Effects of N-acetylcysteine and ethyl pyruvate on ischemia-reperfusion injury in experimental electrical burn model

Turkmen, Süha; Asli, Mutlu; Şahin, Ali; Karaca, Yunus; Menteşe, Ahmet; Demir, Selim; Yuluğ, Esin; Tatli, Özgur; Ari, Neziha Senem; Türedi, Süleyman

doi:10.1016/j.ajem.2016.03.032

Cited by 17 publications

(10 citation statements)

References 22 publications

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“…There are several studies showing antioxidant and antiinflammatory effects of EP. Suha et al (37) investigated the protective effects of NAC and EP in an experimental electric burn model and found that EP had protective effects on heart and brain (37). But in our study, we observed EP alone did not have a therapeutic effect on CO intoxication.…”

Section: Discussioncontrasting

confidence: 71%

The Ameliorative Effects of Ethyl Pyruvate and Dimethyl Sulfoxide on Ischemic Tissue Injury in Experimental Carbon Monoxide Intoxication

Paslı¹,

İmamoğlu²,

Çiçek³

et al. 2020

Eurasian J Emerg Med

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Carbon monoxide (CO) is a colorless, odorless, tasteless, nonirritating gas which normally presents at a level of 0.001% in the atmosphere. It emerges when carbon-based compounds used as fuels for cooking and heating are incompletely consumed, or during fires (1). CO is one of the most important global agents in injury-and intoxication-related mortality (2). Children and the elderly are more rapidly affected. Other factors increasing disposition are exercise, stress, and anemia. High atmospheric concentrations and long-term exposure are other important factors. Due to their high oxygen requirements, brain, heart, and liver are the most susceptible organs to the hypoxic effects of CO exposure (3). Studies have determined that the cardiac effect is associated with Abstract Aim: The purpose of this study was to investigate the protective effects of ethyl pyruvate (EP) and dimethyl sulfoxide (DMSO) on ischemic tissue in brain and cardiac and hepatic tissues in experimentally induced carbon monoxide (CO) intoxication.Material and Methods: Thirty-five mature female Sprague Dawley rats were randomized into five groups of seven animals each. Group 1 received no CO or treatment. Rats in groups 2, 3, 4 and 5 were made to inhale a high-concentration 5000 ppm CO gas mixture for 60 min at 4 L/min. CO levels were then measured from 1 mL tail vein blood. Group 2 received no therapeutic agent, group 3 received 6 mg/kg intraperitoneal (ip) DMSO, group 4 received 50 mg/kg ip EP, group 5 received 50 mg/kg EP and 6 mg/kg DMSO ip. All rats were sacrificed by decapitation. Brain, cardiac and hepatic tissues were removed and histopathological scores were compared. Results:Comparison of group 2 with group 3 and group 2 with group 5 revealed that DMSO alone and EP + DMSO exhibited a reducing effect on degree of cerebral neuronal alteration, degenerative neuron rates, and total cardiac injury score (p=0.005, p=0.002, p=0.004 and p=0.002, p=0.001, p=0.004 respectively). There was no histopathological difference between group 2 and group 4 suggesting EP alone had no therapeutic effect on histopathological injury. Conclusion:Based on our study findings, EP administered alone exhibited no protective effects on the organ injuries investigated, while DMSO exhibited reducing effects on degree of neuron alteration, rates of degenerative neurons, and total cardiac injury scores. An ameliorating effect on cardiac and hepatic injury was more prominent with combined treatment.

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Section: Discussioncontrasting

confidence: 71%

The Ameliorative Effects of Ethyl Pyruvate and Dimethyl Sulfoxide on Ischemic Tissue Injury in Experimental Carbon Monoxide Intoxication

Paslı¹,

İmamoğlu²,

Çiçek³

et al. 2020

Eurasian J Emerg Med

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“…In various animal models of critical illness treatment with EP has been shown to improve survival and/or amelioration of organ dysfunction, e.g. I/R injury in stroke [ 16 , 36 ], I/R injury in an electrical burn model [ 37 ], brain injury [ 38 ], myocardial I/R injury [ 39 ], and whole body radiation-induced injury [ 40 ]. Anti-inflammatory and anti-oxidative action of EP has also been observed in models using human cells, e.g., in cultured Caco-2 transformed human intestinal epithelial cells [ 41 ], in HUVECs [ 42 ], in A549 human transformed pulmonary epithelial cells [ 43 ], and in A549 human alveolar epithelial cells [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Ethyl pyruvate inhibits oxidation of LDL in vitro and attenuates oxLDL toxicity in EA.hy926 cells

