in length, with a tight seal around the carbon fiber.• Under a microscope (Olympus, Tokyo, Japan), trim the carbon fiber (using a scalpel blade) extending from the glass tip allowing approximately 50-200 μm of the carbon fiber to protrude from the tightly sealed interface.• S...

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Effects of NGF and BDNF on baseline glutamate and dopamine release in the hippocampal formation of the adult rat

Cited by 38 publications

References 54 publications

Neuregulin-1 regulates LTP at CA1 hippocampal synapses through activation of dopamine D4 receptors

Neuregulin-1 regulates LTP at CA1 hippocampal synapses through activation of dopamine D4 receptors

Nerve Growth Factor(s) Mediated Hypothalamic Pituitary Axis Activation Model in Stress Induced Genesis of Psychiatric Disorders

Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry

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