Effects of N-acetylcysteine on Semen Parameters and Oxidative/Antioxidant Status

Çiftçi, Halil; Verit, Ayhan; Savaş, Murat; Yeni, Ercan; Erel, Özcan

doi:10.1016/j.urology.2009.02.034

Cited by 140 publications

(104 citation statements)

References 21 publications

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“…According to the literatures documented, as it has not been found any literature except for two studies (Çoyan et al [34] and Ari et al [35] ) regarding with LE and the other antioxidants investigated in the present study, the main theme of the experiment could not be done subjectspecific discussion in detail. However, in different number of mammalian species, analyzing the results of different or similar antioxidants with some studies [36,37] especially, motility results obtained in the present study is lower and in those of some others is similar [38,39] . In the same study, in the group added 2 mM LE subjective, CASA and progressive motility values were 81.3%, 57.9% and 31.0%, while in the group added 4 mM, these scores were 80.6%, 65.2% and 32.4% respectively.…”

Section: Discussionsupporting

confidence: 40%

Farklı Kriyoprotektanların Eritme Sonrası Arı Sperm Kalitesi Üzerine Etkileri

Alçay¹,

Üstüner²,

Çakmak³

et al. 2015

Kafkas Univ Vet Fak Derg

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The aim of this study was to determine the influence of different doses of L-Ergothioneine (LE), cystein and N-acetylcystein (NAC) on post-thawing semen parameters in rams. Ejaculates collected from four Tushin Rams were evaluated and pooled at 35°C. Semen samples were diluted with skim-milk based extender containing LE (5, 10 mM), Cystein (5, 10 mM), NAC (5, 10 mM) or not containing any antioxidant (control) and loaded to 0.25 ml French straws. Straws were cooled to 5°C for 2 h, frozen in liquid nitrogen vapour (aprox. -120°C) for 15 min and then being stored in liquid nitrogen until thawing process. Straws were thawed in water bath (37°C for 1 min). The percentages of motility, viability, abnormal acrosome, total abnormalities, membrane integrity (hypoosmatic swelling test, HOST) were statistically assessed. Also total antioxidant capacity (TAC) and total oxidatif stres (TOS) were evaluated in samples from replications. It was seen that LE was superior to N-acetylcystein and cystein in motility, viability, defected acrosome, total morphological abnormalities, HOST and TAC, (P<0.05) except cystein in motility. Neverthless, there was not any statistically difference between LE and control groups (P>0.05). It was conlcluded that there was not any beneficial or detrimental effects of LE on post thawing semen parameters in rams while it was determined that cystein and NAC may have been some detrimental effects on post thawing semen parameters in rams. These results warrants future scientific studies on LE, NAC and cystein in ram semen cryopreservation. Keywords: Ram semen, Cryopreservation, L-Ergothioneine, Cystein, N-acetylcystein, Tushin Koç Spermasının Dondurulması Üzerine L-Ergothionin, N-asetil sistein ve Sisteinin Etkileri ÖzetBu çalışmanın amacı, koçlarda L-Ergothionin (LE), sistein ve N-asetil sisteinin (NAC) farklı dozlarının çözüm sonu sperma parametreleri üzerine etkisini belirlemekti. Dört Tuj ırkı koçtan alınan ejakülatlar değerlendirildi ve 35°C'de karışım yapıldı. Sperma örnekleri, LE (5, 10 mM), Sistein (5, 10 mM), NAC (5, 10 mM) içeren veya antioksidan içermeyen (kontrol), yağsız süt temelli sulandırıcıyla sulandırılarak, 0.25 ml'lik payetlere dolduruldu. Payetler 5°C'ye 2 saat süreyle soğutuldu, sıvı azot buharında (yaklaşık -120°C) 15 dak. süre ile donduruldu ve çözme işlemine kadar sıvı azot içinde saklandı. Payetler su banyosunda (37°C'de 1 dak.) çözüldü. Motilite, canlılık, anormal akrozom, toplam anormalite ve membran bütünlüğü (hipoozmotik şişme testi, HOST) yüzdeleri istatistiksel olarak değerlendirildi. Ayrıca, toplam antioksidan kapasite (TAK) ve toplam oksidatif stres (TOS), replikasyonlardan elde edilen örneklerde değerlendirildi. LE'nin motilite (sistein hariç), canlılık, akrozom hasarı, total morfolojik anormalite, HOST ve TAK oranları bakımından, NAC ve sisteine üstünlük sağladığı görüldü (P<0.05). Yine de, LE ve kontrol grubu arasında istatistiksel bir fark yoktu (P>0.05). Sistein ve NAC'ın koçlarda çözüm sonu spermatolojik parametreler üzerine bazı olumsuz etkileri belirlen...

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Section: Discussionsupporting

confidence: 40%

Farklı Kriyoprotektanların Eritme Sonrası Arı Sperm Kalitesi Üzerine Etkileri

Alçay¹,

Üstüner²,

Çakmak³

et al. 2015

Kafkas Univ Vet Fak Derg

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“…42,44,49,60 Three RCTs evaluated the effects of N-acetyl cysteine alone or in combination with selenium and all three reported a significant improvement in sperm parameters in the treatment arm only. 45,54,61 Several investigators have examined the effect of antioxidant therapy on sperm DNA integrity because sperm DNA damage may be caused, at least in part, by oxidative stress. 15,22,29,53,[62][63][64][65][66][67][68][69] In addition, sperm DNA damage is a more reliable outcome measure than sperm concentration or motility because measures of sperm DNA damage exhibit a lower degree of biological variability than conventional semen parameters.…”

Section: Relationship Between Oxidative Stress and Sperm Dysfunctionmentioning

confidence: 99%

Antioxidant therapy in male infertility: fact or fiction?