et al. 2018

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BackgroundEthyl pyruvate (EP) exerts anti-inflammatory and anti-oxidative properties. The aim of our study was to investigate whether EP is capable of inhibiting the oxidation of LDL, a crucial step in atherogenesis. Additionally, we examined whether EP attenuates the cytotoxic effects of highly oxidized LDL in the human vascular endothelial cell line EA.hy926.MethodsNative LDL (nLDL) was oxidized using Cu2+ ions in the presence of increasing amounts of EP. The degree of LDL oxidation was quantified by measuring lipid hydroperoxide (LPO) and malondialdehyde (MDA) concentrations, relative electrophoretic mobilities (REMs), and oxidation-specific immune epitopes. The cytotoxicity of these oxLDLs on EA.hy926 cells was assessed by measuring cell viability and superoxide levels. Furthermore, the cytotoxicity of highly oxidized LDL on EA.hy926 cells under increasing concentrations of EP in the media was assessed including measurements of high energy phosphates (ATP).ResultsOxidation of nLDL using Cu2+ ions was remarkably inhibited by EP in a concentration-dependent manner, reflected by decreased levels of LPO, MDA, REM, oxidation-specific epitopes, and diminished cytotoxicity of the obtained oxLDLs in EA.hy926 cells. Furthermore, the cytotoxicity of highly oxidized LDL on EA.hy926 cells was remarkably attenuated by EP added to the media in a concentration-dependent manner reflected by a decrease in superoxide and an increase in viability and ATP levels.ConclusionsEP has the potential for an anti-atherosclerotic drug by attenuating both, the oxidation of LDL and the cytotoxic effect of (already formed) oxLDL in EA.hy926 cells. Chronic administration of EP might be beneficial to impede the development of atherosclerotic lesions.

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“…15 In recent years, more and more studies have suggested that NAC has the role of promoting neovascularization, and can reduce the levels of reactive oxygen species (ROS) at the point of injury area. 16,17 Moreover, in theory, the carboxyl group of NAC can form an amide bond with the amino group of CS under the activation of the EDC/NHS system to achieve the purpose of drug loading. 18 Based on the above four materials, we used the electrospinning technology to construct a double-layered patch for the repair of abdominal wall defects.…”

Section: Introductionmentioning

confidence: 99%

Graphene Oxide Functionalized Double-Layered Patch with Anti-Adhesion Ability for Abdominal Wall Defects

Liu

Hou

Liu

et al. 2021

IJN

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Background Effective repair of full-thickness abdominal wall defects requires a patch with sufficient mechanical strength and anti-adhesion characteristics to avoid the formation of hernias and intra-abdominal complications such as intestinal obstruction and fistula. However, patches made from polymers or bio-derived materials may not meet these requirements and lack the bionic characteristics of the abdominal wall. Materials and Methods In this study, we report a consecutive electrospun method for preparing a double-layer structured nanofiber membrane (GO-PCL/CS-PCL) using polycaprolactone (PCL), graphene oxide (GO) and chitosan (CS). To expand the bio-functions (angiogenesis/reducing reactive oxygen species) of the patch (GO-PCL/NAC-CS-PCL), N-acetylcysteine (NAC) was loaded for the repair of full-thickness abdominal wall defects (2×1.5cm) in rat model. Results The double-layered patch (GO-PCL/NAC-CS-PCL) showed excellent mechanical strength and biocompatibility. After 2 months, rats treated with the patch exhibited the desired repair effect with no hernia formation, less adhesion (adhesion score: 1.50±0.50, P<0.001) and more collagen deposition (percentage of collagen deposition: 34.94%±3.31%, P<0.001). Conclusion The double-layered nanomembranes presented in this study have good anti-hernia and anti-adhesion effects, as well as improve the microenvironment in vivo. It, therefore, holds good prospects for the repair of abdominal wall defects and provides a promising key as a postoperative anti-adhesion agent.

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Effects of N-acetylcysteine and ethyl pyruvate on ischemia-reperfusion injury in experimental electrical burn model

Cited by 17 publications

References 22 publications

The Ameliorative Effects of Ethyl Pyruvate and Dimethyl Sulfoxide on Ischemic Tissue Injury in Experimental Carbon Monoxide Intoxication

The Ameliorative Effects of Ethyl Pyruvate and Dimethyl Sulfoxide on Ischemic Tissue Injury in Experimental Carbon Monoxide Intoxication

Ethyl pyruvate inhibits oxidation of LDL in vitro and attenuates oxLDL toxicity in EA.hy926 cells

Graphene Oxide Functionalized Double-Layered Patch with Anti-Adhesion Ability for Abdominal Wall Defects

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