Zini¹,

Alhathal²

2011

Asian J Androl

110

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Infertile men have higher levels of semen reactive oxygen species (ROS) than do fertile men. High levels of semen ROS can cause sperm dysfunction, sperm DNA damage and reduced male reproductive potential. This observation has led clinicians to treat infertile men with antioxidant supplements. The purpose of this article is to discuss the rationale for antioxidant therapy in infertile men and to evaluate the data on the efficacy of dietary and in vitro antioxidant preparations on sperm function and DNA damage. To date, most clinical studies suggest that dietary antioxidant supplements are beneficial in terms of improving sperm function and DNA integrity. However, the exact mechanism of action of dietary antioxidants and the optimal dietary supplement have not been established. Moreover, most of the clinical studies are small and few have evaluated pregnancy rates. A beneficial effect of in vitro antioxidant supplements in protecting spermatozoa from exogenous oxidants has been demonstrated in most studies; however, the effect of these antioxidants in protecting sperm from endogenous ROS, gentle sperm processing and cryopreservation has not been established conclusively. Keywords: antioxidant; male infertility; oxidative stress; sperm DNA fragmentation; vitamins RELATIONSHIP BETWEEN OXIDATIVE STRESS AND SPERM DYSFUNCTION Seminal oxidative stress (OS) results from an imbalance between reactive oxygen species (ROS) production and ROS scavenging by seminal antioxidants. Seminal OS is believed to be one of the main factors in the pathogenesis of sperm dysfunction and sperm DNA damage in male infertility.1-4 Indeed, it is estimated that 25% of infertile men possess high levels of semen ROS, whereas fertile men do not have high levels of semen ROS.1,4-6 Although a controlled production of these ROS is required for sperm physiology (sperm hyperactivation, capacitation and acrosome reaction) and for natural fertilization, [7][8][9] the excessive production of ROS by immature germ cells and leukocytes causes sperm dysfunction (lipid peroxidation, loss of motility and sperm DNA damage). 9,10 Spermatozoa are particularly susceptible to oxidative injury due to the abundance of plasma membrane polyunsaturated fatty acids. [10][11][12] These unsaturated fatty acids provide fluidity that is necessary for membrane fusion events (e.g., the acrosome reaction and sperm-egg interaction) and for sperm motility. However, the unsaturated nature of these molecules predisposes them to free radical attack and ongoing lipid peroxidation throughout the sperm plasma membrane. Once this process has been initiated, accumulation of lipid peroxides occurs on the sperm surface (this results in loss of sperm motility) and oxidative damage to DNA can ensue. 13,14SEMINAL ANTIOXIDANT CAPACITY AND SPERM DYSFUNCTION Seminal plasma and spermatozoa themselves are well endowed with an array of protective antioxidants to protect spermatozoa from OS, especially, at the post-testicular level. 6,15,16 Seminal plasma contains a number of high-molecular weigh...

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“…The three most relevant in vivo studies gave conflicting results: Comhaire et al 41 found no improvements in sperm parameters after 3 months' administration of NAC 600 mg day 21 ; whereas the same dosage seemed to improve sperm motility, volume, viscosity and oxidative status in Ciftci et al trial 59 in 2009 with 120 infertile men. Finally, in a trial of 468 patients with idiopathic OAT treated for 6 months with 600 mg day 21 of NAC or 200 mg day 21 of selenium or both, an improvement in sperm count, motility and morphology was seen, with additive beneficial effects when both therapies were administered.…”

Section: Antioxidant Studies Effectmentioning

confidence: 99%

The role of antioxidant therapy in the treatment of male infertility: an overview

Lombardo¹,

Sansone²,

Romanelli³

et al. 2011

Asian J Androl

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In recent years, many studies have focused on the effect of oxidative stress, reactive oxygen species (ROS) and antioxidants on the male eproductive system. Under physiological conditions, sperm produces small amounts of ROS, which are needed for fertilisation, acrosome reaction and capacitation. However, if an increased production of ROS is not associated with a similar increase in scavenging systems, peroxidative damage of the sperm plasma membrane and loss of DNA integrity typically occur, which leads to cell death and reduced fertility. Furthermore, since there is no linear correlation between sperm quality and pregnancy rates, an improvement in semen parameters should not be the sole outcome considered in studies of antioxidant therapies. A definitive conclusion regarding the benefit of these therapies is difficult to obtain, as most of the previous studies lacked control groups, considered different antioxidants in different combinations and doses, or did not evaluate pregnancy rates in previously infertile couples. Even if beneficial effects were reported in a few cases of male infertility, more multicentre, double-blind studies performed with the same criteria are necessary for an increased understanding of the effects of various antioxidants on fertility.

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Effects of N-acetylcysteine on Semen Parameters and Oxidative/Antioxidant Status

Cited by 140 publications

References 21 publications

Farklı Kriyoprotektanların Eritme Sonrası Arı Sperm Kalitesi Üzerine Etkileri

Farklı Kriyoprotektanların Eritme Sonrası Arı Sperm Kalitesi Üzerine Etkileri

Antioxidant therapy in male infertility: fact or fiction?

The role of antioxidant therapy in the treatment of male infertility: an overview

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